Silver Nitrate Increases Embryo Production in Anther Culture of Brussels Sprouts

Biddington, N. L.; Sutherland, R. A.; Robinson, Helen T.

doi:10.1093/oxfordjournals.aob.a087649

Cited by 62 publications

(19 citation statements)

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“…This poor regeneration capacity appears to reflect the initial poor growth of a particular callus type. Biddington et al [3] found that certain genotypes of Brussels sprout responded well to anther culture by producing a large number of embryos even in the absence of AgNO3. They suggested that these lines may produce lower levels of endogenous ethylene or be less sensitive to it.…”

Section: Discussionmentioning

confidence: 99%

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Effect of silver nitrate on long-term culture and regeneration of callus from Brassica oleracea var. gemmifera

Williams¹,

Pink²,

Biddington³

1990

Plant Cell Tiss Organ Cult

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Incorporation of AgNO 3 (1-10mg 1 -l) into the culture medium of Brassica oleracea var. gemmifera callus significantly improved growth and allowed long-term callus culture. In the absence of AgNO3, callus died shortly after removal from the hypocotyl explants. Regeneration of shoots from callus on low-hormone medium was also enhanced by AgNO 3 . Significant differences in shoot production were found between the three genotypes examined. Cv. Aries produced large numbers of shoots even in the absence of AgNO3.

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Section: Discussionmentioning

confidence: 99%

“…It has also proved useful for enhancing embryo yield from anther culture in Brassica oleracea var. gemmifera [3].…”

Section: Introductionmentioning

confidence: 99%

Effect of silver nitrate on long-term culture and regeneration of callus from Brassica oleracea var. gemmifera

Williams¹,

Pink²,

Biddington³

1990

Plant Cell Tiss Organ Cult

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“…Also, modification of culture medium components can increase the number of embryoid and green regenerated plantlets in different species. The addition of silver nitrate, an ethylene inhibitor, in anther culture media has improved embryo production in wheat (Lashermes 1992) and in Brussels sprouts (Biddington et al 1988;Ockendon & Clenagham 1993). Silver nitrate may promote pollen embryogenesis by blocking the inhibitory effect of endogenous ethylene on the embryo (Purnhauser et al 1987).…”

Section: Introductionmentioning

confidence: 99%

The effects of silver nitrate, colchicine, cupric sulfate and genotype on the production of embryoids from anthers of tetraploid wheat (Triticum turgidum)

Ghaemi

Sarrafi

Alibert

1994

Plant Cell Tiss Organ Cult

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Anther culture of four tetraploid wheat (Triticum turgidum) genotypes was studied using ten different culture medium treatments in a randomized block design with four replicates. Each replicate consisted of 2 pots with 3 plants. Anther donor plants were grown in a greenhouse with a 16 h day / 8 h night at 25°C and 15°C, respectively. The first treatment which was considered as the control, was potato 2 medium modified by adding 0.5 g 1-1 glutamine and solidified by gelrite (4 g 1-1). The nine test treatments differed from the control by addition of 3 different concentrations of silver nitrate (1, 2.5 and 5 mg l-l), colchicine (10, 100 and 200 mg 1 -l) or cupric sulfate (2, 5 and 10 mg 1-1). The study of about 2000 anthers per genotype and treatment showed that both genotype and treatment affected embryoid formation. The presence of cupric sulfate (10 mg 1-1) and silver nitrate (2.5 and 5 mg 1-1) usually increased the frequency of embryoid formation in 3 genotypes out of the 4 studied. On the contrary, colchicine had a significant and negative effect on anther culture responses for three out of the four genotypes studied. Because of the large genotype x medium interaction, it is very difficult to identify the best medium for embryo production by all genotypes studied.

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“…Brassica alboglabra (19,22), Brassica napus and Brassica oleracea (33) have been well developed. Recently, the use of EIs promoted embryo production from anther culture (2) and shoot differentiation from callus culture (26) Ethylene evolved from explants grown on medium with or without AgNO3 or AVG was measured. Before measurement, the serum stopper of each flask in which explants were grown was removed.…”

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Role of Ethylene on de Novo Shoot Regeneration from Cotyledonary Explants of Brassica campestris ssp. pekinensis (Lour) Olsson in Vitro

Chi¹,

Pua²,

Goh³

1991

Plant Physiol.

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The promotive effect of AgNO3 and aminoethoxyvinylglycine (AVG) on in vitro shoot regeneration from cotyledons of Brasska campestris ssp. peklnensls in relation to endogenous 1-aminocyclopropane-1-carboxylic acid (ACC) synthase, ACC, and ethylene production was investigated. AgNO3 enhanced ACC synthase activity and ACC accumulation, which reached a maximum after 3 to 7 days of culture. ACC accumulation was concomitant with increased emanation of ethylene which peaked after 14 days. In contrast, AVG was inhibitory to endogenous ACC synthase activity and reduced ACC and ethylene production. The promotive effect of AVG on shoot regeneration was reversed by 2-chloroethylphosphonic acid at 50 micromolar or higher concentrations, whereas explants grown on AgNO3 medium were less affected by 2-chloroethylphosphonic acid. The distinctive effect of AgNO3 and AVG on endogenous ACC synthase, ACC, and ethylene production and its possible mechanisms are discussed.Plants usually exhibit enhanced ethylene production when wounded or under environmental stresses or pathogen attack (31). Ethylene biosynthesis in plants occurs via SAM' and ACC intermediates using methionine as a precursor (31), but the molecular mechanism of its action is not clear. It has been known for some time that cultured plant cells evolve ethylene (1 1 Cotyledonary explants excised from 3-d-old aseptically germinated seedlings of Brassica campestris ssp. pekinensis cv Shantung and cv Wong Bok were used. Because our preliminary investigations showed that explants cultured in 50-mL Erlenmyer flasks and 100-x 25-mm Petri dishes exhibited the similar capacity of shoot regeneration, both culture containers were used in this study. Explants were cultured either in a flask containing 25 mL medium or in a Petri dish containing 30 mL medium consisting of all constituents of Murashige and Skoog's medium (16)

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Silver Nitrate Increases Embryo Production in Anther Culture of Brussels Sprouts

Cited by 62 publications

References 0 publications

Effect of silver nitrate on long-term culture and regeneration of callus from Brassica oleracea var. gemmifera

Effect of silver nitrate on long-term culture and regeneration of callus from Brassica oleracea var. gemmifera

The effects of silver nitrate, colchicine, cupric sulfate and genotype on the production of embryoids from anthers of tetraploid wheat (Triticum turgidum)

Role of Ethylene on de Novo Shoot Regeneration from Cotyledonary Explants of Brassica campestris ssp. pekinensis (Lour) Olsson in Vitro

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