Emerging and re-emerging coronaviruses in pigs

Wang, Qiuhong; Vlasova, Anastasia N.; Kenney, Scott P.; Saif, Linda J.

doi:10.1016/j.coviro.2018.12.001

Cited by 314 publications

(347 citation statements)

References 114 publications

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“…In this study, we explored interactions between SADS-CoV infection and the host innate immune for the first time. Previous studies revealed remarkable villus atrophy and severe pathological lesions in intestines of SADS-CoV inoculated piglets, indicating that the primary target cells of SASD-CoV in vivo are intestinal epithelial cells, which is same with other porcine enteric coronaviruses, PEDV and PDCoV (Zhou et al, 2018a;Xu et al, 2019;Koonpaew et al, 2019;Wang et al, 2019). Intestinal epithelium plays an important role in the intestinal mucosa immune response against pathogens (Temeeyasen et al, 2018).…”

Section: Discussionmentioning

confidence: 94%

“…SADS-CoV, PEDV and PDCoV are three emerging and highly pathogenic enteric coronaviruses in pigs in China (Wang et al, 2019). As a newly identified porcine enteric coronavirus, SADS-CoV seemed to share similarities on interactions with the host innate immune with PEDV and PDCoV (Table 2).…”

Section: Discussionmentioning

confidence: 99%

“…Following recognition, specific signal pathways of these receptors are activated to induce expressions of type I interferons. Then the type I interferon binds to its receptor to activate downstream JAK/STAT1 signaling pathway, which ultimately produces a series of interferon-stimulating factors that can inhibit the virus proliferation (Kawai and Akira, 2006;Carlos et al, 2007;Li et al, 2010). For most RNA viruses, double-stranded RNA (dsRNA) is the replicative intermediate, which is mainly recognized by retinoic acid-inducible gene I (RIG-I) and melanoma differentiationassociated gene 5 (MDA5) (Andrejeva et al, 2004;Cao et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

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Swine acute diarrhea syndrome coronavirus (SADS-CoV) antagonizes interferon-β production via blocking IPS-1 and RIG-I

et al. 2020

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A B S T R A C TSwine acute diarrhea syndrome coronavirus (SADS-CoV), a newly emerging enteric coronavirus, is considered to be associated with swine acute diarrhea syndrome (SADS) which has caused significantly economic losses to the porcine industry. Interactions between SADS-CoV and the host innate immune response is unclear yet. In this study, we used IPEC-J2 cells as a model to explore potential evasion strategies employed by SADS-CoV. Our results showed that SADS-CoV infection failed to induce IFN-β production, and inhibited poly (I:C) and Sendai virus (SeV)-triggered IFN-β expression. SADS-CoV also blocked poly (I:C)-induced phosphorylation and nuclear translocation of IRF-3 and NF-κB. Furthermore, SADS-CoV did not interfere with the activity of IFN-β promoter stimulated by IRF3, TBK1 and IKKε, but counteracted its activation induced by IPS-1 and RIG-I. Collectively, this study is the first investigation that shows interactions between SADS-CoV and the host innate immunity, which provides information of the molecular mechanisms underlying SASD-CoV infection.

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Section: Discussionmentioning

confidence: 94%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Swine acute diarrhea syndrome coronavirus (SADS-CoV) antagonizes interferon-β production via blocking IPS-1 and RIG-I

et al. 2020

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“…In April 2013, the Chinese-like PEDV variant also emerged in the United States and rapidly spread across the country (Stevenson et al, 2013;Vlasova et al, 2014). Currently, PEDV has been reported in many countries and causes high economic losses (Jung and Saif, 2015;Lee, 2015;Song et al, 2015;Wang et al, 2019;Zhang and Yoo, 2016).…”

Section: Introductionmentioning

confidence: 99%

Rapid manipulation of the porcine epidemic diarrhea virus genome by CRISPR/Cas9 technology

Peng

Fang

Ding

et al. 2020

Journal of Virological Methods

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“…Porcine deltacoronavirus (PDCoV), an emerging swine enteric coronavirus (CoV), belongs to the newly identified genus Deltacoronavirus, family Coronaviridae in the order Nidovirales (Ma et al, 2015;Wang et al, 2019). Clinically, PDCoV infection causes severe diarrhea and intestinal pathological damage in nursing piglets (Jung et al, 2016;Wang et al, 2016a;Zhang, 2016).…”

Section: Introductionmentioning

confidence: 99%

Porcine deltacoronavirus nsp15 antagonizes interferon-β production independently of its endoribonuclease activity

Liu

Fang

et al. 2019

Molecular Immunology

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Porcine deltacoronavirus (PDCoV) is an emerging swine coronavirus causing diarrhea and intestinal damage in nursing piglets. Previous work showed that PDCoV infection inhibits type I interferon (IFN) production. To further identify and characterize the PDCoV-encoded IFN antagonists will broaden our understanding of its pathogenesis. Nonstructural protein 15 (nsp15) encodes an endoribonuclease that is highly conserved among vertebrate nidoviruses (coronaviruses and arteriviruses) and plays a critical role in viral replication and transcription. Here, we found that PDCoV nsp15 significantly inhibits Sendai virus (SEV)-induced IFN-β production. PDCoV nsp15 disrupts the phosphorylation and nuclear translocation of NF-κB p65 subunit, but not antagonizes the activation of transcription factor IRF3. Interestingly, site-directed mutagenesis found that PDCoV nsp15 mutants (H129A, H234A, K269A) lacking endoribonuclease activity also suppress SEV-induced IFN-β production and NF-κB activation, suggesting that the endoribonuclease activity is not required for its ability to antagonize IFN-β production. Taken together, our results demonstrate that PDCoV nsp15 is an IFN antagonist and it inhibits interferon-β production via an endoribonuclease activity-independent mechanism.

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Emerging and re-emerging coronaviruses in pigs

Cited by 314 publications

References 114 publications

Swine acute diarrhea syndrome coronavirus (SADS-CoV) antagonizes interferon-β production via blocking IPS-1 and RIG-I

Swine acute diarrhea syndrome coronavirus (SADS-CoV) antagonizes interferon-β production via blocking IPS-1 and RIG-I

Rapid manipulation of the porcine epidemic diarrhea virus genome by CRISPR/Cas9 technology

Porcine deltacoronavirus nsp15 antagonizes interferon-β production independently of its endoribonuclease activity

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