Sequential injection analysis system with spectrophotometric detection for determination of norfloxacin and ciprofloxacin in pharmaceutical formulations

Abstract

Recebido em 15/3/10; aceito em 17/9/10; publicado na web em 8/12/10 This work proposes a sequential injection analysis (SIA) system for the spectrophotometric determination of norfloxacin (NOR) and ciprofloxacin (CIP) in pharmaceutical formulations. The methodology was based on the reaction of these drugs with p-(dimethylamino)cinnamaldehyde in micellar medium, producing orange colored products (λ max = 495 nm). Beer´s law was obeyed in the concentration range from 2.75x10 -5 to 3.44x10 -4 mol L -1 and 3.26x10 -5 to 3.54x10 -4 mol L -1 for NOR and CIP, respectively and sampling rate was 25 h -1. Commercial samples were analyzed and results obtained through the proposed method were in good agreement with those obtained using the reference procedure for a 95% confidence level.

Convenient and sensitive detection of norfloxacin with fluorescent carbon dots

“…3 Therefore, NOR determination has become more and more important because of its increasingly widespread distribution in pharmaceuticals, food and the environment. To date, there are several methods reported in the literature for determining NOR or quinolones in pharmaceutical formulations and/or biologic uids, which include spectrophotometry, 4,5 high performance liquid chromatography, [6][7][8][9][10] charge transfer complex formation, 11,12 derivative UV spectrophotometry, 13,14 capillary electrophoresis, [15][16][17][18] uorescence, 19 SIA, 20,21 ow injection analysis [22][23][24][25] and immunoassay. 26 However, most of these methods present low sensitivity, 9 involving the use of organic solvents 6,7 or require expensive instruments.…”