Effect of silver nitrate on long-term culture and regeneration of callus from Brassica oleracea var. gemmifera

Williams, Jessica; Pink, David; Biddington, N. L.

doi:10.1007/bf00034493

Cited by 45 publications

(15 citation statements)

References 15 publications

(17 reference statements)

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“…This has also been observed by Ozcan et al (1992). The concentration of silver nitrate used in this study (1 uM) was lower than that reported to have a positive influence on regeneration in other Block, 1988;Chi & Pua, 1989;Chi et al, 1990;Williams et al, 1990), but raising the concentration to 10 uM totally inhibited rooting in our experiments (results not shown). Inhibition of root regeneration was at its most evident after one month on rooting medium, as the rooting frequency (67%) obtained for shoots from shoot culture medium without silver nitrate was reduced to 26% when silver nitrate was filter sterilized into the medium (Table 2 treatments 2 and 5).…”

Section: Resultscontrasting

confidence: 71%

Regeneration of pea (Pisum Sativuml.) by the thin cell layer nodal system: Influence of explant culture media on rooting and plantlet formation

Madsen¹,

Nauerby

Frederiksen

et al. 1998

Acta Agriculturae Scandinavica, Section B — Soil & Plant Sc

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Regeneration of pea (Pisum sativum L.) by the thin cell layer nodal system: influence of explant culture media on rooting and plantlet formation. Accepted April 28, 1998. Acta Agric. Scand., Sect. B, Soil and Plant Sci. 48: 58-64, 1998. © 1998 Scandinavian University Press.The effect of different shoot culture compositions on the thin cell layer nodal (TCLn) regeneration system for pea was tested. By varying the composition of the shoot culture medium, up to 86% of the regenerated shoots in cultivar Terese were able to produce roots at standard rooting conditions. Addition of silver nitrate to the shoot culture medium decreased vitrification, but greatly reduced rooting frequencies There was no major influence on shoot regeneration or rooting capacity when using 1.5% or 3.0% glucose or sucrose The use of 5 × 15 -6 M indole butyric acid in the rooting medium did not increase rooting. Seed-derived progeny of regenerates contained the normal diploid number of chromosomes (2n = 14). It was demonstrated that rooting of shoots arising from TCLn explants was influenced more by the composition of the regeneration medium than by the rooting medium. These results imply a rather long-lasting effect of the components in the regeneration medium.

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Section: Resultscontrasting

confidence: 71%

Regeneration of pea (Pisum Sativuml.) by the thin cell layer nodal system: Influence of explant culture media on rooting and plantlet formation

Madsen¹,

Nauerby

Frederiksen

et al. 1998

Acta Agriculturae Scandinavica, Section B — Soil & Plant Sc

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“…However, even recalcitrant B. rapa and other species have been triggered to develop shoots by applying ethylene inhibitors like aminoethoxyvinylglycine and silver nitrate (Chi et al 1990, Williams et al 1990, Sethi et al 1990, Palmer 1992, Pua and Chi 1993. Some transformation protocols claim the inclusion of silver nitrate to be obligatory for suflicient shoot regeneration , Pental et al 1993, particularly when kanamycin is used as a selectable marker (Mukhopadhyay et al 1992).…”

Section: Tissue Culture and Plant Regenerationmentioning

confidence: 99%

Genetic transformation of Brassica

Poulsen

1996

Plant Breeding

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Tissue culture regeneration of plants from Brassica species can be achieved from various explant types. Their performance, however, varies with the genotype and age of the explant and is sensitive to medium supplements and Agrobacterium cocultivation. Most Brassica transformations were carried out using Agrobacterium and it was established that nopaline and agropine strains of .4. tumefaciens and A. rhizogenes, respectively, are the most efficient. Application of virulence gene induction is ambiguous. Binary vectors are mostly apphed in spite of indications that cointegrate vectors are more efficient. Cotransformation is successful with two different bacteria or with one bacterium carrying both plasmids, however, it has proved problematic to integrate the two transgenes into separate loci. Transformations with wild type rol genes develop hairy roots, from which transgenic plants can be regenerated. Vectorless transformation is feasible with microprojectile bombardment of microspore derived embryos being the most promising.Antibiotic markers providing resistance to kanamycin, hygromycin, and spectinomycin are applicable as selectable markers, while chloramphenicol is not suitable. Among herbicides, phosphinotricin gives good results while chlorsulfuron is unsuitable for in vitro selection. Major therapeutic antibiotics being successfully applied are carbenicillin, cefotaxime, amoxycillin, and ticarcillin. Standard methods for evaluating transgenic plants also have been applied to Brassica species. Some breeding objectives obtained through genetic transformation are presented.

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“…On the other hand, the inhibitory effect of ethylene on shoot formation has been altered by inhibitors of either ethylene action or ethylene production. Thus, incorporation of AgNO 3 in the culture medium promoted shoot regeneration from callus of Brassica oleracea (Williams et al 1990). Also, in some other members of the Cruciferae family addition of the ethylene inhibitors AgNO 3 or amino-ethyoxyvinyl-glycine (AVG) in the culture medium enhanced shoot regeneration from seedling explants (Pua et al 1990).…”

Section: Introductionmentioning

confidence: 99%

Promotion of petunia (Petunia hybrida L.) regeneration in vitro by ethylene

Dimasi-Theriou

Economou

Sfakiotakis

1993

Plant Cell Tiss Organ Cult

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The influence of ethylene on shoot and root formation from petunia leaf explants was studied in cultures in test tubes placed in 5 1 glass jars. Reduction of the endogenously produced ethylene by inclusion of ethysorb (KMnO4) , an ethylene absorbent, caused a decrease of the number of shoots. On the other hand, supplementing the cultures with ethylene (0.01-10 ppm) caused a marked increase of the number of shoots without, however, any effect on the length and fresh weight. Ethylene treatments (1 ppm) were found to be most effective when they were applied in the second week of culturing of petunia explants. Addition of Co ++ to the medium resulted in a reduction of the endogenously produced ethylene and concomitantly reduced shoot formation. Similarly, inclusion of Ag +, an inhibitor of ethylene action, resulted in poor shoot formation. Ethylene also appeared to play a role on rooting of petunia microshoots in vitro in an auxin-free medium. Ethylene at a concentration of 10 ppm induced adventitious root formation considerably, whereas at low levels (0.01-1ppm) it had no influence on rooting.

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Effect of silver nitrate on long-term culture and regeneration of callus from Brassica oleracea var. gemmifera

Cited by 45 publications

References 15 publications

Regeneration of pea (Pisum Sativuml.) by the thin cell layer nodal system: Influence of explant culture media on rooting and plantlet formation

Regeneration of pea (Pisum Sativuml.) by the thin cell layer nodal system: Influence of explant culture media on rooting and plantlet formation

Genetic transformation of Brassica

Promotion of petunia (Petunia hybrida L.) regeneration in vitro by ethylene

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