Gordonia asplenii sp. nov., isolated from humic soil on bird’s nest fern (Asplenium nidus L.)

Abstract

A novel member of the actinobacteria, designated strain A-T 0013T, was isolated from humic soil on a bird’s nest fern (Asplenium nidus L.) collected from Khao Yai National Park in Thailand. According the results of a polyphasic taxonomic study, A-T 0013T had characteristics typical of members of the genus Gordonia . The 16S rRNA gene sequence indicated that A-T 0013T shared ≤98 % sequence similarity with all members of the genus Gordonia . The most closely related species was Gordonia effusa IFM 10200T (97.92 % sequence similarity). The average nucleotide identity based on blast (ANIb) value with G. effusa IFM 10200T was 76.81 %. The cell-wall peptidoglycan contained meso-diaminopimelic acid. The whole-cell sugars contained ribose, arabinose and galactose. The predominant menaquinone was MK-9(H2). The predominant fatty acids were C16 : 0, C18 : 1ω9c, summed feature 3 (C16 : 1ω7c/C16 : 1ω6c), and C18 : 0 10-methyl. Mycolic acid was present. The polar lipid profile for this strain ncluded diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and phosphatidylinositol mannosides. The G+C content of the genomic DNA was 66.1 mol%. Differentiation of A-T 0013T from the most closely related species, Gordonia effusa IFM 10200T, was evident from digital DNA–DNA hybridization values of 21.8 %. On the basis of the results of comparative analysis of phenotypic, chemotaxonomic and genotypic data, strain A-T 0013T is concluded to represent a novel species of the genus Gordonia , for which the name Gordonia asplenii sp. nov. is proposed. The type strain is A-T 0013T (=TBRC 11910T=NBRC 114549T).

Gordonia pseudamarae sp. nov., a home for novel actinobacteria isolated from stable foams on activated sludge wastewater treatment plants

“…S3). In general, the chemotaxonomic properties of this isolate were in good agreement with those of Gordonia strains [1,14,54], including those classified previously as N. amarae [7,8]. To this end, isolate CON9 T produced meso-A 2 pm as the diamino acid of the peptidoglycan, arabinose, galactose and ribose as whole-organism sugars, and a polar lipid pattern composed of diphosphatidylglycerol, phosphatidylethanolamine, phoshphatidylinositol and phosphatidylinositol mannosides (Fig.…”

“…S3). In general, the chemotaxonomic properties of this isolate were in good agreement with those of Gordonia strains [1, 14, 54], including those classified previously as N. amarae [7, 8].…”

Gordonia tangerina sp. nov., isolated from seawater

“…Members in the genus were isolated from diverse matrices. Most of them were isolated from sputum samples from patients with pulmonary disease [2][3][4][5][6], soil and sediment [7][8][9][10][11][12][13][14][15][16][17][18][19][20], sludge and wastewater bioreactor from wastewater treatment plants [21][22][23][24][25][26][27][28][29], and animals [30,31]. Gordonia species have a great capacity to degrade hydrocarbons, recalcitrant pollutants and natural compounds that are hard to biodegrade [32], such as oil [33], phthalic acid esters [34], polycyclic aromatic hydrocarbons [35] and rubber [36].…”