Short Communication Genetic variation in a wild population of the ‘sleep’ passion fruit (Passiflora setacea) based on molecular markers

Cerqueira-Silva, Carlos Bernard Moreno; Santos, Elisa Susilene Lisboa dos; Conceição, L. D. H. C. S. Da; Cardoso-Silva, Cláudio Benício; Pereira, A. S.; Oliveira, A. C. B. de; Corrêa, Ronan Xavier

doi:10.4238/2012.march.22.3

Cited by 11 publications

(13 citation statements)

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“…However, an intraspecific analysis with ISSR markers found higher polymorphism (98%) in P. alata . In interspecific analyses, a higher rate of polymorphism in combination with an increased average number of amplified bands per primer is indicative of high genetic variability (Cerqueira-Silva et al, 2012). Nevertheless, high intraspecific genetic variability was also observed here among the eight P. cincinnata accessions.…”

Section: Discussionsupporting

confidence: 54%

“…In agreement with this expectation, we observed greater interspecific polymorphism (99%) than intraspecific polymorphism (76%) in Passiflora. An intraspecific analysis performed using RAPD markers reported an average of 63.8% polymorphism in P. nitida (Ganga et al, 2004) and in P. setacae (Cerqueira-Silva et al, 2012). However, an intraspecific analysis with ISSR markers found higher polymorphism (98%) in P. alata .…”

Section: Discussionmentioning

confidence: 94%

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ISSR markers in wild species of Passiflora L. (Passifloraceae) as a tool for taxon selection in ornamental breeding

et al. 2015

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ABSTRACT. Inter simple sequence repeat (ISSR) molecular markers were developed and used to investigate interspecific genetic variation in 25 wild species of Passiflora preserved in an active germplasm bank (BAG-Passifloras); intraspecific diversity was also analyzed in P. cincinnata accessions. Of 31 primers tested, 20 identified polymorphic loci with a total of 331 bands, suggesting high polymorphism in the sample. Interspecific polymorphism was greater than intraspecific polymorphism. This is a common finding in studies of genetic variation using dominant markers. The ISSRs revealed species-specific amplification bands in 11 species; these bands ranged from 200 to 1000 bp, and they will be of use for developing SCAR markers for the identification of germplasm in further studies. The use of Jaccard's similarity coefficient to obtain a dendrogram by the UPGMA clustering method distributed the taxa into five major groups, with differences among grouping with respect to 18535 ISSR application in wild Passiflora ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (4): 18534-18545 (2015) principal coordinate analysis. Despite the high cophenetic correlation coefficient (r = 0.94) of the dendrogram, taxonomic inconsistencies were observed; similar irregularities have been reported previously in studies using dominant markers. Intraspecific analysis of P. cincinnata accessions revealed a larger genetic distance between those from Bahia (P2) and from Minas Gerais (P2), indicating that both accessions have considerable potential as parents in a genetic improvement program for this species.

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Section: Discussionsupporting

confidence: 54%

Section: Discussionmentioning

confidence: 94%

ISSR markers in wild species of Passiflora L. (Passifloraceae) as a tool for taxon selection in ornamental breeding

et al. 2015

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“…From the total number of markers, 82% were polymorphic in RAPD, and 91.2% were polymorphic in ISSR, showing the highgenetic variability among the six cultivars. A high polymorphism indicating the high-genetic variability of the Passiflora genus by RAPD markers has already been reported by Bellon et al (2009Bellon et al ( , 2014, Viana et al (2010), Castro et al (2011), andCerqueira-Silva et al (2012). This fact have been verified also by Santos et al (2011b), Costa et al (2012), and Sousa et al (2015) by using ISSR markers.…”

Section: Descriptor Number 22)supporting

confidence: 63%

Morphoagronomic and molecular characterization of ornamental passion fruit cultivars

Fonseca

Faleiro

Junqueira

et al. 2017

Pesq. agropec. bras.

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-The objective of this work was to validate the morphoagronomic descriptors used in the protection processes of plant cultivars in Brazil, by characterizing six cultivars of ornamental passion fruit. The BRS Rubiflora, BRS Rosea Púrpura, BRS Céu do Cerrado, BRS Roseflora, BRS Estrela do Cerrado, and BRS Pérola do Cerrado cultivars, 33 morphoagronomic descriptors, and two molecular markers were used. The categorical morphoagronomic descriptors were analyzed by frequency distribution and multivariate analyses. The quantitative morphoagronomic descriptors were subjected to the analysis of variance and to the comparison of the means of each cultivar. The random amplified polymorphic DNA (RAPD) and the inter-simple sequence repeats (ISSR) markers were used for molecular analysis. A high-validation rate was observed for the morphoagronomic descriptors used in the protection of plant cultivars. The analyses of variance showed significant differences between the quantitative descriptors, and the molecular markers confirmed the genetic differences among the cultivars. There was a high correlation between the calculated distances based on the categorical morphoagronomic descriptors and molecular markers. The morphoagronomic descriptors and molecular markers are useful and complementary for the characterization and differentiation of cultivars.Index terms: Passiflora, molecular markers, multivariate analysis, plant cultivar protection. Caracterização morfoagronômica e molecular de cultivares de maracujazeiro ornamentalResumo -O objetivo deste trabalho foi validar os descritores morfoagronômicos utilizados nos processos de proteção de cultivares no Brasil, por meio da caracterização de seis cultivares de maracujazeiro ornamental. As cultivares BRS Rubiflora, BRS Rosea Púrpura, BRS Céu do Cerrado, BRS Roseflora, BRS Estrela do Cerrado e BRS Pérola do Cerrado, 33 descritores morfoagronômicos e dois marcadores moleculares foram utilizados. Os descritores morfoagronômicos categóricos foram analisados por meio da distribuição de frequência e análises multivariadas. Os descritores morfoagronômicos quantitativos foram submetidos à análises de variância e à comparação entre médias das cultivares. Os marcadores "random amplified polymorphic DNA" (RAPD) e "inter-simple sequence repeats" (ISSR) foram usados para análise molecular. Observou-se alta taxa de validação dos descritores morfoagronômicos utilizados na proteção de cultivares. As análises de variância mostraram diferenças significativas entre os descritores quantitativos das cultivares, e os marcadores moleculares confirmaram as diferenças genéticas entre elas. Houve alta correlação entre as distâncias calculadas com base nos descritores morfoagronômicos categóricos e nos marcadores moleculares. Os descritores morfoagronômicos e os marcadores moleculares são úteis e complementares para a caracterização e a diferenciação das cultivares.Termos para indexação: Passiflora, marcadores moleculares, análise multivariada, proteção de cultivares.

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“…For some species, such as P. caerulea , P. cincinnata , P. coccinea , and P. setacea , interspecific hybrids are being developed and characterized [33,54,72] and estimates of genetic diversity and confirmation of the hybrids based on molecular markers are being carried out [54,62,63]. …”

Section: Contributions Of Molecular and Genetic Studies For The Chmentioning

confidence: 99%

“…Approximately 90% of the area designated for passion fruit culture is cultivated with P. edulis , and P. alata is the second most cultivated species of the genus [54,61]. Although they represent a small proportion of national production, the cultivation and consumption of fruits from wild species, such as Passiflora cincinnata , Passiflora nitida , P. quadrangularis , and P. setacea , have been reported in the literature [31,57,61,62,63]. …”

Section: Introductionmentioning

confidence: 99%

Genetic Breeding and Diversity of the Genus Passiflora: Progress and Perspectives in Molecular and Genetic Studies

Cerqueira-Silva

Jesus

Santos

et al. 2014

IJMS

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Despite the ecological and economic importance of passion fruit (Passiflora spp.), molecular markers have only recently been utilized in genetic studies of this genus. In addition, both basic genetic researches related to population studies and pre-breeding programs of passion fruit remain scarce for most Passiflora species. Considering the number of Passiflora species and the increasing use of these species as a resource for ornamental, medicinal, and food purposes, the aims of this review are the following: (i) to present the current condition of the passion fruit crop; (ii) to quantify the applications and effects of using molecular markers in studies of Passiflora; (iii) to present the contributions of genetic engineering for passion fruit culture; and (iv) to discuss the progress and perspectives of this research. Thus, the present review aims to summarize and discuss the relationship between historical and current progress on the culture, breeding, and molecular genetics of passion fruit.

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Short Communication Genetic variation in a wild population of the ‘sleep’ passion fruit (Passiflora setacea) based on molecular markers

Cited by 11 publications

References 10 publications

ISSR markers in wild species of Passiflora L. (Passifloraceae) as a tool for taxon selection in ornamental breeding

ISSR markers in wild species of Passiflora L. (Passifloraceae) as a tool for taxon selection in ornamental breeding

Morphoagronomic and molecular characterization of ornamental passion fruit cultivars

Genetic Breeding and Diversity of the Genus Passiflora: Progress and Perspectives in Molecular and Genetic Studies

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