We report a new search for weakly interacting massive particles (WIMPs) using the combined low background data sets acquired in 2016 and 2017 from the PandaX-II experiment in China. The latest data set contains a new exposure of 77.1 live days, with the background reduced to a level of 0.8×10^{-3}  evt/kg/day, improved by a factor of 2.5 in comparison to the previous run in 2016. No excess events are found above the expected background. With a total exposure of 5.4×10^{4}  kg day, the most stringent upper limit on the spin-independent WIMP-nucleon cross section is set for a WIMP with mass larger than 100  GeV/c^{2}, with the lowest 90% C.L. exclusion at 8.6×10^{-47}  cm^{2} at 40  GeV/c^{2}.

We report the WIMP dark matter search results using the first physics-run data of the PandaX-II 500 kg liquid xenon dual-phase time-projection chamber, operating at the China JinPing underground Laboratory. No dark matter candidate is identified above background. In combination with the data set during the commissioning run, with a total exposure of 3.3×10 4 kg-day, the most stringent limit to the spin-independent interaction between the ordinary and WIMP dark matter is set for a range of dark matter mass between 5 and 1000 GeV/c 2 . The best upper limit on the scattering cross section is found 2.5 × 10 −46 cm 2 for the WIMP mass 40 GeV/c 2 at 90% confidence level.Weakly interacting massive particles, WIMPs in short, are a class of hypothetical particles that came into existence shortly after the Big Bang. The WIMPs could naturally explain the astronomical and cosmological evidences of dark matter in the Universe. The weak interactions between WIMPs and ordinary matter could lead to the recoils of atomic nuclei that produce detectable signals in deep-underground direct detection experiments. Over the past decade, the dual-phase xenon time-projection chambers (TPC) emerged as a powerful technology for WIMP searches both in scaling up the target mass, as well as in improving background rejection [1][2][3]. LUX, a dark matter search experiment with a 250 kg liquid xenon target, has recently reported the best limit of 6×10 −46 cm 2 on the WIMP-nucleon scattering cross section [4] The PandaX-II experiment, a half-ton scale dual-phase xenon experiment at the China JinPing underground Laboratory (CJPL), has recently reported the dark matter search results from its commissioning run (Run 8,19.1 live days) with a 5845 kg-day exposure [5]. The data were contaminated with significant 85 Kr background. After a krypton distillation campaign in early 2016, PandaX-II commenced physics data taking in March 2016. In this paper, we report the combined WIMP search results using the data from the first physics run from March 9 to June 30, 2016 (Run 9, 79.6 live days) and Run 8, with a total of 3.3×10 4 kg-day exposure, the largest reported WIMP data set among dual-phase xenon detectors in the world to date.The PandaX-II detector has been described in detail in Ref. [5]. The liquid xenon target consists of a cylindrical TPC with dodecagonal cross section (opposite-side distance 646 mm), confined by the polytetrafluoroethylene (PTFE) reflective wall, and a vertical drift distance of 600 mm defined by the cathode mesh and gate grid located at the bottom and top. For each physical event, the prompt scintillation photons (S1) and the delayed electroluminescence photons (S2) from the ionized electrons are collected by two arrays of 55 Hamamatsu R11410-arXiv:1607.07400v3 [hep-ex] Hamamatsu R8520-406 1-inch PMTs serving as an active veto. The γ background, which produces electron recoil (ER) events, can be distinguished from the dark matter nuclear recoil (NR) using the S2-to-S1 ratio. During the data taking period in Run 9, a few diffe...

Noroviruses are genetically diverse RNA viruses associated with acute gastroenteritis in mammalian hosts. Phylogenetically, they can be segregated into different genogroups as well as P (polymerase)-groups and further into genotypes and P-types based on amino acid diversity of the complete VP1 gene and nucleotide diversity of the RNA-dependent RNA polymerase (RdRp) region of ORF1, respectively. In recent years, several new noroviruses have been reported that warrant an update of the existing classification scheme. Using previously described 2× standard deviation (sd) criteria to group sequences into separate clusters, we expanded the number of genogroups to 10 (GI-GX) and the number of genotypes to 49 (9 GI, 27 GII, 3 GIII, 2 GIV, 2 GV, 2 GVI and 1 genotype each for GVII, GVIII, GIX [formerly GII.15] and GX). Viruses for which currently only one sequence is available in public databases were classified into tentative new genogroups (GNA1 and GNA2) and genotypes (GII.NA1, GII.NA2 and GIV. NA1) with their definitive assignment awaiting additional related sequences. Based on nucleotide diversity in the RdRp region, noroviruses can be divided into 60 P-types (14 GI, 37 GII, 2 GIII, 1 GIV, 2 GV, 2 GVI, 1 GVII and 1 GX), 2 tentative P-groups and 14 tentative P-types. Future classification and nomenclature updates will be based on complete genome sequences and will be coordinated and disseminated by the international norovirus classification-working group.

SUMMARY Sapoviruses cause acute gastroenteritis in humans and animals. They belong to the genus Sapovirus within the family Caliciviridae . They infect and cause disease in humans of all ages, in both sporadic cases and outbreaks. The clinical symptoms of sapovirus gastroenteritis are indistinguishable from those caused by noroviruses, so laboratory diagnosis is essential to identify the pathogen. Sapoviruses are highly diverse genetically and antigenically. Currently, reverse transcription-PCR (RT-PCR) assays are widely used for sapovirus detection from clinical specimens due to their high sensitivity and broad reactivity as well as the lack of sensitive assays for antigen detection or cell culture systems for the detection of infectious viruses. Sapoviruses were first discovered in 1976 by electron microscopy in diarrheic samples of humans. To date, sapoviruses have also been detected from several animals: pigs, mink, dogs, sea lions, and bats. In this review, we focus on genomic and antigenic features, molecular typing/classification, detection methods, and clinical and epidemiological profiles of human sapoviruses.

Sequence analysis showed heterogeneity among 74 strains and distinct molecular characteristics of highly virulent strains and variants.

No abstract

Nitroreductase (NTR) can be overexpressed in hypoxic tumors, thus the selective and efficient detection of NTR is of great importance. To date, although a few optical methods have been reported for the detection of NTR in solution, an effective optical probe for NTR monitoring in vivo is still lacking. Therefore, it is necessary to develop a near-infrared (NIR) fluorescent detection probe for NTR. In this study, five NIR cyanine dyes with fluorescence reporting structure decorated with different nitro aromatic groups, Cy7-1-5, have been designed and explored for possible rapid detection of NTR. Our experimental results presented that only a para-nitro benzoate group modified cyanine probe (Cy7-1) could serve as a rapid NIR fluorescence-enhanced probe for monitoring and bioimaging of NTR. The structure-function relationship has been revealed by theoretical study. The linker connecting the detecting and fluorescence reporting groups and the nitro group position is a key factor for the formation of hydrogen bonds and spatial structure match, inducing the NTR catalytic ability enhancement. The in vitro response and mechanism of the enzyme-catalyzed reduction of Cy7-1 have been investigated through kinetic optical studies and other methods. The results have indicated that an electro-withdrawing group induced electron-transfer process becomes blocked when Cy7-1 is catalytically reduced to Cy7-NH2 by NTR, which is manifested in enhanced fluorescence intensity during the detection process. Confocal fluorescence imaging of hypoxic A549 cells has confirmed the NTR detection ability of Cy7-1 at the cellular level. Importantly, Cy7-1 can detect tumor hypoxia in a murine hypoxic tumor model, showing a rapid and significant enhancement of its NIR fluorescence characteristics suitable for fluorescence bioimaging. This method may potentially be used for tumor hypoxia diagnosis.

To understand the progression of porcine epidemic diarrhea virus infection, we inoculated gnotobiotic pigs with a newly emerged US strain, PC21A, of the virus. At 24–48 hours postinoculation, the pigs exhibited severe diarrhea and vomiting, fecal shedding, viremia, and severe atrophic enteritis. These findings confirm that strain PC21A is highly enteropathogenic.