Simultaneous determination of 10 active components in traditional Chinese medicine “YIGONG” capsule by RP-HPLC–DAD

Feng, Chao; Cai, Yangyang; Ruan, Jinlan

doi:10.1016/j.jpba.2008.01.019

Cited by 21 publications

(8 citation statements)

References 18 publications

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“…According to the theory and medicinal practice of TCM, all the medicinal compositions play important roles in the therapeutic effects. Therefore, selecting multiple constituents from different medicinal herbs as evaluation markers has been gradually applied for the control of TCM (Yin et al, 2009;Chen et al, 2009;Feng et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Simultaneous quantification of twelve active components in Yiqing granule by ultra‐performance liquid chromatography: application to quality control study

Chen

Yang

Shi

2010

Biomedical Chromatography

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An ultra-performance liquid chromatography (UPLC) method coupled with photodiode array (PDA) detection has been developed and validated for simultaneous quantification of 12 active components (berberine, palmatine, jatrorrhizine, aloe-emodin, rhein, emodin, chrysophanol, physcion, baicalin, baicalein, wogonoside and wogonin) in Yiqing granule. Optimum separation were achieved on a C(18) column (50 × 2.1 mm i.d., 1.7 µm particle) through a 7.5 min gradient delivery of a mixture of A (acetonitrile) and B (0.1% aqueous phosphoric acid containing 1.8 mmol/L sodium dodecyl sulfonate and 10% acetonitrile, v/v) at a flow rate of 0.3 mL/min at 30°C. Because of the different UV characteristics of these components, three detection wavelengths were used for quantitative analysis. All of the analytes showed good linearity (r of >0.999). The method was validated for repeatability, precision, stability, accuracy and selectivity. The validated method was applied to quality control of Yiqing granule from different production batches.

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Section: Introductionmentioning

confidence: 99%

Simultaneous quantification of twelve active components in Yiqing granule by ultra‐performance liquid chromatography: application to quality control study

Chen

Yang

Shi

2010

Biomedical Chromatography

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“…The intra‐assay precision was performed on a single day with an interval of 4 h, and the inter‐assay precision was performed over 3 days. The injection of six samples, which were obtained through the same sample preparation procedure, was used to test analysis repeatability (Feng et al ., 2008). The same real sample after preparation was analysed after 0, 4, 8, 12, 16 and 24 h at room temperature in order to test stability.…”

Section: Methodsmentioning

confidence: 99%

Simultaneous analysis of three bioactive compounds in Artemisia annua hairy root cultures by reversed‐phase high‐performance liquid chromatography–diode array detector

Zhai

Zhong

2010

Phytochemical Analysis

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“…To measure accuracy, recovery test was carried out (Qian et al, 2007;Feng et al, 2008;Wang et al, 2009;Yin et al, 2009). By measuring each five concentrations of four marker component, a calibration curve was established.…”

Section: Analysis Methods Validationmentioning

confidence: 99%

Simultaneous determination of four marker components in Yukmijihwang Tang by high performance liquid chromatography/diode array detector

Won

Yeul

2010

Arch. Pharm. Res.

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Simultaneous determination method of four marker components, paeoniflorin, loganin, 5-hydroxymethyl-2-furaldehyde and paeonol in traditional herbal medicine, Yukmijihwang tang, was developed to achieve rapidly and systematic quality control by high performance liquid chromatography coupled with diode array detection. To develop this high performance liquid chromatography method, C(18) column (5 microm, 120 A, 4.6 mm x 150 mm) was used with gradient elution of water and methanol as mobile phase. Validation of the chromatography method was evaluated by linearity, recovery, and precision test. Calibration curves of standard components showed excellent linearity with R(2) > 0.9990. Limits of detection and limits of quantification were in the ranges 0.01-0.02 and 0.04-0.07 microg/mL, respectively. The relative standard deviations of data of the intra-day and inter-day experiments were less than 1.15% and 3.76%, respectively. The results of recovery test were found to range from 94.88 to 107.43% with relative standard deviation values 0.07-2.66%. The results of validation suggested that this method was very accurate and stabilized.

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Simultaneous determination of 10 active components in traditional Chinese medicine “YIGONG” capsule by RP-HPLC–DAD

Cited by 21 publications

References 18 publications

Simultaneous quantification of twelve active components in Yiqing granule by ultra‐performance liquid chromatography: application to quality control study

Simultaneous quantification of twelve active components in Yiqing granule by ultra‐performance liquid chromatography: application to quality control study

Simultaneous analysis of three bioactive compounds in Artemisia annua hairy root cultures by reversed‐phase high‐performance liquid chromatography–diode array detector

Simultaneous determination of four marker components in Yukmijihwang Tang by high performance liquid chromatography/diode array detector

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