Effect of Neutrase, Alcalase, and Papain Hydrolysis of Whey Protein Concentrates on Iron Uptake by Caco-2 Cells

Ou, Keqin; Liu, Y.Z.; Zhang, Libin; Yang, Xianqing; Huang, Zhan; Nout, M.J.R.; Liang, Jiankun

doi:10.1021/jf100055y

Cited by 49 publications

(36 citation statements)

References 27 publications

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“…The reason for the more extensive proteolysis achieved with the Alcalase compared to Neutrase and papain could be its broader specificity toward the substrate and therefore its higher capacity for cleavage of various peptide bonds (Ou et al 2010;Kim et al 2007). Alcalase is a commercial preparation of relatively crude bacterial extract comprising several proteases with different specificities (Kim et al 2007).…”

Section: Resultsmentioning

confidence: 98%

“…Changes in both biological and functional properties of egg white are expected to occur during the hydrolysis by proteases, since the molecular weight (MW) of proteins decreases, hydrophobic groups become exposed, and reactive amino acid chains are disclosed (Ou et al 2010). Biological functions of obtained hydrolysates are dependent upon DH as well as the specificity of the applied protease.…”

Section: Resultsmentioning

confidence: 99%

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Production of Antioxidant Egg White Hydrolysates in a Continuous Stirred Tank Enzyme Reactor Coupled with Membrane Separation Unit

Jakovetić

Luković

Jugović

et al. 2014

Food Bioprocess Technol

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The objective of this research was to design an efficient continuously operated membrane reactor with a separation unit for egg white protein (EWP) hydrolysis and production of hydrolysates with improved antioxidant properties. For this purpose, a mechanically stirred tank reactor coupled with the polyethersulfone ultrafiltration module with a molecular weight cutoff of 10 kDa was employed. Several proteolytic enzymes have been tested in order to obtain the best quality of peptide-based formulations intended for human consumption. Among protease from Bacillus licheniformis (Alcalase), protease from Bacillus amyloliquefaciens (Neutrase), and protease from papaya latex (papain), the highest degree of hydrolysis (DH), as well as the best antioxidant properties of obtained hydrolysates, was achieved with Alcalase. The effects of operating variables such as enzyme/ substrate ([E]/[S]) ratio, impeller speed, and permeate flow rate were further studied using response surface methodology (RSM) and Box-Behnken experimental design. Results obtained in RSM analysis confirmed that over the studied range [E]/[S] ratio, impeller speed and permeate flow rate had the significant effect on the DH and reactor capacity. The effects of different impeller geometries were also studied and fourbladed propeller stirrer enabled the highest DH. Antioxidant properties were analyzed by the 2,2-diphenyl-1-picrylhydrazyl (DPPH), by the 2,2′-azino-bis-(3-ethylbenzothiazoline)-6-sulfonic acid (ABTS) radical scavenging activity, and by the linear voltammetry methods. Results show that the use of Alcalase in the membrane reactor system is of potential interest for the EWP hydrolysis and obtaining value-added egg products.

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Section: Resultsmentioning

confidence: 98%

Section: Resultsmentioning

confidence: 99%

Production of Antioxidant Egg White Hydrolysates in a Continuous Stirred Tank Enzyme Reactor Coupled with Membrane Separation Unit

Jakovetić

Luković

Jugović

et al. 2014

Food Bioprocess Technol

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“…Coupled with basement membranes, it provides a structural network for cell growth . Moreover, extracellular matrix sustains the repair process, binds cells together, provides cushioning and viscosity and serves as a medium for cell migration and diffusion of growth factors and other molecules . A wound is defined as an injury resulting from short term injury at a discrete site .…”

Section: Discussionmentioning

confidence: 99%

“…The gelatinases MMP‐2 and MMP‐9 complexes and their activity seem increased in dermal wounds in respect to intact and healthy skin . In fibroplasias and granulation tissue formation, fibroblasts grow and form a new, provisional extracellular matrix by excreting type I collagen . In the maturation and remodelling phase, collagen is remodelled and realigned along tension lines and cells that are no longer needed are removed by apoptosis.…”

Section: Discussionmentioning

confidence: 99%

“…Calcium ions and papain together carry out two synergetic functions, and those are the following: (i) Calcium ions in the form of salts cause a micro‐desiccation and a surface exfoliation of the wounds, eliminating the seriously damaged cells and correcting the peripheral dysemia (unbalance of mineral salts in the blood that flows into the dermal capillaries after a lesion) that occurs in the dermis after a trauma. (ii) Papain accelerates the absorption of the nutrients presented in the preparation …”

Section: Methodsmentioning

confidence: 99%

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A novel composition for in vitro and in vivo regeneration of skin and connective tissues

Gennero

Siena²,

Denysenko

et al. 2011

Cell Biochemistry & Function

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The particular combination of polydeoxyribonucleotides, l-carnitine, calcium ions, proteolytic enzyme and other ingredients acts in a synergetic way in the regeneration of skin and connective tissues. This new formulation of active principles was tested in vitro as a cell and tissue culture medium and in vivo for various preparations in support of tissue regeneration. In vitro, the new blend allowed the maintenance of skin biopsies for more than 1 year in eutrophic conditions. Immunocytochemical analyses of fibroblasts isolated from these biopsies confirmed a significant increase of the epidermal and connective wound-healing markers such as collagen type I, collagen type IV, cytokeratin 1 (CK1), CK5, CK10 and CK14 versus controls. To examine the effects of the new compound in vivo, we studied impaired wound healing in genetically diabetic db/db mice. At day 18, diabetic mice treated with the new composition showed 100% closure of wounds and faster healing than mice treated with the other solutions. This complex of vital continuity factors or life-keeping factors could be used as a tissue-preserving solution or a cosmetic/drug/medical device to accelerate wound healing in the treatment of patients with deficient wound repair to promote the regeneration of cutaneous and connective tissues (injuries-wound, dermatitis) and prevent the recurrent relapses.

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Proteolysis of Bovine Whey, Milk and Colostrum with Serine Endopeptidases

Halavach

2022

Lecture Notes in Networks and Systems

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Effect of Neutrase, Alcalase, and Papain Hydrolysis of Whey Protein Concentrates on Iron Uptake by Caco-2 Cells

Cited by 49 publications

References 27 publications

Production of Antioxidant Egg White Hydrolysates in a Continuous Stirred Tank Enzyme Reactor Coupled with Membrane Separation Unit

Production of Antioxidant Egg White Hydrolysates in a Continuous Stirred Tank Enzyme Reactor Coupled with Membrane Separation Unit

A novel composition for in vitro and in vivo regeneration of skin and connective tissues

Proteolysis of Bovine Whey, Milk and Colostrum with Serine Endopeptidases

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