Simplified Approach to Identification of Aerobic Actinomycetes by Thin-Layer Chromatography

Staneck, Joseph L.; Roberts, Glenn D.

doi:10.1128/am.28.2.226-231.1974

Cited by 817 publications

(397 citation statements)

References 16 publications

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“…Biomass of A-T 0013 T used for chemotaxonomic analyses was obtained from a culture growing in trypticase soy medium (Difco) on a rotary shaker at 200 r.p.m., 28 ° C for 4 days. The isomer of diaminopimelic acid in the cell wall was determined using the method of Staneck and Roberts [31]. The acyl type in muramic acid of the peptidoglycan was determined using the method of Uchida and Aida [32].…”

Section: Chemotaxonomic Characterizationmentioning

confidence: 99%

Gordonia asplenii sp. nov., isolated from humic soil on bird’s nest fern (Asplenium nidus L.)

Suriyachadkun

Ngaemthao

Pujchakarn

et al. 2019

International Journal of Systematic and Evolutionary Microbiology

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A novel member of the actinobacteria, designated strain A-T 0013T, was isolated from humic soil on a bird’s nest fern (Asplenium nidus L.) collected from Khao Yai National Park in Thailand. According the results of a polyphasic taxonomic study, A-T 0013T had characteristics typical of members of the genus Gordonia . The 16S rRNA gene sequence indicated that A-T 0013T shared ≤98 % sequence similarity with all members of the genus Gordonia . The most closely related species was Gordonia effusa IFM 10200T (97.92 % sequence similarity). The average nucleotide identity based on blast (ANIb) value with G. effusa IFM 10200T was 76.81 %. The cell-wall peptidoglycan contained meso-diaminopimelic acid. The whole-cell sugars contained ribose, arabinose and galactose. The predominant menaquinone was MK-9(H2). The predominant fatty acids were C16 : 0, C18 : 1ω9c, summed feature 3 (C16 : 1ω7c/C16 : 1ω6c), and C18 : 0 10-methyl. Mycolic acid was present. The polar lipid profile for this strain ncluded diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and phosphatidylinositol mannosides. The G+C content of the genomic DNA was 66.1 mol%. Differentiation of A-T 0013T from the most closely related species, Gordonia effusa IFM 10200T, was evident from digital DNA–DNA hybridization values of 21.8 %. On the basis of the results of comparative analysis of phenotypic, chemotaxonomic and genotypic data, strain A-T 0013T is concluded to represent a novel species of the genus Gordonia , for which the name Gordonia asplenii sp. nov. is proposed. The type strain is A-T 0013T (=TBRC 11910T=NBRC 114549T).

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Section: Chemotaxonomic Characterizationmentioning

confidence: 99%

Gordonia asplenii sp. nov., isolated from humic soil on bird’s nest fern (Asplenium nidus L.)

Suriyachadkun

Ngaemthao

Pujchakarn

et al. 2019

International Journal of Systematic and Evolutionary Microbiology

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“…The isolate was examined for a broad range of phenotype properties known to be of value in Micromonospora systematics [ 10 , 16 ]. Standard chromatographic procedures were used to detect isomers of diaminopimelic acid [ 91 ], whole-organism sugars [ 92 ] and polar lipids [ 93 , 94 ], using freeze dried biomass harvested from yeast extract-malt extract broth cultures (International Streptomyces Project [ISP] medium 2) [ 95 ]. Similarly, cellular fatty acids extracted from the isolate were methylated and analyzed using the Sherlock Microbial Identification (MIDI) system and the resultant peaks identified using the ACTINO 6 database [ 96 ].…”

Section: Methodsmentioning

confidence: 99%

Biotechnological and Ecological Potential of Micromonospora provocatoris sp. nov., a Gifted Strain Isolated from the Challenger Deep of the Mariana Trench

et al. 2021

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A Micromonospora strain, isolate MT25T, was recovered from a sediment collected from the Challenger Deep of the Mariana Trench using a selective isolation procedure. The isolate produced two major metabolites, n-acetylglutaminyl glutamine amide and desferrioxamine B, the chemical structures of which were determined using 1D and 2D-NMR, including 1H-15N HSQC and 1H-15N HMBC 2D-NMR, as well as high resolution MS. A whole genome sequence of the strain showed the presence of ten natural product-biosynthetic gene clusters, including one responsible for the biosynthesis of desferrioxamine B. Whilst 16S rRNA gene sequence analyses showed that the isolate was most closely related to the type strain of Micromonospora chalcea, a whole genome sequence analysis revealed it to be most closely related to Micromonospora tulbaghiae 45142T. The two strains were distinguished using a combination of genomic and phenotypic features. Based on these data, it is proposed that strain MT25T (NCIMB 15245T, TISTR 2834T) be classified as Micromonospora provocatoris sp. nov. Analysis of the genome sequence of strain MT25T (genome size 6.1 Mbp) revealed genes predicted to responsible for its adaptation to extreme environmental conditions that prevail in deep-sea sediments.

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“…The fatty acid samples were prepared and analysed according to the instructions of the Sherlock Microbial Identification System (MIDI, version 6.0) [35]. Whole cell wall amino acid analysis mainly based on the methods of Staneck and Roberts [36] and Komagata and Suzuki [33].…”

Section: Physiology and Chemotaxonomymentioning

confidence: 99%

Flavimobilis rhizosphaerae sp. nov., isolated from rhizosphere soil of Spartina alterniflora

Cai

Shi

et al. 2021

International Journal of Systematic and Evolutionary Microbiology

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A novel Gram-stain positive, facultatively anaerobic, motile, irregularly rod-shaped bacterium, designated GY 10621T, was isolated from rhizosphere soil of Spartina alterniflora in Beihai City, Guangxi Province, PR China, and characterized using a polyphasic taxonomic approach. GY 10621T was positive for catalase and oxidase. Growth occurred at 4–42 °C (optimum 30–37 °C), at pH 5.0–9.0 (optimum pH 7.0) and in the presence of 0–5% NaCl (w/v) (optimum 1–3%). The main menaquinones were MK-9 (H4) (92.2 %) and MK-10 (7.8 %). The major cellular fatty acids were anteiso-C15 : 0 and C14 : 0. The peptidoglycan was the type A4α (l-Lys-Ser-d-Glu). The polar lipids included four phosphoglycolipids, four glycolipids, an unidentified lipid and six unidentified phospholipids. The DNA G+C content of the type strain was 71.7 mol%. On the basis of the results of 16S rRNA gene analysis, the type strain of a species with a validly published name with the highest similarity to GY 10621T was Flavimobilis soli KCTC 13155T (97.16 %), followed by Sanguibacter suarezii NBRC 16159T (96.39 %). The calculated results indicated that compared with GY 10621T, the average nucleotide identity (ANI) values of three strains closely related to GY 10621T (the two aforementioned type strains and ‘ S. massiliensis ’ Marseille-P3815) were 74.18–94.97 %, and the digital DNA–DNA hybridization (dDDH) values were 20.3–60.6 %. The results of 16S rRNA-based and genome-based phylogenetic tree analysis indicated that GY 10621T should be assigned to the genus Flavimobilis . On the basis of evidence from polyphasic studies, GY 10621T should be designated as representing a novel species of the genus Flavimobilis , for which the name Flavimobilis rhizosphaerae sp. nov. is proposed. The type strain is GY 10621T (=CGMCC 1.17411T=KCTC 49515T).

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Simplified Approach to Identification of Aerobic Actinomycetes by Thin-Layer Chromatography

Cited by 817 publications

References 16 publications

Gordonia asplenii sp. nov., isolated from humic soil on bird’s nest fern (Asplenium nidus L.)

Gordonia asplenii sp. nov., isolated from humic soil on bird’s nest fern (Asplenium nidus L.)

Biotechnological and Ecological Potential of Micromonospora provocatoris sp. nov., a Gifted Strain Isolated from the Challenger Deep of the Mariana Trench

Flavimobilis rhizosphaerae sp. nov., isolated from rhizosphere soil of Spartina alterniflora

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