Serological studies of Corynebacterium kutscheri and coryneform bacteria using an enzyme-linked immunosorbent assay (ELISA)

Abstract

SummaryAn enzyme-linked immunosorbent assay [ELISA) to measure Corynebacterium kutscberi antibodies in mice and rats was developed. Seven C. kutscberi isolates showed considerable serological relationship, but Japanese isolates differed from the British isolates. The ELISA appeared specific since C. kutscberi antigen did not react with antisera against 8 heterologous coryneform species. Antibodies to C. kutscberi were to a limited extent absorbed by autologous and homologous antigen, but not at all by the heterologous coryneform species.In naturally infected wild Rattus norvegicus and laboratory NA rats, the ELISA demonstrated high ODs to C. kutscheri.

Biology and Diseases of Mice

“…Agglutination serology is available, and immunofluorescence, immunodiffusion, and ELISA tests have been reported (Boot et al, 1995a). Agglutination serology is available, and immunofluorescence, immunodiffusion, and ELISA tests have been reported (Boot et al, 1995a).…”

Rat bite fever

“…It can not be decided whether this is a reflection of the geography or the host species. Geographically related differences have been observed among rodent pathogenic Corynebacterium kutscheri strains (Boot et al, 1995) and other rodent pathogens such as Pasteurella pneumotropica show host species related differences in bacterial properties (Boot et al, 1993a).…”

Biology and Diseases of Mice

“…Additionally, organisms compatible with C. kutscheri are usually demonstrable with tissue Gram stains on lesions from clinically apparent cases. Agglutination serology is available, and immunofluorescence, immunodiffusion, and ELISA tests have been reported (Boot et al, 1995b).…”