Simultaneous Determination of Hydrochlorothiazide and Triamterene in Capsule Formulations by High-Performance Liquid Chromatography

Menon, Gopi; White, Landy B.

doi:10.1002/jps.2600700931

Cited by 27 publications

(2 citation statements)

References 11 publications

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“…The literature review shows that Triametrene and Hydrochlorothiazide were determined in its bulk powder by derivative ratio spectrophotometry [3], liquid chromatography (LC) [1,4] and chemometric analysis [2].…”

Section: Introductionmentioning

confidence: 99%

Instantaneous determination of antihypertensive drugs utilizing TLC: Densitometric methods

Khorshid¹,

Amin²

2022

Int. J. Front. Life Sci. Res.

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An accurate, sensitive and time saving thin layer chromatography (TLC) - densitometric method has been developed and validated for determination of two Antihypertensive drug mixtures. Mixture one contains Spironolactone, Furosemide and Anthranilic acid while, mixture two contains Triamterene, Hydrochlorothiazide and chlorothiazide. Mixture 1 uses developing system consist of Hexane, Ethyl acetate and Glacial acetic acid (5ml – 5ml – 0.1ml ) as developing system and 240 nm as scanning wavelength for separation and determination of Spironolactone, Furosemide and Anthranilic acid (metabolite of Furosemide) and mixture 2 uses developing system consist of Ethyl acetate, Methanol, Glacial acetic acid and Ammonium hydroxide (8ml - 1ml - 0.2ml - 0.3 ml) as developing system and 300nm as scanning wave length for separation and determination of Triamterene, Hydrochlorothiazide and Chlorothiazide ( impurity of Hydrochlorothiazide). This method was validated and shown to demonstrate good accuracy and precision according to the International Council for Harmonisation (ICH) guidelines.

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Section: Introductionmentioning

confidence: 99%

Instantaneous determination of antihypertensive drugs utilizing TLC: Densitometric methods

Khorshid¹,

Amin²

2022

Int. J. Front. Life Sci. Res.

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“…Because NSAIDs are the commonly used drugs, monitoring these compounds in pharmaceutical samples is very important. Several methods are described in literature for NSAIDs in the raw material and dosage forms, including potentiometric titration [1], high-performance liquid chromatography [2][3][4][5][6][7][8][9][10][11][12], GC/MS analysis [13,14], and spectrofluorometric determination [15,16]. In the United State Pharmacopoeia [17] the liquid chromatography methods with a 4.6-mm × 25-cm column that contains packing Octadecyl silane chemically bonded to porous silica or ceramic microparticles, 3 to 10μm are described for the routine determination of ibuprofen and mefenamic acid in pure form and capsules.…”

Section: Introductionmentioning

confidence: 99%

HPLC separation of ibuprofen and mefenamic acid using molecularly imprinted polymer as stationary phase

Hung

Huang

et al. 2006

E-Polymers

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Abstract:A simple high-performance liquid chromatographic method for the assay of ibuprofen and mefenamic acid in tablets is described. This method was developed by using self-prepared molecularly imprinted polymers (MIPs) as the stationary phase. MIPs are synthesized using non-covalent method by the free radical polymerization of template, functional monomer and an excess of crosslink monomers resulting in organic materials. The resulting bulk material was then ground into 25~44μm particles and packed into analytical columns. The effect of functional monomers/crosslinking ratio and the concentration of acetonitrile in the mobile phase were studied. Ibuprofen and mefenamic acid were efficiently resolved on the polymer. The retention time of ibuprofen and mefenamic acid were approximately 19.69±0.36min and 46.27±1.23min, respectively. Linearity was observed from 3.13-8.33g/l for ibuprofen and 0.3-0.9g/l for mefenamic acid, with the coefficients of determination (r 2 ) of 0.9977 and 0.9958. The limits of detection (LOD) of ibuprofen and mefenamic acid were found to be respectively 0.42g/l and 0.06g/l, while the limits of quantitation (LOQ) were 1.41g/l and 0.20g/l, respectively. The self-prepared MIP was successfully applied to the commercial tablet analysis and the result showed a good accuracy with -1.33 ~ -1.8% and -0.8 ~ +1.33% for ibuprofen and mefenamic acid.

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Validated spectroscopic methods for the estimation of triamterene in bulk powder and capsule form via derivatization reactions

et al. 2018

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Sensitive, simple and specific spectrophotometric and spectrofluorimetric methods were developed for the determination of triamterene in bulk powder and pharmaceutical dosage form. The spectrophotometric method (Method A) is based on the formation of an ion‐pair complex with eosin Y in acetate buffered solution of pH 3.7 followed by measuring the absorbance at 545 nm. The absorbance–concentration plot is rectilinear over the range 3.0–15.0 μg/mL with a limit of detection (LOD) of 0.429 μg/mL and a limit of quantitation (LOQ) of 1.031 μg/mL. The spectrofluorimetric method (Method B) is based on the reaction of triamterene with 7‐ chloro‐4‐nitrobenzo‐2‐oxa‐1,3‐diazole (NBD‐Cl) in basic solution (pH 10) to form a product with high fluorescence measured at 546 nm after being excited at 438 nm. The plot of fluorescence versus concentration is linear within the range 2.0–10.0 μg/mL. The suggested methods were applied for the analysis of commercial capsules containing the studied drug with successful results. The results obtained from the proposed method were statistically compared with those of a reported one and revealed good agreement. The presented methods are useful in routine analysis of triamterene in laboratories of quality control.

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Simultaneous Determination of Hydrochlorothiazide and Triamterene in Capsule Formulations by High-Performance Liquid Chromatography

Cited by 27 publications

References 11 publications

Instantaneous determination of antihypertensive drugs utilizing TLC: Densitometric methods

Instantaneous determination of antihypertensive drugs utilizing TLC: Densitometric methods

HPLC separation of ibuprofen and mefenamic acid using molecularly imprinted polymer as stationary phase

Validated spectroscopic methods for the estimation of triamterene in bulk powder and capsule form via derivatization reactions

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