Silver nanoparticle modified surfaces induce differentiation of mouse kidney-derived stem cells

Abstract

In this paper, we interrogate the influence of silver nanoparticle (AgNPs)-based model surfaces on mouse kidney-derived stem cells (mKSCs) differentiation. The widespread use of silver in biomedical and consumer products requires understanding of this element's effect on kidney cells. Moreover, the potential for using stem cells in drug discovery require methods to direct their differentiation to specialized cells. Hence, we generated coated model substrates containing different concentrations of surface immobilized AgNPs, and used them to evaluate properties and functions of mKSCs. Initially, mKSCs exhibited reduced viability on higher silver containing surfaces. However, longer culture periods assisted mKSCs to recover. Greater degree of cell spreading and arborization led by AgNPs, suggest podocyte differentiation. Proximal tubule cell marker's expression revealed differentiation to the specific lineage. Although the exact mechanism underpinning these findings require significant future efforts, this study demonstrate silver's capacity to stimulate mKSC differentiation, which may provide opportunities for drug screenings.

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“…The synthetic protocols which we developed involve a range of reduction and capping agents (lipids, 2-mercaptosuccinic acid, citrate, polyvinyl-sulphonate, etc. ), including a procedure that benefited from the properties of natural products, such as cacao extract [64,71,[73][74][75][76][77][78]83,86]. These nanoparticles can be immobilised electrostatically or covalently to plasma polymer films containing appropriate surface functionalities ( Figure 5) [70,71,75,79,87].…”

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“…[132][133][134] Studies involving stem cells demonstrated that plasma polymer coatings containing silver nanoparticles enhanced the adipogenic capacity of human bonemarrow-derived mesenchymal stem cells, [137] while mouse kidney-derived stem cell differentiation was directed towards podocyte lineages. [138] These studies indicate the need for research beyond the simple cytotoxicity assays conducted in most published work. The capacity of silver-containing plasma polymer coatings for regulating physiological processes also provides opportunities that could be explored by developing novel pathways to precisely control the rate of release and local concentration of silver ions and nanoparticles.…”

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“…Proximal tubular cell markers showed differentiation of a specific lineage. It was concluded that AgNPs stimulate mKSCs differentiation [31].…”