Simultaneous determination of dopamine and 3,4-dihydroxyphenylacetic acid in mouse striatum using mixed-mode reversed-phase and cation-exchange high-performance liquid chromatography

Tsunoda, Makoto; Aoyama, Chiaki; Nomura, Hiroshi; Toyoda, Takeshi; Matsuki, Norio; Funatsu, Takashi

doi:10.1016/j.jpba.2009.09.045

Cited by 51 publications

(30 citation statements)

References 19 publications

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“…Therefore there is a need for their quantitative determination in body fluids in order to identify any endocrine disorders and other physiological and pathological abnormality in the body at an early stage [2]. The most common method for analysis of catecholamines in biological fluids is RPLC in conjunction with various detection systems like mass spectrometry (MS), electrochemical detection (ECD) and fluorescence detection [2,[5][6][7][8][9].…”

Section: Introductionmentioning

confidence: 99%

Determination of catecholamines in urine using hydrophilic interaction chromatography with electrochemical detection

Kumar

Hart

McCalley

2011

Journal of Chromatography A

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Section: Introductionmentioning

confidence: 99%

Determination of catecholamines in urine using hydrophilic interaction chromatography with electrochemical detection

Kumar

Hart

McCalley

2011

Journal of Chromatography A

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“…A challenging column separation of catecholamines and their metabolites was performed employing fluorescence derivatization using a cation-exchange/reversed-phase (mixedmode) column. 11 The reported mixed-mode column using pH gradient elution achieved adequate LC separation, while the imidazole-derivatization of catecholamines involved tedious analytical procedures. 11 Thus, to establish a convenient and direct assay for catecholamines and their metabolites, an LC-MS-based assay, which used a cation-exchange/reversed-phase mixed-mode column, was proposed in this study.…”

Section: Introductionmentioning

confidence: 99%

Analysis of Catecholamine and Their Metabolites in Mice Brain by Liquid Chromatography-Mass Spectrometry Using Sulfonated Mixed-mode Copolymer Column

et al. 2018

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In this study, a simultaneous assay for catecholamines and their metabolites in the brain was established using liquid chromatography-mass spectrometry (LC-MS). To achieve complete separation, a cation-exchange/reversed-phase mixedmode copolymer resin column containing 0.81 wt% sulfo groups was used for the simultaneous LC-MS assay. The analyzed catecholamines were dopamine (DA), norepinephrine (NE), and epinephrine (E), while the metabolites lacking amino groups were 3,4-dihydroxyphenylacetic acid (DOPAC), homovanillic acid (HVA), and 3-methoxy-4hydroxyphenylglycol (MHPG). The metabolites were separated and detected using LC-MS, on columns with and without sulfo groups. However, we could not achieve adequate separation of catecholamines on both columns using a gradient elution of 0 -50 (v/v)% methanol containing 0.1 (v/v)% formic acid (FA). When volatile ion-pairing reagents were added to the mobile phase, they improved the retention and detection of catecholamines on the sulfonated mixed-mode column. Under optimized elution conditions, which involved a linear gradient elution of water containing 0.1 (v/v)% FA to 50 (v/v)% acetonitrile in 50 mM ammonium formate at 40 C and a 0.20 mL/min rate, all six target molecules were simultaneously detected within 25 min, when using negative mode LC-MS on a sulfonated mixed-mode column. The limits of detection (LODs) for DA, NE, E, DOPCA, HVA, and MHPG were determined to be 20. 7, 12.6, 74.6, 1110, 18.7, and 3196 nM, respectively. Moreover, the established LC-MS assay allowed the detection of endogenous DA, NE, and HVA, in normal mouse brain samples at concentrations higher than 20, 9, and 4 pmol/mg, respectively.

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“…Different pervious works have been reported for determination of DA. To mention some of them: chromatography [8,9], spectrophotometry [10][11][12], fluorescence [13], chemiluminescence [14,15], and electroanalysis [1][2][3][4][5][6][16][17][18][19]. Electrochemical techniques have attracted great concern over other analytical methods as the analysis is fast, low in cost, high accuracy and low detection limit [1][2][3][4][5][6][7]20].…”

Section: Introductionmentioning

confidence: 99%

Selective electrochemical determination of dopamine at p-nitroaniline film-hole modified glassy carbon electrodes

Olana¹,

Kitte²,

Geletu³

et al. 2018

Bull. Chem. Soc. Eth.

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ABSTRACT. Herein, we report determination of dopamine (DA) at modified glassy carbon electrode (GCE) with a film produced by reduction of diazonium generated from p-nitroaniline (PNA). Pores were created purposely by stripping pre-deposited gold nanoparticles (AuNPs) in the modifier film. The modified electrodes were characterized electrochemically by common redox probes: hydroquinone (HQ), hexacyanoferrate [(Fe(CN) . Comparison was made for the cyclic voltammetric and amperometric response of DA using the modified electrodes against the bare GCE in phosphate buffer solution (PBS) of pH 7.5. The bare and modified GCE showed a linear response to DA in the concentration range of 0.2-2.2 mM and 5-35 µM with detection limit of 0.015 mM and 0.6112 µM, respectively. The modified electrode showed high sensitivity, well selectivity, good anti-interference ability, durable stability and good electrode reproducibility for determining DA. The reported modified electrode is a promising sensor for use in electroanalysis of DA.

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Simultaneous determination of dopamine and 3,4-dihydroxyphenylacetic acid in mouse striatum using mixed-mode reversed-phase and cation-exchange high-performance liquid chromatography

Cited by 51 publications

References 19 publications

Determination of catecholamines in urine using hydrophilic interaction chromatography with electrochemical detection

Determination of catecholamines in urine using hydrophilic interaction chromatography with electrochemical detection

Analysis of Catecholamine and Their Metabolites in Mice Brain by Liquid Chromatography-Mass Spectrometry Using Sulfonated Mixed-mode Copolymer Column

Selective electrochemical determination of dopamine at p-nitroaniline film-hole modified glassy carbon electrodes

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