Side by Side Comparison of Chemical Compounds Generated by Aqueous Pretreatments of Maize Stover, Miscanthus and Sugarcane Bagasse

Gómez, Leonardo D.; Vanholme, Ruben; Bird, Susannah; Goeminne, Geert; Trindade, Luisa M.; Polikarpov, Igor; Simister, Rachael; Morreel, Kris; Boerjan, Wout; McQueen‐Mason, Simon J.

doi:10.1007/s12155-014-9480-2

Cited by 20 publications

(17 citation statements)

References 51 publications

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“…This could be explained by lignin extraction from the inner regions of the cell wall, and subsequent condensations and redeposition on the surface as reported for wood samples [54]. In comparison with the results from conventional thermo-chemical pretreatment of Miscanthus (180 °C, 40 min) by Gomez et al, and similar amount of lignin is removed by 0.2 M NaOH pretreatment, whereas more lignin (210-240 mg/g biomass) is presented in the biomass after water and 0.2 M H 2 SO 4 pretreatments [50]. Under our conditions, the distribution and structure of lignin could be changed under microwave assisted acidic conditions, and the results show a decreasing lignin amount as measured by using acetyl bromide methods.…”

Section: Effect Of Microwave Assisted Pretreatment On Different Biomasupporting

confidence: 71%

“…Haverty et al studied peroxide/formic acid assisted pre-treament for Miscanthus under autothermal conditions, and the results showed 0.3-4.37 % cellulose removal across conditions assayed [49]. One of our co-author, Gomez et al studied conventional thermo-chemical pretreatment for Miscanthus material (20-180 °C, holding time 40 min), and their results shows 6-12 mg reducing sugar release/mg biomass (yield from total carbohydrate: 1.88-3.76 %) when temperature is 180 °C [50]. The reducing sugar yield in this work is 19 times higher within half the time than the result from Gomez et al In comparison with other pretreatment methods, our microwave assisted For Miscanthus, microwave assisted pretreatment is therefore more efficient in releasing reducing sugars during pretreatment; the reason could be its unique heating mechanism (magnitude of heating depends on the dielectric properties of the subject) leading to more efficient biomass decomposition.…”

Section: Monosaccharides Analysis In the Pretreatment Mediamentioning

confidence: 99%

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Microwave assisted chemical pretreatment of Miscanthus under different temperature regimes

Zhu

Macquarrie

Simister

et al. 2015

Sustain Chem Process

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Background: Miscanthus is a major bioenergy crop in Europe and a potential feedstock for second generation biofuels. The most efficient and realistic method to produce fermentable sugars from lignocellulosic biomass is by enzymatic hydrolysis, assisted by thermo-chemical pretreatment. Recently, microwave technology has drawn growing attention, because of its unique effects and performance on biomass. Result: In this work, microwave energy was applied to facilitate NaOH and H 2 SO 4 pretreatment for Miscanthus under different temperatures (130-200 °C) for 20 min. The yields of reducing sugars from Miscanthus during the pretreatment process increased up to 180 °C and then declined with increasing temperature. Out results here showed a remarkable sugar yield from available carbohydrate (73 %) at the temperature of 180 °C by using 0.2 M H 2 SO 4. In comparison with conventional heating pretreatment studied at same temperature with same biomass material, the reducing sugar release in this study was 17 times higher within half the time. It was highlighted that the major sugar component could be tuned by changing pretreatment temperature or pretreatment media. Optimally, the glucose and xylose yield from available carbohydrate are 47 and 22 % by using 0.2 M H 2 SO 4 and NaOH respectively when temperature was 180 °C. The digestibility of pretreated Miscanthus was 10 times higher than that of untreated biomass. 68-86 % of the lignin content was removed from biomass by 0.2 M NaOH. Simultaneous saccharification fermentation (SSF) results showed an ethanol production of 143-152 mg/g biomass by using H 2 SO 4 /NaOH microwave assisted pretreatment, which is 7 times higher than that of untreated Miscanthus. Biomass morphology was studied by SEM, showing temperature has a strong influence on lignin removal process, as different lignin deposits were observed. At the temperature of 180 °C, NaOH pretreated biomass presented highly exposed fibres, which is a very important biomass characteristic for improved enzymatic hydrolysis. Conclusion: Compared to conventional pretreatment, microwave assisted pretreatment is more energy efficient and faster, due to its unique heating mechanism leading to direct interaction between the polar part of biomass and electromagnetic field. The results of this work present promising potential for using microwave to assist biomass thermo-chemical pretreatment.

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Section: Effect Of Microwave Assisted Pretreatment On Different Biomasupporting

confidence: 71%

Section: Monosaccharides Analysis In the Pretreatment Mediamentioning

confidence: 99%

Microwave assisted chemical pretreatment of Miscanthus under different temperature regimes

Zhu

Macquarrie

Simister

et al. 2015

Sustain Chem Process

Self Cite

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“…Consequently, the alkaline pretreatment results in partial extraction of the lignin from the biomass. The acid pretreatment used, on the other hand, hydrolyzes hemicellulosic glycosidic bonds (Gómez et al, 2014). As (di)ferulates link hemicelluloses with lignin, this acid pretreatment also will solubilize part of lignin.…”

Section: Discussionmentioning

confidence: 99%

Silencing CHALCONE SYNTHASE in Maize Impedes the Incorporation of Tricin into Lignin and Increases Lignin Content

et al. 2016

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Lignin is a phenolic heteropolymer that is deposited in secondary-thickened cell walls, where it provides mechanical strength. A recent structural characterization of cell walls from monocot species showed that the flavone tricin is part of the native lignin polymer, where it is hypothesized to initiate lignin chains. In this study, we investigated the consequences of altered tricin levels on lignin structure and cell wall recalcitrance by phenolic profiling, nuclear magnetic resonance, and saccharification assays of the naturally silenced maize (Zea mays) C2-Idf (inhibitor diffuse) mutant, defective in the CHALCONE SYNTHASE Colorless2 (C2) gene. We show that the C2-Idf mutant produces highly reduced levels of apigenin-and tricin-related flavonoids, resulting in a strongly reduced incorporation of tricin into the lignin polymer. Moreover, the lignin was enriched in b-b and b-5 units, lending support to the contention that tricin acts to initiate lignin chains and that, in the absence of tricin, more monolignol dimerization reactions occur. In addition, the C2-Idf mutation resulted in strikingly higher Klason lignin levels in the leaves. As a consequence, the leaves of C2-Idf mutants had significantly reduced saccharification efficiencies compared with those of control plants. These findings are instructive for lignin engineering strategies to improve biomass processing and biochemical production.

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“…Gomez et al studied conventional heating pretreatment of maize stover. Their results showed that substantial quantities of sugars were released from the biomass by using NaOH or H 2 SO 4 , particularly from hemicellulose fraction [42]. Fig.…”

Section: The Effect Of H 2 Somentioning

confidence: 94%

Efficient sugar production from sugarcane bagasse by microwave assisted acid and alkali pretreatment

Zhu

Rezende

Simister

et al. 2016

Biomass and Bioenergy

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131

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Sugarcane bagasse represents one of the best potential feedstocks for the production of second generation bioethanol. The most efficient method to produce fermentable sugars is by enzymatic hydrolysis, assisted by thermochemical pretreatments. Previous research was focused on conventional heating pretreatment and the pretreated biomass residue characteristics. In this work, microwave energy is applied to facilitate sodium hydroxide (NaOH) and sulphuric acid (H 2 SO 4) pretreatments on sugarcane bagasse and the efficiency of sugar production was evaluated on the soluble sugars released during pretreatment. The results show that microwave assisted pretreatment was more efficient than conventional heating pretreatment and it gave rise to 4 times higher reducing sugar release by using 5.7 times less pretreatment time. It is highlighted that enrichment of xylose and glucose can be tuned by changing pretreatment media (NaOH/H 2 SO 4) and holding time. SEM study shows significant delignification effect of NaOH pretreatment, suggesting a possible improved enzymatic hydrolysis process. However, severe acid conditions should be avoided (long holding time or high acid concentration) under microwave heating conditions. It led to biomass carbonization, reducing sugar production and forming 'humins'. Overall, in comparison with conventional pretreatment, microwave assisted pretreatment removed significant amount of hemicellulose and lignin and led to high amount of sugar production during pretreatment process, suggesting microwave heating pretreatment is an effective and efficient pretreatment method.

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Side by Side Comparison of Chemical Compounds Generated by Aqueous Pretreatments of Maize Stover, Miscanthus and Sugarcane Bagasse

Cited by 20 publications

References 51 publications

Microwave assisted chemical pretreatment of Miscanthus under different temperature regimes

Microwave assisted chemical pretreatment of Miscanthus under different temperature regimes

Silencing CHALCONE SYNTHASE in Maize Impedes the Incorporation of Tricin into Lignin and Increases Lignin Content

Efficient sugar production from sugarcane bagasse by microwave assisted acid and alkali pretreatment

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