Simultaneous Analysis of Hemoglobin Adducts of Acrylamide and Glycidamide by Gas Chromatography–Mass Spectrometry

Differences in hemoglobin adduct levels of acrylamide in the general population with respect to dietary intake, smoking habits and gender

“…The assumption that GA is the genotoxic agent in AA exposure implies that it is important also to measure the ratio between internal doses of AA and GA. The data so far published indicate that the ratio between the adduct levels to N-terminal valine of GA and AA is about 1:1 [11,45], however with a lower value obtained by Licea-Perez et al [13].…”

“…All previous data on the background level of the Hb adduct from AA [12][13][14][15][45][46], except for the study by Schettgen et al [47], came from the same laboratory as in the present study, using the same instruments and standards for quantification etc. In these published studies of altogether about 50 non-smoking individuals the adduct level varies between 0.02 and 0.07 nmol/g Hb.…”

Differences in hemoglobin adduct levels of acrylamide in the general population with respect to dietary intake, smoking habits and gender

“…The assumption that GA is the genotoxic agent in AA exposure implies that it is important also to measure the ratio between internal doses of AA and GA. The data so far published indicate that the ratio between the adduct levels to N-terminal valine of GA and AA is about 1:1 [11,45], however with a lower value obtained by Licea-Perez et al [13].…”

“…All previous data on the background level of the Hb adduct from AA [12][13][14][15][45][46], except for the study by Schettgen et al [47], came from the same laboratory as in the present study, using the same instruments and standards for quantification etc. In these published studies of altogether about 50 non-smoking individuals the adduct level varies between 0.02 and 0.07 nmol/g Hb.…”

A sensitive gas chromatographic–tandem mass spectrometric method for detection of alkylating agents in water: Application to acrylamide in drinking water, coffee and snuff

“…The characterization and quantification by GC-MS-MS of these derivatives have been described previously. 2,18 The calibration curves were linear in the range of concentrations tested and the regression coefficients (R 2 ) were > 0.999.…”

Probing acrylamide alkylation sites in cysteine-free proteins by matrix-assisted laser desorption/ionisation time-of-flight

“…The data of Pérez et al 8 indicated N ‐(2‐carbamoylethyl)valine as the main product of acrylamide adduction in vivo, in workers exposed for long periods to low levels of free acrylamide. Obviously, there is a clear difference between our in vitro data1,, 2 and the recent8 in vivo results. A possible reason for such a difference could be the pH of the environment in which such adduction reactions occur, in their case pH 7.4, in ours pH 9.5.…”

Differences in hemoglobin adduct levels of acrylamide in the general population with respect to dietary intake, smoking habits and gender

“…The assumption that GA is the genotoxic agent in AA exposure implies that it is important also to measure the ratio between internal doses of AA and GA. The data so far published indicate that the ratio between the adduct levels to N-terminal valine of GA and AA is about 1:1 [11,45], however with a lower value obtained by Licea-Perez et al [13].…”

“…All previous data on the background level of the Hb adduct from AA [12][13][14][15][45][46], except for the study by Schettgen et al [47], came from the same laboratory as in the present study, using the same instruments and standards for quantification etc. In these published studies of altogether about 50 non-smoking individuals the adduct level varies between 0.02 and 0.07 nmol/g Hb.…”

A sensitive gas chromatographic–tandem mass spectrometric method for detection of alkylating agents in water: Application to acrylamide in drinking water, coffee and snuff

“…The characterization and quantification by GC-MS-MS of these derivatives have been described previously. 2,18 The calibration curves were linear in the range of concentrations tested and the regression coefficients (R 2 ) were > 0.999.…”

Probing acrylamide alkylation sites in cysteine-free proteins by matrix-assisted laser desorption/ionisation time-of-flight

“…The data of Pérez et al 8 indicated N ‐(2‐carbamoylethyl)valine as the main product of acrylamide adduction in vivo, in workers exposed for long periods to low levels of free acrylamide. Obviously, there is a clear difference between our in vitro data1,, 2 and the recent8 in vivo results. A possible reason for such a difference could be the pH of the environment in which such adduction reactions occur, in their case pH 7.4, in ours pH 9.5.…”

Differences in hemoglobin adduct levels of acrylamide in the general population with respect to dietary intake, smoking habits and gender

“…The assumption that GA is the genotoxic agent in AA exposure implies that it is important also to measure the ratio between internal doses of AA and GA. The data so far published indicate that the ratio between the adduct levels to N-terminal valine of GA and AA is about 1:1 [11,45], however with a lower value obtained by Licea-Perez et al [13].…”

“…All previous data on the background level of the Hb adduct from AA [12][13][14][15][45][46], except for the study by Schettgen et al [47], came from the same laboratory as in the present study, using the same instruments and standards for quantification etc. In these published studies of altogether about 50 non-smoking individuals the adduct level varies between 0.02 and 0.07 nmol/g Hb.…”

A sensitive gas chromatographic–tandem mass spectrometric method for detection of alkylating agents in water: Application to acrylamide in drinking water, coffee and snuff

“…The characterization and quantification by GC-MS-MS of these derivatives have been described previously. 2,18 The calibration curves were linear in the range of concentrations tested and the regression coefficients (R 2 ) were > 0.999.…”

Probing acrylamide alkylation sites in cysteine-free proteins by matrix-assisted laser desorption/ionisation time-of-flight

“…The data of Pérez et al 8 indicated N ‐(2‐carbamoylethyl)valine as the main product of acrylamide adduction in vivo, in workers exposed for long periods to low levels of free acrylamide. Obviously, there is a clear difference between our in vitro data1,, 2 and the recent8 in vivo results. A possible reason for such a difference could be the pH of the environment in which such adduction reactions occur, in their case pH 7.4, in ours pH 9.5.…”