Silver decorated green nanocolloids as potent antibacterial and antibiofilm agent against antibiotic resistant organisms isolated from tannery effluent

Appl Biochem Biotechnol

Priya

Veerasami

et al. 2021

Mastitis is a widespread disease in dairy cattle occurring throughout the world. The increased use of antibiotics brings about the development of antibiotic-resistant microbes. The application of antibiotics in dairy farming led to increased antibiotic resistance and represents a major obstacle for the treatment of mastitis. Recent advancements in nanotechnology led to the development of nanocolloids to overcome disadvantages posed by conventional antimicrobial agents. Hence, a novel, environmentally friendly, cost-effective, biocompatible, and long-term antibacterial represents a promising solution for medicine and farming. Hence, polyherbal nanocolloids (PHNc) was formulated by using the extracts of Syzygium aromaticum, Cinnamomum verum, Emblica officinalis, Terminalia belerica, Terminalia chebula, and Cymbopogon citratus and physicochemically characterized. From mastitis milk samples, microorganisms were isolated including Acinetobacter junii, Klebsiella pneumoniae, Pseudomonas stutzeri, and Acinetobacter baumannii and screened for antibiotic susceptibility. All the isolated strains were tested with PHNc and compared with antibiotics. Minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), and biofilm assays were performed at different concentrations, and antibacterial effects were quantified. In our results, PHNc showed potent bacteriostatic, bactericidal, and antibiofilm activity against all the strains. Our results indicated that PHNc can reduce the virulence factors responsible for infection by different bacterial strains. This study confirmed that PHNc had the potential to inhibit the growth of pathogenic Gram-negative and Gram-positive strains and could be utilized as an alternative to antibiotics to inhibit multidrug-resistant microbial pathogens in cattle.

Section: Discussionmentioning

confidence: 99%

Section: Formulation Of Polyherbal Nanocolloids (Phnc)mentioning

confidence: 99%

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Novel Polyherbal Nanocolloids to Control Bovine Mastitis

Appl Biochem Biotechnol

Priya

Veerasami

et al. 2021

“…The solution was then boiled in the hot plate for 20-30 min. The solution is then cooled and allowed to settle down followed by ltration using Whatmann No 1 lter paper and stored at 4ºC for further use [15][16][17][18]. The myrobalans extract was subjected to qualitative phytochemical analysis [19] and quantitative carbohydrate analysis using Anthrone method [20].…”

Section: Preparation Of Triphala Extract Of and Qualitative Phytocompounds Analysismentioning

confidence: 99%

Myrobalans Mediated Nanocolloids in Controlling Marine Pathogens

Parthasarathy

Rameshkumar

et al. 2021

Preprint

Aquaculture production is affected by disease outbreak, which affect the production, profitability, and sustainability of the global aquaculture industry. Antibiotics have been widely used to control various pathogens. Due to its uncontrollable and indiscriminate usage, pathogens have developed resistance towards antibiotics. This current study aims to synthesize green silver- of silver nanocolloids (MBNc) from the extract of three myrobalans. MBNc was characterized by using UV-Vis spectroscopy, DLS, Zeta potential, FE-SEM. EDX, HRTEM, and SAED techniques. The array of antimicrobial assays were performed to evaluate the efficacy of MBNc against vibriosis causing pathogens including V. harveyi, V. alginolyticus, V. parahaemolyticus, and foodborne pathogen S. haemolyticus, isolated from infected fish. Further, the presence of ESBL genes including CTX-M-15 and Amp C were analyzed in control and MBNc treated strains. From our studies, it was observed that MBNc was very effective in controlling the growth and biofilm formation in all tested marine pathogens and effectively abolish the genes encoding ESBL CTX-M-15 in antibiotic-resistant pathogens tested. Thus, MBNc can be formulated to control the growth of marine pathogens and it can be used as an alternative to antibiotics to prevent infection in cage culturing and aquafarming.

“…This helps us to identify the possible biomolecules, which could play the role in reduction, stabilization and capping during synthesis of myconanoparticles. (Perkin Elmer Spectrum100 FT-IR) (20)(21)(22)(23). Colloidal nanoparticle solution was used to identify the hydrodynamic size (Z average), polydispersity index (PDI), and surface charge (zeta potential) of the synthesized nanoparticles using particle size analyser combined with zeta analyser (Malvern Instruments Ltd, UK).…”

Section: Physio-chemical Characterization Of Green Silver Nanoparticlesmentioning

confidence: 99%

Silver Decorated Myconanoparticles Control Growth and Biofilm Formation in Uropathogenic E. coli

Appl Biochem Biotechnol

Kathun

Hemalatha

2021

Nanotechnology involves the synthesis of nanoparticles that has been used in therapeutic application for treating diseases. In this present study we have adopted the synthesis of myconanoparticles from the extracellular extract of endophytic fungi Penicillium sclerotiorum (PsNps) and validated for its antibacterial potential against antibiotic resistant uropathogenic E. coli and ATCC (25922) strain of E. coli. Endophytic fungi were isolated from the healthy leaves of Tamarindus indica. The genomic DNA from endophytic fungi was isolated and ITS region was ampli ed by polymerase chain reaction (PCR) using universal fungal primers ITS1 and ITS4and sequenced for the identi cation of endophytic fungal isolates. Penicillium sclerotiorum extract were used for the synthesis silver nanoparticles (PsNps) and were characterized by UV-Vis spectroscopy, Fourier transform-infrared spectroscopy (FTIR), Zeta potential, FE-SEM and Energy-Dispersive X-ray analysis (EDAX). Antibacterial activity of PsNps was tested against the antibiotic resistant uropathogenic E. coli and ATCC (25922) strain of E. coli. Further experiments were carried out to explore the potential of PsNps in regulating the CTX-M-15 gene. The antimicrobial activity showed that the PsNps inhibited growth, bio lm formation in both the strains of E. coli. The expression of gene encoding CTX-M-15 was down regulated in resistant strain of uropathogenic E. coli. Our results suggest that the PsNps could be used as an alternative source for the antibiotics. Thus, further studies can be conducted to prove the in vivo potential of PsNps and can be formulated for commercialization.