Simultaneous determination of four marker components in Yukmijihwang Tang by high performance liquid chromatography/diode array detector

Abstract

Simultaneous determination method of four marker components, paeoniflorin, loganin, 5-hydroxymethyl-2-furaldehyde and paeonol in traditional herbal medicine, Yukmijihwang tang, was developed to achieve rapidly and systematic quality control by high performance liquid chromatography coupled with diode array detection. To develop this high performance liquid chromatography method, C(18) column (5 microm, 120 A, 4.6 mm x 150 mm) was used with gradient elution of water and methanol as mobile phase. Validation of the chromatography method was evaluated by linearity, recovery, and precision test. Calibration curves of standard components showed excellent linearity with R(2) > 0.9990. Limits of detection and limits of quantification were in the ranges 0.01-0.02 and 0.04-0.07 microg/mL, respectively. The relative standard deviations of data of the intra-day and inter-day experiments were less than 1.15% and 3.76%, respectively. The results of recovery test were found to range from 94.88 to 107.43% with relative standard deviation values 0.07-2.66%. The results of validation suggested that this method was very accurate and stabilized.

Yukmijihwang-tang Inhibits Receptor Activator for Nuclear Factor-κB Ligand–Induced Osteoclast Differentiation

“…The four components were identified in the same retention time window as studied previously (data not shown). 19 The concentrations of paeoniflorin, paeonol, loganin, and 5-hydroxymethyl-2-furaldehyde in YMT were 0.67, 3.86, 0.67, and 3.89 lg/mg, respectively.…”

Optimization of the extraction process for the seven bioactive compounds in Yukmijihwang-tang, an herbal formula, using response surface methodology

“…[ 16 ] The main bioactive compounds of YJT are gallic acid, 5-hydroxymethylfurfural (5-HMF), morroniside, loganin, paeoniflorin, benzoic acid and paeonol which are analyzed using high performance liquid chromatography (HPLC)–ultraviolet–mass spectrometry, HPLC–diode array detector (DAD) or micellar electrokinetic chromatography. [ 17 18 19 20 ]…”

In vitro and in vivo evaluation of the genotoxicity of Gumiganghwal-tang, a traditional herbal prescription

“…Many traditional herbal medicines are used for the prevention and therapeutic treatment of diseases (Won et al, 2010). Indeed, many herbal therapies have become increasingly popular among patients and physicians, and they are now used by approximately 20% of the population in the United States because herbal medicines generally have few side effects and they are very effective (Bent, 2008).…”