Separation of iron-binding protein from whey through enzymatic hydrolysis

Properties of casein hydrolysate as affected by plastein reaction in ethanol-water medium

“…Bao et al (2007) found that the hydrolysate fractions with average molecular weight of 22.5 kDa had much higher calcium-binding ability than those with the weight of 8.76 kDa, and molecular weight, number of amino acid residues and functional groups contained in the peptide chain governed the calcium-binding ability of soybean protein hydrolysates. Whey protein hydrolysates digested by nine proteases in vitro had better iron-binding than whey protein concentrates, among which was Alcalase-generated hydrolysate possessing the highest iron-binding (Kim et al 2007b). This study showed that the differences in the type of amino acids, net charge and peptide length had an impact on the metal-binding of the hydrolysates, and gave a support to the present result, i.e.…”

Effect of Neutrase, Alcalase, and Papain Hydrolysis of Whey Protein Concentrates on Iron Uptake by Caco-2 Cells

“…It was summarized that these enhancers had 62 similar characteristics as: 1) contain some of "specific" amino acids, such as Pro, Phe, 63 accordant and the exact effect and mechanism were uncertain yet (10,11); 2) presence 65 of sulphydryl (-SH) groups. It was assumed that -SH could reduce Fe 3+ to more 66 soluble Fe 2+ (12); 3) have low molecular weight, it was reported that most of 67 enhancers had molecular weight (MW) lowered than 10 KDa (5,10,13,14). 68 Studies on protein showed that alcalase and flavourzyme have extensive proteolytic 69 activity for low molecular weight peptides and amino acids (13)(14)(15).…”

“…It was assumed that -SH could reduce Fe 3+ to more 66 soluble Fe 2+ (12); 3) have low molecular weight, it was reported that most of 67 enhancers had molecular weight (MW) lowered than 10 KDa (5,10,13,14). 68 Studies on protein showed that alcalase and flavourzyme have extensive proteolytic 69 activity for low molecular weight peptides and amino acids (13)(14)(15). However, effect 70 and application of these hydrolysates on fortification of minerals is scare.…”

Isolation of whey protein and hydrolysis pattern of whey protein by proteolytic enzyme

“…유청 단 백질 가수분해물들은 생리적으로 여러 가지 불만족스럽고 비정상적인 환자를 영양학적으로 지원하는데 광범위하게 이용되고 있다 (Halken and Host, 1997). 우유단백질을 효 소 가수분해한 연구들을 보면 유청단백질을 trypsin, papain 으로 가수분해하여 얻은 peptide 중에는 철 결합 능력이 있는 peptide를 얻었고 (Kim et al, 2007), β-LG을 pepsin 으로 가수분해하여 항원성의 변화와 IgE 결합 peptide에 관 한 보고가 있었다 (Chicon et al, 2008). 우수한 생리활성 기능을 가지는 몇몇 peptide는 효소에 의한 유청 단백질의 가수분해로 밝혀졌는데 예를 들면 opioid peptide (Meisel and FitzGerald, 2000), angiotensin I-converting 효소 저해 peptide (Gobbetti et al, 2004), antithrombotic peptide (Chabance et al, 1995), 면역조절 peptide (Mercier et al, 2004) 그리고 무기질 수송 peptide(Kim and Lim, …”