Simultaneous Determination of Acetylsalicylic Acid and Caffeine in Pharmaceutical Formulation by First Derivative Synchronous Fluorimetric Method

Abstract

A sensitive, rapid, and specific assay has been developed for the simultaneous determination of acetylsalicylic acid and caffeine in commercial tablets based on their natural fluorescence. The mixture of these drugs was resolved by first derivative synchronous fluorimetric technique using two scans. At Deltalambda=106 nm, using first derivative synchronous scanning, only acetylsalicylic acid yields a detectable signal at 316 nm (peak to zero method) which is unaffected by caffeine. At Deltalambda=30 nm, the signal of caffeine at 288 nm (peak to zero method) is not affected by acetylsalicylic acid. The range of application is between 0.021 and 41.62 microg ml(-1) (correlation coefficient, R=0.9995) for acetylsalicylic acid and between 0.4486 and 44.86 microg ml(-1) (correlation coefficient, R=0.99786) for caffeine. The recovery range of 98.40-102% for acetylsalicylic acid and 90-100.5% for caffeine from their synthetic mixture was reported. Overall recovery of both compounds about 97-99% for acetylsalicylic acid and 97-98% for caffeine was obtained from real sample analysis. The detection limits are 0.0013 microg ml(-1) and 0.0306 microg ml(-1) for acetylsalicylic acid and caffeine, respectively. The relative standard deviation (n=10) for 20 microg ml(-1) of acetylsalicylic acid is 2.75% and for 2.2 microg ml(-1)of caffeine is 1.7%.

A Synchronous Fluorescence Spectrofluorometric Method for the Simultaneous Determination of Clonazepam and Paroxetine Hydrochloride in Combined Pharmaceutical Dose Form

“…The combination of synchronous scanning spectrofluorimetry with derivative techniques is advantageous in terms of sensitivity, spectral discrimination, and more reliable identification of chemical species in multi component analysis. 14,15 The goal of the present work was to develop a simple, cost effective, sensitive, and rapid method for the simultaneous determination of CLO and PH in tablet form through firstderivative synchronous fluorimetry (FDSF) based on their native fluorescence. The emission spectra of CLO and PH overlap, which makes it difficult to analyze and determine their contents by conventional fluorimetry.…”

Novel ultrasensitive and ultrafast voltammetric determination of biological aminochromes on the copper nanodoped mercury monolayer carbon fiber electrode

“…In other words, the reduction process can involve participation of 1-3 or 4 electrons and protons (that is equivalent to 1-3 or 4 hydrogen atoms). To clarify this fact we have compared the influence on the Cu 2+/0 -sytem of similar compounds (o-BQ, able to accept two hydrogen atoms) or a product of soft dopamine oxidation (using Hg 2+ ions [23]) having a yellow colour which differs from the red coloured DAC and is therefore able to accept less than four electrons (hydrogen atoms). The voltammograms of these compounds differ substantially (Fig.…”

Determination of three analgesics in pharmaceutical and urine sample on nano poly (3, 4-ethylenedioxythiophene) modified electrode

“…Ultraviolet, fluorescence, FT-Raman spectroscopy and infrared spectrofluorimetric methods were described for the determination of ASA and salicylic acid in pharmaceutical preparations [21,22]. Simultaneous determination of acetylsalicylic acid and caffeine in pharmaceutical formulation were reported by first derivative synchronous fluorimetric method [23]. The electrochemical oxidation of salicylic acid in pharmaceutical formulations of ASA was studied on a glassy carbon electrode using cyclic voltammetric and differential pulse voltammetric method [24].…”