Serological and molecular investigation of porcine sapovirus infection in piglets in Xinjiang, China

Qiao, Jun; Tian, Lulu; Meng, Qingling; Zhang, Xingxing; Haiting, Lu; Gong, Shasha; Zibing, Cheng; Xue-peng, Cai; Zhang, Jinsheng; Zhang, Zaichao; Kuojun, Cai; Chen, Chuangfu

doi:10.1007/s11250-016-1023-8

Cited by 8 publications

(6 citation statements)

References 28 publications

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“…PoSaV was detected in 7.6% of the samples. On the other hand, the lowest PoSaV detection rates were observed in China, where the virus was detected in 3.42% of 146 diarrheic stool samples of one-month-old piglets [ 84 ].…”

Section: Norovirus and Sapovirusmentioning

confidence: 99%

Epidemiology of Astrovirus, Norovirus and Sapovirus in Greek pig farms indicates high prevalence of Mamastrovirus suggesting the potential need for systematic surveillance

et al. 2022

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Backround Astrovirus, Norovirus and Sapovirus exhibit a wide distribution in swine pig herds worldwide. However, the association of porcine Astrovirus (PAstV), porcine Norovirus (PoNoV) and porcine Sapovirus (PoSaV) with disease in pigs remains uncertain. In this study, we investigated the prevalence of PAstV, PoNoV and PoSaV in Greek pig farms using both conventional RT-PCR and SYBR-Green Real-time RT-PCR in an effort to compare the sensitivity of the two methods. We examined 1400 stool samples of asymptomatic pigs originating from 28 swine farms throughout Greece in pools of five. Results PAstV was detected in all 28 swine farms examined, with an overall prevalence of 267/280 positive pools (95.4%). Porcine Caliciviruses prevalence was found at 36 and 57 out of the 280 examined samples, by the conventional and SYBR-Green Real time RT-PCR, respectively. Sequencing and phylogenetic analysis of the positive samples revealed that the detected PAstV sequences are clustered within PAstV1, 3 and 4 lineages, with PAstV3 being the predominant haplotype (91.2%). Interestingly, sequencing of the Calicivirus positive samples demonstrated the presence of non-target viruses, i.e. Sapovirus, Kobuvirus and Sapelovirus sequences and one sequence highly similar to bat Astrovirus, while no Norovirus sequence was detected. Conclusions The high prevalence of PAstV in Greek pig farms poses a necessity for further investigation of the pathogenicity of this virus and its inclusion in surveillance programs in case that it proves to be important. To our knowledge, this is the first epidemiological study of these viruses in pig farms in Greece.

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Section: Norovirus and Sapovirusmentioning

confidence: 99%

Epidemiology of Astrovirus, Norovirus and Sapovirus in Greek pig farms indicates high prevalence of Mamastrovirus suggesting the potential need for systematic surveillance

et al. 2022

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“…Only GIII SaVs have been adapted to cell culture, so the attempts to isolate other SaVs in cell culture for diagnostic purposes are not practical. Antibodies against porcine SaVs could be detected in the SaV-infected pig serum samples using GIII SaV-specific VP1-ELISA (Jun et al, 2016;Liu et al, 2012b;Liu et al, 2014a) or recombinant porcine SaV viral-like particle ELISA (Alcalá et al, 2010;Lu et al, 2016). However, the sensitivity of the above assays is lower than the detection methods targeting viral nucleic acids (Oka et al, 2015).…”

Section: Diagnosismentioning

confidence: 99%

Porcine sapoviruses: Pathogenesis, epidemiology, genetic diversity, and diagnosis

et al. 2020

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The first porcine Sapovirus (SaV) Cowden strain was discovered in 1980. To date, eight genogroups (GIII, V-IX) and three genogroups (GIII, GV, and GVI) of porcine SaVs have been detected from domestic pigs worldwide and wild boars in Japan, respectively based on the capsid sequences. Although GIII Cowden strain replicated in the villous epithelial cells and caused intestinal lesions in the proximal small intestines (mainly in duodenal and less in jejunum), leading to mild to severe diarrhea, in the orally inoculated neonatal gnotobiotic pigs, the significance of porcine SaVs in different ages of pigs with diarrhea in the field is still undetermined. This is due to two reasons: 1) similar prevalence of porcine SaVs was detected in diarrheic and non-diarrheic pigs; and 2) coinfection of porcine SaVs with other enteric pathogens is common in pigs. Diagnosis of porcine SaV infection is mainly based on the detection of viral nucleic acids using reverse transcription (RT)-PCR and sequencing. Much is unknown about these genetically diverse viruses to understand their role in pig health and to evaluate whether vaccines are needed to prevent SaV infection.

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“…Sapovirus has a wide host spectrum, infecting not only humans ( 6 , 7 ), but also oysters ( 8 ), bats ( 9 ), rats ( 10 ), dogs and cats ( 11 , 12 ), chimpanzees ( 13 ), and pigs ( 14 ). SaV is one of the main pathogens that cause acute gastroenteritis in children and the elderly ( 1 , 15 , 16 ) and was observed for the first time under electron microscopy from the stool samples of children with diarrhea from United Kingdom in 1976 ( 17 , 18 ).…”

Section: Introductionmentioning

confidence: 99%

High Genetic Diversity of Porcine Sapovirus From Diarrheic Piglets in Yunnan Province, China

et al. 2022

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As one of the most important enteric viruses, sapovirus (SaV) can infect humans and a variety of animals. Until now, 19 SaV genogroups have been identified, among which 4 from human (GI, GII, GIV, and GV) and 8 from swine (GIII, GV–GXI). Porcine sapovirus (PoSaV) GIII has been prevalent in China; however, the status of PoSaV infection in Yunnan province remains unknown. In this study, 202 fecal samples were collected from piglets associated with outbreaks of acute diarrhea in Yunnan between January and May 2020. PoSaV detection revealed that the total PoSaV infection rate in Yunnan was 35.2%, with 21 PoSaV strains determined and phylogenetically analyzed. The phylogenetic tree analyses demonstrated that twenty PoSaV strains belonged to GIII and fell into five genotypes, whereas one PoSaV strain (YNQB) belonged to GV. Sequence alignments revealed deletions in VP2 region in 10 of the 20 GIII strains, as well as deletions and insertions in VP1 region of the GV strain (YNQB). Furthermore, genomic recombination analyses showed that two GIII strains (YNAN and YNJD) were recombinants, closely related to reference sequences MK965898 and LC215880, MK965898 and FJ387164, respectively. In summary, PoSaV-GIII strains were identified in Yunnan in 2020, and for the first time, a PoSaV-GV strain was identified from China, whereas the comprehensive analyses illustrated high genetic diversity of Yunnan PoSaV strains. This study may shed new light on the current PoSaV infections in Yunnan and pave the way toward further control of the PoSaV infections in China.

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Serological and molecular investigation of porcine sapovirus infection in piglets in Xinjiang, China

Cited by 8 publications

References 28 publications

Epidemiology of Astrovirus, Norovirus and Sapovirus in Greek pig farms indicates high prevalence of Mamastrovirus suggesting the potential need for systematic surveillance

Epidemiology of Astrovirus, Norovirus and Sapovirus in Greek pig farms indicates high prevalence of Mamastrovirus suggesting the potential need for systematic surveillance

Porcine sapoviruses: Pathogenesis, epidemiology, genetic diversity, and diagnosis

High Genetic Diversity of Porcine Sapovirus From Diarrheic Piglets in Yunnan Province, China

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