ISSR markers in wild species of Passiflora L. (Passifloraceae) as a tool for taxon selection in ornamental breeding

Abstract

ABSTRACT. Inter simple sequence repeat (ISSR) molecular markers were developed and used to investigate interspecific genetic variation in 25 wild species of Passiflora preserved in an active germplasm bank (BAG-Passifloras); intraspecific diversity was also analyzed in P. cincinnata accessions. Of 31 primers tested, 20 identified polymorphic loci with a total of 331 bands, suggesting high polymorphism in the sample. Interspecific polymorphism was greater than intraspecific polymorphism. This is a common finding in studies of genetic variation using dominant markers. The ISSRs revealed species-specific amplification bands in 11 species; these bands ranged from 200 to 1000 bp, and they will be of use for developing SCAR markers for the identification of germplasm in further studies. The use of Jaccard's similarity coefficient to obtain a dendrogram by the UPGMA clustering method distributed the taxa into five major groups, with differences among grouping with respect to 18535 ISSR application in wild Passiflora ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (4): 18534-18545 (2015) principal coordinate analysis. Despite the high cophenetic correlation coefficient (r = 0.94) of the dendrogram, taxonomic inconsistencies were observed; similar irregularities have been reported previously in studies using dominant markers. Intraspecific analysis of P. cincinnata accessions revealed a larger genetic distance between those from Bahia (P2) and from Minas Gerais (P2), indicating that both accessions have considerable potential as parents in a genetic improvement program for this species.

Morphoagronomic and molecular characterization of ornamental passion fruit cultivars

“…A high polymorphism indicating the high-genetic variability of the Passiflora genus by RAPD markers has already been reported by Bellon et al (2009Bellon et al ( , 2014, Viana et al (2010), Castro et al (2011), andCerqueira-Silva et al (2012). This fact have been verified also by Santos et al (2011b), Costa et al (2012), and Sousa et al (2015) by using ISSR markers.…”

Diversity and genetic stability in banana genotypes in a breeding program using inter simple sequence repeats (ISSR) markers

“…Plants with lower levels of genetic similarity, as determined by molecular markers, could be used as the parent material in breeding programs for the production of hybrids (Sousa et al, 2015).…”

Characterization of the genetic diversity in Passiflora spp. in the Boyacá Department, Colombia

“…The genetic diversity in Passiflora has been studied with different molecular techniques, such as random amplified polymorphic DNA (RAPD), amplified fragment length polymorphism (AFLP), and sequence-related amplified polymorphism (SRAP) (Oluoch et al, 2018;Vieira et al, 2019); however, simple sequence repeat (SSR) markers are the most used techniques for the characterization of germplasm in different crops since they are highly polymorphic, reproducible and co-dominant, with wide genome coverage (Gramazio et al, 2018). Among the microsatellite markers, inter-simple sequence repeat (ISSR), which combines characteristics of the RAPD and microsatellites techniques, facilitates multilocus genomic analysis with a single primer based on regions of SSR, presents good reproducibility, has a low cost, is simple, does not need previous genome information (Morillo et al, 2018), and has proven to be polymorphic and informative in studies on genetic diversity in Passiflora (Santos et al, 2011;Sousa et al, 2015;Batista et al, 2017;Oliveira et al, 2019;Vianna et al, 2019).…”