Simultaneous Analysis of Hemoglobin Adducts of Acrylamide and Glycidamide by Gas Chromatography–Mass Spectrometry

Pérez, Hermes Licea; Cheong, Hae-Kwan; Yang, Jeong Sun; Osterman-Golkar, Siv

doi:10.1006/abio.1999.4267

Cited by 60 publications

(34 citation statements)

References 14 publications

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“…The assumption that GA is the genotoxic agent in AA exposure implies that it is important also to measure the ratio between internal doses of AA and GA. The data so far published indicate that the ratio between the adduct levels to N-terminal valine of GA and AA is about 1:1 [11,45], however with a lower value obtained by Licea-Perez et al [13].…”

mentioning

confidence: 71%

“…All previous data on the background level of the Hb adduct from AA [12][13][14][15][45][46], except for the study by Schettgen et al [47], came from the same laboratory as in the present study, using the same instruments and standards for quantification etc. In these published studies of altogether about 50 non-smoking individuals the adduct level varies between 0.02 and 0.07 nmol/g Hb.…”

Section: Discussionmentioning

confidence: 99%

“…Exposure to AA in work environments has been studied by measurements of stable reaction products (adducts) to hemoglobin (Hb), as a measure of internal dose [11][12][13][14][15]. In exposed workers dose-response relationships between Hb adducts and neurotoxic effects have been demonstrated [14,16].…”

Section: Introductionmentioning

confidence: 99%

“…Cancer of the large bowel, bladder 13 and kidney has been assessed in two Swedish case control studies, which were originally created for evaluating the carcinogenic effects of heterocyclic amines from diet [30,31].…”

mentioning

confidence: 99%

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Differences in hemoglobin adduct levels of acrylamide in the general population with respect to dietary intake, smoking habits and gender

Hagmar

Wirfält

Paulsson

et al. 2005

Mutation Research/Genetic Toxicology and Environmental Mutagene

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mentioning

confidence: 71%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

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Differences in hemoglobin adduct levels of acrylamide in the general population with respect to dietary intake, smoking habits and gender

Hagmar

Wirfält

Paulsson

et al. 2005

Mutation Research/Genetic Toxicology and Environmental Mutagene

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“…Hemoglobin adducts of acrylamide and glycidamide, its primary metabolite, were successfully developed as biomarkers of acrylamide exposure and used to investigate the health effects of this chemical on humans and animals (16)(17)(18)(19)(20). Assessing acrylamide exposure using these biomarkers provides comprehensive information about the amount of acrylamide that has entered a person's body over the previous 2 to 3 months, even when the actual exposure event has passed.…”

Section: Introductionmentioning

confidence: 99%

Assessment of the Relation between Biomarkers for Smoking and Biomarkers for Acrylamide Exposure in Humans

Vesper

Bernert

Ospina

et al. 2007

Cancer Epidemiology, Biomarkers &Amp; Prevention

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Smoking is an important source of acrylamide exposure in the general population. We assessed the relationship between hemoglobin adducts of acrylamide (HbAA) and glycidamide (HbGA) as biomarkers of acrylamide exposure and plasma cotinine (PC) as biomarkers of tobacco smoke exposure in 94 men and 67 women. The median (5th-95th percentile) biomarker concentrations (pmol/g Hb) in the group of individuals with PC concentrations of V10 ng/mL were 51 (29-155) and 34 (16-117) for HbAA and HbGA, respectively. They were significantly lower than those in the group of individuals with PC concentrations of >10 ng/mL [194 (87-403) and 107 (41-215) for HbAA and HbGA, respectively]. In individuals with PC concentrations of <1 ng/mL, HbAA and HbGA were similar to those observed in the group with PC values of V10 ng/mL. The intersubject variability was profoundly smaller in the group with PC values of V10 ng/mL compared with the group with PC values of >10 ng/mL. Although HbAA and HbGA could be categorized into distinguishable groups using PC concentration ranges commonly used to categorize presumed smokers and nonsmokers, no significant relationship was observed between these two biomarkers and PC within each group. The different exposure periods reflected by these biomarkers and the resulting different susceptibility to short-term variations in exposure patterns may in part explain these observations. The findings suggest that tobacco smoke exposure in individuals with PC values of <1 ng/mL has only a minimal effect

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Probing acrylamide alkylation sites in cysteine-free proteins by matrix-assisted laser desorption/ionisation time-of-flight

Bordini¹,

Hamdan²,

Righetti³

2000

Rapid Commun. Mass Spectrom.

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It is recognised that gel-separated proteins can experience a frequent modification provoked by the interaction of unpolymerized acrylamide monomers with the thiol group of cysteine to form a beta-cysteinyl-S-propionamide adduct. Other groups which have been implicated in this reaction include the hydroxyl group of tyrosine, the straightepsilon-amino group of lysine, and the free N-terminus. In a series of recent publications it has been demonstrated that at pH approximately 9.5 and in the presence of cysteine, none of these groups experienced measurable interaction with acrylamide monomers. To emphasise this conclusion we have used matrix-assisted laser desorption/ionisation with a reflectron time-of-flight mass spectrometer to examine a number of cysteine-free proteins incubated for various intervals with 30 mM acrylamide monomers at pH 9.5. These high resolution data suggest that, for short incubation times (>/=1 hour) and in the absence of cysteine, the straightepsilon-NH(2) group of lysine is the likely adduction site of acrylamide. Longer incubation times (>/=24 hours) with acrylamide monomers rendered the role of Cys as the favourite alkylation site less evident.

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Simultaneous Analysis of Hemoglobin Adducts of Acrylamide and Glycidamide by Gas Chromatography–Mass Spectrometry

Cited by 60 publications

References 14 publications

Differences in hemoglobin adduct levels of acrylamide in the general population with respect to dietary intake, smoking habits and gender

Differences in hemoglobin adduct levels of acrylamide in the general population with respect to dietary intake, smoking habits and gender

Assessment of the Relation between Biomarkers for Smoking and Biomarkers for Acrylamide Exposure in Humans

Probing acrylamide alkylation sites in cysteine-free proteins by matrix-assisted laser desorption/ionisation time-of-flight

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