Simultaneous analysis of three bioactive compounds in <i>Artemisia annua</i> hairy root cultures by reversed‐phase high‐performance liquid chromatography–diode array detector

Zhai, Dan‐Dan; Zhong, Jian‐Jiang

doi:10.1002/pca.1226

Cited by 17 publications

(7 citation statements)

References 24 publications

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“…Artemisia annua ranks high among medicinal plants having the capacity to offer phyto‐protective activity against numerous diseases of humans and animals. This potential is conferred on it undoubtedly by its rich portfolio of medicinally important bioactive compounds including several sesquiterpenoids, flavonoids, terpene allylic hydroperoxides and endoperoxides (Brown, ; Zhai and Zhong, ). Pine needles of C. deodara are acknowledged as a healthy food with abundant contents of protein, vitamins and minerals (Zhang et al., ).…”

Section: Discussionmentioning

confidence: 99%

Effects of Chinese herbal mixture on performance, egg quality and blood biochemical parameters of laying hens

Wang

et al. 2016

Animal Physiology Nutrition

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The aim of the study was to evaluate the effect of Chinese herbal mixture (CHM) in laying hen diets on performance, egg quality and egg nutrition components, as well as selected blood biochemical parameters. A total of 270 Lohmann Brown laying hens, 24 week of age, were randomly distributed into three dietary treatments and fed for 6 week. The CHM was used at the levels of 0%, 0.5% and 1.0% in the diets of the control and two treatment groups respectively. Each dietary treatment was divided into five replicates, comprising of 18 hens each. Hens were housed in a 3-tier battery system. Feed and water were provided ad libitum. Blood samples and eggs were collected at the end of the experiment. The results showed that dietary treatments did not affect egg weight, mortality, eggshell thickness, albumen height, Haugh units, eggshell-breaking strength and egg-shape index. Dietary supplementation of CHM significantly improved (p < 0.05) laying rate and the lowest feed conversion ratio were observed with 1.0% CHM supplementation diets. CHM supplementation decreased (p < 0.05) cracked-egg rate, egg yolk cholesterol, blood serum levels of cholesterol, triglyceride, low-density lipoprotein cholesterol (LDL-C) and alanine aminotransferase (ALT) and increased blood serum levels of high-density lipoprotein cholesterol (HDL-C). No differences in serum total protein, serum albumen, serum glucose and aspartate amino transferase (AST) were observed in hens fed CHM. In conclusion, dietary supplementation with CHM improved egg production and egg quality and reduced serum cholesterol concentrations in laying hens. Dietary supplementation of 1.0% CHM in layer diets may be a feasible means of producing eggs with lower cholesterol and higher egg yolk phospholipid contents for health conscious consumers.

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Section: Discussionmentioning

confidence: 99%

Effects of Chinese herbal mixture on performance, egg quality and blood biochemical parameters of laying hens

Wang

et al. 2016

Animal Physiology Nutrition

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“…MIC for ponticaepoxide was determined to be in the range 100−200 µg/mL, whereas aliquots of authentic reference artemisinin showed no activity up to 2000 µg/mL. Antibacterial activity has not previously been demonstrated for the polyacetylene ponticaepoxide, although it is known to have insecticidal properties (19). Fraction 5 of the DCM extract (MIC between 300 and 600 µg/mL) contained primarily 2,4-dihydroxy-6-methoxyacetophenone (83%) and a minor constituent, which was tentatively identified as 3,3′,5-trihydroxy-4′,6,7-trimethoxyflavone (eupatin; 20) by LC-DAD/APCI-MS. From this fraction only 2,4-dihydroxy-6methoxyacetophenone was isolated in pure form and identified by NMR spectroscopy and LC-DAD/APCI-MS/MS.…”

Section: Isolation Of Antibacterial Compounds From Active Fractionsmentioning

confidence: 92%

Bioassay-Guided Chromatographic Isolation and Identification of Antibacterial Compounds from Artemisia annua L. That Inhibit Clostridium perfringens Growth

Ivarsen

Fretté

Christensen

et al. 2014

Journal of AOAC INTERNATIONAL

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Clostridium perfringens is the causative agent of necrotic enteritis leading to significant losses in the poultry industry. Dichloromethane and n-hexane extracts of aerial parts of Artemisia annua (Asteraceae) exhibited activity against C. perfringens with minimum inhibitory concentrations (MIC) of 185 and 270 μg/mL, respectively. Bioassay-guided fractionation of the extracts gave several active fractions (MIC between 75 and 600 μg/mL). Investigations of the most active fractions resulted in the isolation and characterization of the polyacetylene ponticaepoxide (MIC between 100 and 200 μg/mL) and (+)-threo-(5E)-trideca-1,5-dien-7,9,11-triyne-3,4-diol (MIC between 400 and 800 μg/mL), the flavonols chrysosplenol D (MIC between 200 and 400 μg/mL) and casticin (slight inhibition at 800 μg/mL), and 2,4-dihydroxy-6-methoxyacetophenone (slight inhibition at 800 μg/mL). Also, the coumarin scopoletin and the sesquiterpene lactone artemisinin were isolated from active fractions but showed no inhibition of C. perfringens growth at 800 and 2000 μg/mL, respectively. Fractions containing essential oil components with camphor constituting >60% did not show inhibition of C. perfringens up to 1600 μg/mL. Extracts and some active fractions showed higher antibacterial effect than individual bioactive compounds, suggesting that synergistic effects may underlie the observed antibacterial effect. The present study adds new valuable information on the antibacterial effect of A. annua against C. perfringens.

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“…The structure of this compound was established by spectroscopic methods and elucidated to be 9-epipectachol B (80) (Kisiel and Stojakowska 1997). Similarily drimartol A (66) was isolated from hairy root cultures of Artemisia annua (Zhai and Zhong 2010). Jatrophadioxan (81) was isolated as a new compound from Jatropha integrrima (Euphorbiaceae) (Sutthivaiyakit et al 2009 …”

Section: Scopoletin-derived Sesquiterpene Ethersmentioning

confidence: 99%

Sesquiterpene coumarins

Gliszczyńska

Brodelius

2011

Phytochem Rev

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Plants have a long history as therapeutic tools in the treatment of human diseases and have been used as a source of medicines for ages. In search of new biologically active natural products, many plants and herbs used in traditional medicine are screened for natural products with pharmacological activity. In this paper, we present a group of natural products, the sesquiterpene coumarins isolated from plants, and describe their wide range of biological activity. Sesquiterpene coumarins are found in some plants of the families Apiaceae (Umbelliferae), Asteraceae (Compositae) and Rutaceae. The coumarin moiety is often umbelliferone (7-hydroxycoumarin) but scopoletin (7-hydroxy-6-methoxycoumarin) and isofraxidin (7-hydroxy-6,8-dimethoxycoumarin) are also found. These coumarins are linked to a C 15 terpene moiety through an ether linkage. Another group of sesquiterpene coumarins is the prenylated 4-hydroxycoumarins where the link between the coumarin and the C 15 terpene moiety is a C-C-bond at carbon 3 of the coumarin moiety. Finally, the prenyl-furocoumarintype sesquiterpenoids are a separate group of sesquiterpene coumarins based on the suggested biosynthetic pathway. Our relatively limited knowledge on the biosynthesis of sesquiterpene coumarins is reviewed.

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Simultaneous analysis of three bioactive compounds in Artemisia annua hairy root cultures by reversed‐phase high‐performance liquid chromatography–diode array detector

Abstract: The HPLC-PAD method developed for the simultaneous determination of three bioactive metabolites 1, 2 and 3 was simple, reproducible and sensitive.

Cited by 17 publications

References 24 publications

Effects of Chinese herbal mixture on performance, egg quality and blood biochemical parameters of laying hens

Effects of Chinese herbal mixture on performance, egg quality and blood biochemical parameters of laying hens

Bioassay-Guided Chromatographic Isolation and Identification of Antibacterial Compounds from Artemisia annua L. That Inhibit Clostridium perfringens Growth

Sesquiterpene coumarins

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