Properties of casein hydrolysate as affected by plastein reaction in ethanol-water medium

Abstract

Zhang Y., Zhao X.-H. (2013): Properties of casein hydrolysate as affected by plastein reaction in ethanol-water medium. Czech J. Food Sci., 31: 559-567.Casein hydrolysate with in vitro ACE-inhibitory activity of 44.4% at 0.3 mg/ml was generated from casein by Alcalase and modified by the Alcalase-catalysed plastein reaction in an ethanol-water medium. Eight treated hydrolysates were prepared using the reaction time of 1-8 h under ethanol or substrate concentration, Alcalase addition and reaction temperature of 56.8% (v/v) or 56.8% (w/v), 8.4 kU/g peptides and 37.5°C, respectively. Most of the treated hydrolysates showed enhanced ACE-inhibition compared to casein hydrolysate, and a reaction time of 4 h brought about the highest ACE-inhibition. All treated hydrolysates had lower zinc-or calcium-chelation but slightly higher iron(II)-chelation than casein hydrolysate, and a reaction time of 4 or 2 h could grant the treated hydrolysates the highest zinc-or calcium-chelation. Kinetic evaluation indicated that casein hydrolysate and two treated hydrolysates were competitive inhibitors to ACE. ACE-inhibition of these evaluated hydrolysates originated from themselves but was uncorrelated with their zinc-chelation, while their CaCO 3 precipitation inhibition was clearly correlated with their measured calcium-chelation (P < 0.05).

Use of Alcalase in the production of bioactive peptides: A review

“…Zhang and Zhao studied the hydrolysis of casein with Alcalase obtaining hydrolysates with in vitro angiotensin I-converting enzyme inhibitory activity of 44.4% [502]. The hydrolysates were later modified by Alcalase-catalyzed plastein reaction in an ethanol-water medium finding that most of the treated hydrolysates enhanced their angiotensin I-converting enzyme inhibition activities compared to the initial casein hydrolysate, mainly at 4 h of reaction time [502].…”

“…Zhang and Zhao studied the hydrolysis of casein with Alcalase obtaining hydrolysates with in vitro angiotensin I-converting enzyme inhibitory activity of 44.4% [502]. The hydrolysates were later modified by Alcalase-catalyzed plastein reaction in an ethanol-water medium finding that most of the treated hydrolysates enhanced their angiotensin I-converting enzyme inhibition activities compared to the initial casein hydrolysate, mainly at 4 h of reaction time [502]. The same authors reported the optimization of the Alcalase-catalyzed plastein reaction in ethanol-water medium to improve the in vitro angiotensin I-converting enzyme inhibitory activity (44.4%) of the Alcalase casein hydrolysate [503].…”

In VitroCalcium-Chelating and Platelet Anti-Aggregation Activities of Soy Protein Hydrolysate Modified by the Alcalase-Catalyzed Plastein Reaction

“…The plastein reaction generated six modified hydrolysates with different inhibitory effects on calcium precipitation. A previous study reported that there is a relationship between the calcium‐chelating activities of the modified casein hydrolysates and their inhibitory effects on calcium carbonate precipitation (Zhang and Zhao ). The results of that study were in accordance with the results obtained in this study.…”

“…The plastein reaction in water or other media enhanced the in vitro inhibition of angiotensin I converting enzyme, the antioxidant activity of protein hydrolysates (Zhao et al 2010;Sun and Zhao 2012;Yue et al 2013;Zhao and Zhao 2013) and the cytoprotection of casein hydrolysates on rat hepatocytes (Zhao et al 2014). The plastein reaction of casein hydrolysates in ethanol-water showed an impact on the mineral-chelating activity (including of calcium, zinc and iron) and inhibition of the calcium carbonate precipitation (Zhang and Zhao 2013). However, the effect of the plastein reaction on the mineral-chelating activity, calcium precipitation and platelet aggregation of SPH is unknown so far.…”

“…). The plastein reaction of casein hydrolysates in ethanol–water showed an impact on the mineral‐chelating activity (including of calcium, zinc and iron) and inhibition of the calcium carbonate precipitation (Zhang and Zhao ). However, the effect of the plastein reaction on the mineral‐chelating activity, calcium precipitation and platelet aggregation of SPH is unknown so far.…”