HPLC separation of ibuprofen and mefenamic acid using molecularly imprinted polymer as stationary phase

Abstract

Abstract:A simple high-performance liquid chromatographic method for the assay of ibuprofen and mefenamic acid in tablets is described. This method was developed by using self-prepared molecularly imprinted polymers (MIPs) as the stationary phase. MIPs are synthesized using non-covalent method by the free radical polymerization of template, functional monomer and an excess of crosslink monomers resulting in organic materials. The resulting bulk material was then ground into 25~44μm particles and packed into analytical columns. The effect of functional monomers/crosslinking ratio and the concentration of acetonitrile in the mobile phase were studied. Ibuprofen and mefenamic acid were efficiently resolved on the polymer. The retention time of ibuprofen and mefenamic acid were approximately 19.69±0.36min and 46.27±1.23min, respectively. Linearity was observed from 3.13-8.33g/l for ibuprofen and 0.3-0.9g/l for mefenamic acid, with the coefficients of determination (r 2 ) of 0.9977 and 0.9958. The limits of detection (LOD) of ibuprofen and mefenamic acid were found to be respectively 0.42g/l and 0.06g/l, while the limits of quantitation (LOQ) were 1.41g/l and 0.20g/l, respectively. The self-prepared MIP was successfully applied to the commercial tablet analysis and the result showed a good accuracy with -1.33 ~ -1.8% and -0.8 ~ +1.33% for ibuprofen and mefenamic acid.

Molecular imprinting science and technology: a survey of the literature for the years 2004-2011

“…Efforts to employ MIPs in assays have extended well beyond the large volume of literature focused upon MIPSE, most notably CE and CEC, and LC . In contrast to the 1990s and early 2000s, the proportion of studies devoted to, or heavily dependent upon, LC has reduced significantly.…”

Synthesis and Adsorption Performance of Molecularly Imprinted Microspheres for Quercetin by Suspension Polymerization

“…Molecularly imprinted polymers (MIPs) possess high selectivity toward the target molecules (or the template molecules) due to the "lock-key" relationship between MIPs and the target molecules [8][9][10]. MIPs have been widely applied in drug separation, food and environmental testing, antibody or receptor analogues, sensors and many other fields, which have shown good prospects [11][12][13][14][15][16][17][18][19].…”