Serological and molecular detection of selected hemoprotozoan parasites in donkeys in West Omdurman, Khartoum State, Sudan

Elata, Afraa; Mossaad, Ehab; Satti, Rawan A.; Matar, Nadia; Ohari, Yuma; Xuan, Xuenan; Inoue, Noboru; Suganuma, Keisuke

doi:10.1292/jvms.19-0534

Cited by 7 publications

(4 citation statements)

References 31 publications

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“…In other countries, seroprevalence of T. equi in horses have been various from 78.8% to 0.0% depending on its infection and sanitation status. Sudan has the highest seroprevalence at 78.8%, followed by Mongolia (72.8%), Brazil (34.8%), and China (3.8%) (Boldbaatar et al, 2005;Elata et al, 2020;Li et al, 2019;Souza et al, 2019;Xuan et al, 2002). In Japan, no T. equi antibody-positive cases were found (Huang et al, 2006).…”

Section: Resultsmentioning

confidence: 97%

“…The seroprevalence ofB. caballi was the highest in Mongolia at 40.1%, followed by Brazil (27.2%), China (20.1%), Japan (7%), and Sudan (5.1%) (Boldbaatar et al, 2005;Elata et al, 2020;Li et al, 2019;Souza et al, 2019;Xuan et al, 2002). To sustain "EP-free" status in ROK, a thorough border quarantine inspection should be conducted continuously and the monitoring of domesticated horses of EP should be performed consecutively.…”

Section: Resultsmentioning

confidence: 99%

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Molecular and serological surveillance of equine piroplasmosis in the Republic of Korea between 2016 and 2017

Seo¹,

Kim²,

Lee³

et al. 2020

Preprint

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Equine piroplasmosis (EP), a tick-borne disease affecting horses, is caused by Babesia caballi and Theileria equi. We investigate antigen and antibody of EP in whole bloods and sera of horses in the Republic of Korea during 2016-2017. We collected 1,650 whole bloods and sera of horses in 16 regions and 222 farms to examine antigen and antibody of EP by polymerase chain reaction (PCR) of 18S rRNA gene and enzyme-linked immunosorbent assay (ELISA), respectively. T. equi antigen and antibody was detected in 1 of 1,650 samples (0.06%). Phylogenetic analysis of 18S rRNA revealed that T. equi was highly homologous with the strains from China, Mongolia, or Spain. At EP-positive ranch, 52 and 10 ticks were collected in 2016 and 2017, respectively. All of them were identified as Haemaphysalis longicornis, but EP pathogens were not detected from the collected ticks. From 1,650 whole bloods, 2 of Theileria spp. were also detected and highly homologous with T. buffeli, T. luwehshuni, and T. orientalis group. EP infectious status was very low in Korea, and routine surveillance should be needed to attain the free status of EP in Korea.

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Section: Resultsmentioning

confidence: 97%

Section: Resultsmentioning

confidence: 99%

Molecular and serological surveillance of equine piroplasmosis in the Republic of Korea between 2016 and 2017

Seo¹,

Kim²,

Lee³

et al. 2020

Preprint

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show abstract

“…Even though B. caballi-positive horse was not found until now in Korea, the seroprevalence of B. caballi was also different from each country. The seroprevalence of B. caballi was the highest in Mongolia at 40.1%, followed by Brazil (27.2%), China (20.1%), Japan (7%), and Sudan (5.1%) [11][12][13][14]. To sustain "EP-free" status in ROK, thorough border quarantine inspection should be conducted continuously and the monitoring of EP for domesticated horses should be performed consecutively.…”

Section: Molecular and Serological Surveillance Of Equine Piroplasmosmentioning

confidence: 99%

“…Sudan has the highest seroprevalence at 78.8%, followed by Mongolia (72.8%), Brazil (34.8%), China (3.8%), and Japan (0.0%) [11][12][13][14][15]. However, no T. equi antibody-positive cases were found in Japan [12]. The seroprevalence (0.06%) of T. equi The 18S rRNA region sequences of T. equi and Theileria spp.…”

Section: Molecular and Serological Surveillance Of Equine Piroplasmosmentioning

confidence: 99%

Molecular and serological surveillance of equine piroplasmosis in the Republic of Korea between 2016 and 2017

Seo

Kim

Lee³

et al. 2021

Korean J Vet Res

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Short CommunicationEquine piroplasmosis (EP) is a tick-borne protozoal disease in which horses, mules, donkeys, and zebras are infected with the hemoprotozoan parasites, Babesia caballi or Theileria equi from blood-sucking ticks [1,2]. EP causes serious economic damages, showing hemolytic anemia and associated systemic illness [1]. Furthermore, EP has been an obstacle to international trade as the disease designated by World Organization for Animal Health [3][4][5]. EP is an endemic disease prevalent in the tropical and subtropical regions and in some temperate regions [1,2]. It has predominantly been reported in Asia, South and Central America, Africa, Southern Europe, and some parts of southern USA [1,2].EP antigen diagnosis is performed by microscopic examination and polymerase chain reaction (PCR), and the antibody diagnosis is performed by indirect fluorescent antibody test, competitive enzyme-linked immunosorbent assay (cELI-SA), and complement fixation test [1]. Because of the reliability of the test and the convenience of mass testing, antigen diagnosis by PCR and antibody diagnosis by cELISA are mainly used [2]. EP in the Republic of Korea (ROK) was 0.9% T. equi-positivity from three provinces during 2007-2010 [3,4]. Climate change is having a significant impact on the increase in ticks and tick-borne diseases [2]. There is a need to investigate changes in the infection status of EP according to Korean Peninsula warming. In this study, we monitored the EP infectious status in ROK during 2016-2017, using PCR for antigen detection and cELISA for antibody detection. Subsequently, phylogenetic analysis was used to compare the genetic relationships.Horse blood and serum samples were collected as a part of the health manage-Equine piroplasmosis (EP) is caused by Babesia caballi and Theileria equi infection. We investigated antigen and antibody of EP in horses in the Republic of Korea during 2016-2017. Antigen and antibody of T. equi was detected 0.06% (1/1,650). Phylogenetic analysis of 18S rRNA revealed that the T. equi was highly homologous with the strains from China, Mongolia, and Spain. Two Theileria spp. were also detected and highly homologous with T. buffeli, T. luwenshuni, and T. orientalis.

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First molecular detection and identification of Trypanosoma evansi in goats from Cebu, Philippines using a PCR-based assay

Elata

Galon

Moumouni

et al. 2020

Veterinary Parasitology: Regional Studies and Reports

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Serological and molecular detection of selected hemoprotozoan parasites in donkeys in West Omdurman, Khartoum State, Sudan

Cited by 7 publications

References 31 publications

Molecular and serological surveillance of equine piroplasmosis in the Republic of Korea between 2016 and 2017

Molecular and serological surveillance of equine piroplasmosis in the Republic of Korea between 2016 and 2017

Molecular and serological surveillance of equine piroplasmosis in the Republic of Korea between 2016 and 2017

First molecular detection and identification of Trypanosoma evansi in goats from Cebu, Philippines using a PCR-based assay

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