Silver Decorated Myconanoparticles Control Growth and Biofilm Formation in Uropathogenic E. coli

Abstract

Nanotechnology involves the synthesis of nanoparticles that has been used in therapeutic application for treating diseases. In this present study we have adopted the synthesis of myconanoparticles from the extracellular extract of endophytic fungi Penicillium sclerotiorum (PsNps) and validated for its antibacterial potential against antibiotic resistant uropathogenic E. coli and ATCC (25922) strain of E. coli. Endophytic fungi were isolated from the healthy leaves of Tamarindus indica. The genomic DNA from endophytic fungi was isolated and ITS region was ampli ed by polymerase chain reaction (PCR) using universal fungal primers ITS1 and ITS4and sequenced for the identi cation of endophytic fungal isolates. Penicillium sclerotiorum extract were used for the synthesis silver nanoparticles (PsNps) and were characterized by UV-Vis spectroscopy, Fourier transform-infrared spectroscopy (FTIR), Zeta potential, FE-SEM and Energy-Dispersive X-ray analysis (EDAX). Antibacterial activity of PsNps was tested against the antibiotic resistant uropathogenic E. coli and ATCC (25922) strain of E. coli. Further experiments were carried out to explore the potential of PsNps in regulating the CTX-M-15 gene. The antimicrobial activity showed that the PsNps inhibited growth, bio lm formation in both the strains of E. coli. The expression of gene encoding CTX-M-15 was down regulated in resistant strain of uropathogenic E. coli. Our results suggest that the PsNps could be used as an alternative source for the antibiotics. Thus, further studies can be conducted to prove the in vivo potential of PsNps and can be formulated for commercialization.

The Effects of L-Lysine-α-oxidase Enzyme and Trichoderma harzianum Rifai Culture Liquid on the Formation of Biofilms by Uropathogenic Multiresistant E. coli

“…Secondly, the presence of many adhesion factors that contribute to the rapid colonization of the urinary tract [13,14]. Thirdly, UPEC actively forms biofilms on various biogenic and abiogenic surfaces [8,15,16] and, as a result, is difficult to treat, which makes it difficult to eliminate the pathogen and leads to chronic or recurrent infections. It is known that the mechanism of biofilm formation by microorganisms is regulated by QS molecules and depends on environmental factors, availability of nutrients, hydrodynamic conditions, etc.…”