Determination of catecholamines in urine using hydrophilic interaction chromatography with electrochemical detection

Kumar, Abhinav; Hart, John P.; McCalley, David V.

doi:10.1016/j.chroma.2011.04.034

Cited by 72 publications

(53 citation statements)

References 26 publications

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“…Generally, asymptomatic levels of the catecholamines are 0 nM to 10 nM (0-1750 pg/mL) in urine and 0.19 nM to 11 nM (33.25-1925 pg/mL) in blood (Kumar et al, 2011;Mayo Clinic Laboratories, 2012). As a result of trauma, catecholamine blood concentrations fluctuate and can reach levels of 29 nM (49 ng/mL) or higher, depending on severity (Hamill et al, 1987).…”

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confidence: 99%

“…However, the current method to measure catecholamine levels is conducted in clinical laboratories by high-performance liquid chromatography (HPLC) on blood or urine samples (Mayo Clinic Laboratories, 2012;Tsunoda et al, 2009). Other developed non-clinical methods of catecholamine detection include electrochemical techniques in conjunction with various nanosensors or coatings, and purification methods such as hydrophilic interaction chromatography (Atta et al, 2010;Kim et al, 2010;Kumar et al, 2011;Prasad et al, 2009). Disadvantages of these methods include the need for a clinical chemistry laboratory to conduct the assay, time and resource costs for sample transport, stabilizing reagents and purification steps (Atta et al, 2010;Kumar et al, 2011;Mayo Clinic Laboratories, 2012;Prasad et al, 2009).…”

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confidence: 99%

“…Other developed non-clinical methods of catecholamine detection include electrochemical techniques in conjunction with various nanosensors or coatings, and purification methods such as hydrophilic interaction chromatography (Atta et al, 2010;Kim et al, 2010;Kumar et al, 2011;Prasad et al, 2009). Disadvantages of these methods include the need for a clinical chemistry laboratory to conduct the assay, time and resource costs for sample transport, stabilizing reagents and purification steps (Atta et al, 2010;Kumar et al, 2011;Mayo Clinic Laboratories, 2012;Prasad et al, 2009). To be useful in situations where the symptoms are transient, catecholamine levels would need to be measured frequently and rapidly, even at the bedside, which current assay methods do not permit.…”

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confidence: 99%

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Development of electrochemical methods to enzymatically detect traumatic brain injury biomarkers

Haselwood

Belle

2015

Biosensors and Bioelectronics

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As awareness of traumatic brain injury (TBI) increases, the need to detect mild forms and distinguish between the different severities of TBI becomes more apparent. The goal of this work is to develop a point-of-care sensor to detect whole blood biomarkers for rapid and sensitive diagnosis of TBI severity. Presented herein is the enzymatic detection of norepinephrine through the use of immobilization chemistry and impedance techniques. Sustained elevation of norepinephrine concentrations in the blood has been correlated to negative long-term outcomes in TBI cases, often resulting in permanent cognitive or physical deficits.Novel analysis techniques have been used to identify an optimal binding frequency (371.1 Hz) of norepinephrine to the immobilized enzyme on a gold disk electrode. This form of analysis yielded a logarithmic fit characterized by exceptional responsivity (20.89 ohms/pgmL -1 ), reproducibility (R 2 = 0.96), and lower limit of detection (98 pg/mL) first in purified samples, then in rabbit whole blood solutions. Once the optimal binding frequency was determined, the preliminary use of an impedance-time technique was attempted in this work. This technique more closely resembles the amperometric detection method used in commercial self-monitoring blood glucose meters, allowing for continuous or instantaneous measurement of blood borne biomarkers without compromising sensitivity. Future directions include exploration of simultaneous multi-marker detection with the impedance-time technique and experimentation with novel mesoporous materials to filter large blood components.

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Development of electrochemical methods to enzymatically detect traumatic brain injury biomarkers

Haselwood

Belle

2015

Biosensors and Bioelectronics

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“…The majority of methods have been based on reverse phase chromatography. However, it is believed that more work will be reported on the application of techniques such as hydrophilic interaction chromatography (HILIC) [87]. Similarly, the combination of electrochemistry with mass spectrometry and LC/MS [88] will be become an increasingly important area for drug metabolism studies.…”

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confidence: 99%

Review: The Application of Liquid Chromatography Electrochemical Detection for the Determination of Drugs of Abuse

Honeychurch

2016

Separations

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This review (4 tables, 88 references) describes current developments in the design and application of liquid chromatography electrochemical detection (LC ED) based approaches for the determination of drugs of abuse. Specific emphasis is placed on operating details and performance characteristics for selected applications. LC ED has been shown to be highly sensitive and specific as well being a more economic option. A wide range of abused substances have been determined using this approach, including: cannabinoids, ethanol, opiates, morphine, mushroom toxins, benzodiazepines and several legal highs. Reverse-phase liquid chromatography with either amperometric or coulometric determination has been the most commonly reported applications. However, coulometric arrays have been also reported. Detection limits in the ng/mL region have been reported for most target analytes.

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“…However, recent studies have shown the possibility of utilising related techniques such as hydrophilic interaction liquid chromatography with electrochemical detection [53]. Table 1 gives a summary of the liquid chromatographic approaches that have been reported prior to 2006.…”

Section: Liquid Chromatography Electrochemical Detection Of Benzodiazmentioning

confidence: 99%

Electrochemical Detection of Benzodiazepines, Following Liquid Chromatography, for Applications in Pharmaceutical, Biomedical and Forensic Investigations

Honeychurch¹,

Hart²

2014

insc.

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Abstract:Benzodiazepines are an important class of drugs commonly administered with a potential for abuse and environmental pollution. This review focuses on the liquid chromatographic electrochemical detection of the benzodiazepine class of drugs. These are characterised by a readily electrochemically reducible azomethine group, with a number also substituted by other electrochemically active groups. Liquid chromatography employing both single and dual electrode detection has been reported for a variety of benzodiazepines and their metabolites in biological, pharmaceutical, biomedical and forensic investigations. Recently, electrochemistry has been utilised to mimic biological oxidation processes and has been combined with liquid chromatography/mass spectroscopy for their identification and quantification of the products generated. The present review focuses on recent developments in liquid chromatographic-electrochemical determination of benzodiazepines reported since 2006, with earlier reports given in summary.

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Determination of catecholamines in urine using hydrophilic interaction chromatography with electrochemical detection

Cited by 72 publications

References 26 publications

Development of electrochemical methods to enzymatically detect traumatic brain injury biomarkers

Development of electrochemical methods to enzymatically detect traumatic brain injury biomarkers

Review: The Application of Liquid Chromatography Electrochemical Detection for the Determination of Drugs of Abuse

Electrochemical Detection of Benzodiazepines, Following Liquid Chromatography, for Applications in Pharmaceutical, Biomedical and Forensic Investigations

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