Simultaneous determination of 3,4-dihydroxyphenylacetic acid and homovanillic acid using high performance liquid chromatography–fluorescence detection and application to rat kidney microdialysate

Simultaneous determination of dopamine and 3,4-dihydroxyphenylacetic acid in mouse striatum using mixed-mode reversed-phase and cation-exchange high-performance liquid chromatography

“…The limits of quantification (a signal-to-noise ratio of 10) were 300 and 1500 fmol for dopamine and DOPAC, respectively. These values were higher than those previously reported [7,10], and were sufficient for the simultaneous determination of dopamine and DOPAC in mouse striatum.…”

“…The determination methods for catecholamines, namely, norepinephrine, epinephrine and dopamine [7][8][9], and for DOPAC and homovanillic acid (HVA) [10] have already been developed. Dopamine and DOPAC can be determined with these methods.…”

HPLC/MS/MS for quantification of two types of neurotransmitters in rat brain and application: Myocardial ischemia and protection of Sheng-Mai-San

“…Currently many bioanalytical techniques have been developed for the analysis of monoaminergic neurotransmitters and neuropeptides. GC [8,9], CE [10,22], LC-UV [11,12] and LCelectrochemical [13,14] fluorescent detection [14][15][16][17] have been reported to determine monoaminergic neurotransmitter and neuropeptide levels. However, most of these methods require derivatization of the analytes, the use of ion-pairing reagents, and large quantities of samples.…”

Highly sensitive and selective derivatization-LC method for biomolecules based on fluorescence interactions and fluorous separations

“…For example, benzylamine for 5-hydroxyindoles [25][26][27], 1,2-diphenylethylenediamine [28,29] and ethylenediamine [30][31][32] for catecholamines, 1,2-diamino-4,5-dimethoxybenzene [33,34] and 1,2-diamino-4,5-methylenedioxybenzene [35,36] for ␣-sialic acid are widely used in biological research and diagnosis.…”