Short communication: Supplementing lysine and methionine in a lactation diet containing a high concentration of wet corn gluten feed did not alter milk protein yield

Mullins, C.R.; Weber, D. W.; Block, E.; Smith, James B.; Brouk, Michael J.; Bradford, B.J.

doi:10.3168/jds.2013-6755

Cited by 10 publications

(6 citation statements)

References 29 publications

(31 reference statements)

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“…We observed that supplying RPM plus RPL numerically improved milk yield more than RPM alone. Inconsistent with our results, RPM plus RPL failed to improve milk yield of dairy cows (Trináctý et al 2009; Mullins et al 2013; Lee et al 2015). Several factors can contribute to this inconsistency including different diets (barley vs corn based), basal Met and Lys percentages of MP and ratio, level and type of supplemental AA, level of milk production, etc.…”

Section: Discussionsupporting

confidence: 87%

Rumen-protected methionine and lysine: effects on milk production and plasma amino acids of dairy cows with reference to metabolisable protein status

Awawdeh

2016

Journal of Dairy Research

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Two experiments were conducted to study the effects of rumen-protected Met (RPM) alone or with rumen-protected Lys (RPL) on milk yield and plasma amino acids of dairy cows. In experiment 1, 24 multiparous Holstein cows (154 DIM) were assigned to one of 3 groups where each cow received 0 g/d of RPM and RPL (C), 30 g/d of RPM (M), or 30 g/d of RPM plus 25 g of RPL (ML). The study lasted for 8 weeks where milk yield and composition were determined weekly. Daily milk yield averaged 28·0, 27·8, and 29·7 kg/cow for the C, M, and ML groups, respectively. Dietary treatments had no effects (P ≥ 0·54) on milk contents of fat, lactose, solid non-fat or total solids. Milk protein content in the ML group was greater (P < 0·05) than the C and M groups. Plasma levels of all AA were not significantly (P ≥ 0·09) affected by supplemental RPL and/or RPM. In experiment 2, 30 multiparous Holstein cows (100 DIM) were assigned to one of 3 groups where each cow received 0 g/d of RPM and RPL (C), 50 g/d of RPM (M), or 50 g/d of RPM plus 25 g/d of RPL (ML). The study lasted for 5 weeks. Cows in the M (30·5 kg) and ML (31·4 kg) groups produced (P < 0·05) more milk than those of the C group (29·1 kg). Under conditions of this study, RPM plus RPL improved milk yield and protein contents of dairy cows and was better than supplying RPM alone. Response in milk yield to RPM and RPL was affected by the MP status of cows which deserves further investigation.

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Section: Discussionsupporting

confidence: 87%

Rumen-protected methionine and lysine: effects on milk production and plasma amino acids of dairy cows with reference to metabolisable protein status

Awawdeh

2016

Journal of Dairy Research

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“…In the current experiment, there were no observed effects of level of RP-Lys on MUN, although the values were similar or lower than the values observed in other studies (Swanepoel et al, 2010;Mullins et al, 2013;Weiss, 2019) suggesting N supply was adequate but not excessive or severely imbalanced. There was no effect of treatment on efficiency parameters.…”

Section: Discussionsupporting

confidence: 78%

“…In many parts of the United States, rations for dairy cows contain corn and corn-based products. Corn milling by-products are an economical source of NE L and MP, but can lead to limitations in Lys supply due to the predominance of zein protein, a poor source of Lys (Coleman and Larkins, 1999;Mullins et al, 2013;Weiss, 2019). Thus, when corn and corn products comprise a significant proportion of the diets, dairy nutritionists have formulated rations using rumen-protected sources of Lys to meet Lys requirements for high-producing cows.…”

Section: Introductionmentioning

confidence: 99%

Effects of increased doses of lysine in a rumen-protected form on plasma amino acid concentration and lactational performance of dairy cows fed a lysine-deficient diet

Malacco

Beckett

Hilger

et al. 2022

Journal of Dairy Science

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The objective of these studies was to determine the effects of feeding a novel rumen-protected Lys (RP-Lys) product on plasma AA, lactational performance, and Lys bioavailability. To evaluate RP-Lys on lactation performance a corn-based diet (42.5% of corn silage and 21.9% of corn and corn by-products, on DM basis) was formulated to be Lys deficient but adequate in Met, energy, and metabolizable protein. Thirty-six lactating Holstein cows were fed either a Lys-deficient control diet (CON) with no added RP-Lys, or diets containing 0.3% of RP-Lys (0.3RP-Lys) or 0.6% of RP-Lys (0.6RP-Lys) for 8 wk. There were no effects on dry matter intake (mean ± SD; 26.1 ± 0.58 kg/d), milk yield (37.9 ± 0.72 kg/d), or milk composition to the RP-Lys supplementation. No effect was observed on plasma AA concentrations except for His. Plasma His was linearly reduced by Lys feeding (42.6, 41.2, 30.0 ± 4.09 μM, for CON, 0.3RP-Lys, and 0.6RP-Lys, respectively). Calculated efficiency of Lys utilization decreased linearly with RP-Lys supplementation. In the companion study, 3 rumen-cannulated lactating dairy cows were used in a 3 × 3 Latin square design to assess the bioavailability of the RP-Lys. Free Lys (HCl-Lys), RP-Lys, and water were administered separately by postruminal bolus dosing. The Lys bioavailability was assessed by the ratio of area under the curve of Lys plasma concentration for RP-Lys compared with HCl-Lys and discounted for the area under the curve for water bolus dose. The estimated bioavailability of the RP-Lys was 24.4% ± 4.61. In summary, increased supplemental doses of Lys had no effect on Lys plasma concentration and lactational performance when fed to dairy cows on a corn-based diet, although altered Lys as % of essential AA was observed. However, the lack of effects should be considered in light of the lower-thanexpected bioavailability of the RP-Lys.

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“…The increase in milk protein percentage resulted in an increase in milk protein yield over cows fed CMP (P < 0.05). In another experiment with a diet that contained 27% corn gluten feed, supplemental RP-Lys and RP-Met (different products than used in the current experiment) did not affect milk protein yield or concentrations (Mullins et al, 2013). The control diet in that study contained an estimated 6.3% Lys in the MP, whereas the CMP diet only contained 6.0% (Table 2).…”

Section: Production Measuresmentioning

confidence: 64%

Effects of feeding diets composed of corn silage and a corn milling product with and without supplemental lysine and methionine to dairy cows

Weiss

2019

Journal of Dairy Science

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Formulating diets with high inclusion rates of a feed that provides necessary nutrients at lower-thanmarket prices for those nutrients should increase income over feed costs if the feed is not detrimental to yields of milk and milk components. The objective of this study was to determine whether cows fed a diet composed of approximately 53% corn silage, 44% corn milling product (68% dry matter, 21% crude protein, 37% neutral detergent fiber, and 9% starch) and 3% minerals (CMP) would have similar productivity as cows fed a control diet of predominantly corn silage, alfalfa silage, corn grain, and soybean meal. Based on the National Research Council ( 2001) dairy model, the CMP diet was inadequate in metabolizable methionine and extremely low in metabolizable lysine. A third treatment (CMP+AA) was the same as the CMP diet but was supplemented with rumen-protected lysine and methionine. Twenty-one Holstein cows were used in a replicated Latin square (28-d periods) design to evaluate production responses to the 3 diets. Diets were formulated to contain the same concentration of net energy for lactation and metabolizable protein (MP) based on the National Research Council model, but diets with CMP contained more neutral detergent fiber (38.3 vs. 31.4%) and less starch (21.6 vs. 30.5%) than the control diet. Lysine as a percent of MP was 6.5, 6.0, and 6.8 for the control, CMP, and CMP+AA diets, respectively, and methionine was 1.8, 1.8, and 2.3% of MP, respectively. Dry matter intake was not affected by diet (24.3 kg/d), but milk yield was lower for cows fed either CMP diet than for those fed control (36.0 vs 38.1 kg/d). Milk fat concentrations were normal and not affected by diet (3.7%). Milk protein concentration was greater for cows fed CMP+AA than for cows fed the other 2 treatments (3.19 vs. 3.11%); however, milk protein yield was greatest for cows fed the conventional diet. The concentration of methionine in plasma was significantly greater for cows fed CMP+AA than for cows fed the other diets. Plasma lysine concentrations were greater for cows fed the conventional diet than for those fed the other 2 diets. Plasma concentrations of several essential AA were lower for cows fed either CMP diet. Based on calculated energy balance, diets contained similar concentrations of net energy for lactation, but cows fed CMP diets partitioned more energy toward body energy reserves than did control cows, perhaps because supply of specific AA limited milk synthesis.

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Short communication: Supplementing lysine and methionine in a lactation diet containing a high concentration of wet corn gluten feed did not alter milk protein yield

Cited by 10 publications

References 29 publications

Rumen-protected methionine and lysine: effects on milk production and plasma amino acids of dairy cows with reference to metabolisable protein status

Rumen-protected methionine and lysine: effects on milk production and plasma amino acids of dairy cows with reference to metabolisable protein status

Effects of increased doses of lysine in a rumen-protected form on plasma amino acid concentration and lactational performance of dairy cows fed a lysine-deficient diet

Effects of feeding diets composed of corn silage and a corn milling product with and without supplemental lysine and methionine to dairy cows

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