Instantaneous determination of antihypertensive drugs utilizing TLC: Densitometric methods

Abstract

An accurate, sensitive and time saving thin layer chromatography (TLC) - densitometric method has been developed and validated for determination of two Antihypertensive drug mixtures. Mixture one contains Spironolactone, Furosemide and Anthranilic acid while, mixture two contains Triamterene, Hydrochlorothiazide and chlorothiazide. Mixture 1 uses developing system consist of Hexane, Ethyl acetate and Glacial acetic acid (5ml – 5ml – 0.1ml ) as developing system and 240 nm as scanning wavelength for separation and determination of Spironolactone, Furosemide and Anthranilic acid (metabolite of Furosemide) and mixture 2 uses developing system consist of Ethyl acetate, Methanol, Glacial acetic acid and Ammonium hydroxide (8ml - 1ml - 0.2ml - 0.3 ml) as developing system and 300nm as scanning wave length for separation and determination of Triamterene, Hydrochlorothiazide and Chlorothiazide ( impurity of Hydrochlorothiazide). This method was validated and shown to demonstrate good accuracy and precision according to the International Council for Harmonisation (ICH) guidelines.