Sensitive detection of potato spindle tuber viroid using RT-PCR and identification of a viroid variant naturally infecting pepino plants

Shamloul, A. M.; Hadidi, A.; Zhu, Shifeng; Singh, R. P.; Sagredo, Boris

doi:10.1080/07060669709500580

Cited by 76 publications

(53 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…At all four locations the NPPO confirmed the presence of PSTVd in all pepper selections by RT-PCR according to Shamloul et al (1997) and subsequent sequencing of the PCR products. The predominant sequence (GenBank Accession No.…”

Section: Figurementioning

confidence: 89%

“…Within the four locations, plant age varied from young plants that had just been grafted to plants starting fruit production. In none of the pepper plants were any symptoms observed.At all four locations the NPPO confirmed the presence of PSTVd in all pepper selections by RT-PCR according to Shamloul et al (1997) and subsequent sequencing of the PCR products. The predominant sequence (GenBank Accession No.…”

mentioning

confidence: 89%

See 1 more Smart Citation

First report of Potato spindle tuber viroid in vegetatively propagated plants of Capsicum annuum in The Netherlands

Verhoeven¹,

Voogd²,

Strik³

et al. 2016

New Disease Reports

View full text Add to dashboard Cite

Figure 1Over recent decades Potato spindle tuber viroid (PSTVd, genus Pospiviroid) has been found in increasingly various crops. It may cause severe diseases in potato and tomato crops but usually occurs as symptomless infections in ornamental species (Verhoeven et al., 2010). Pepper (Capsicum annuum) has been reported to show mild symptoms (Lebas et al., 2005). In 2016 a breeding company informed the National Plant Protection Organization (NPPO) of the Netherlands on the suspicion of PSTVd infections in four seedless pepper selections that had been propagated vegetatively. The suspected plants had been produced by a Dutch nursery that had imported the planting material from Israel. In the Netherlands, the plants were grown at four locations: two greenhouses of the same nursery at two locations (598 plants), a greenhouse for fruit production (1,616 plants) and another for demonstration purposes (100 plants) (EPPO, 2016). Within the four locations, plant age varied from young plants that had just been grafted to plants starting fruit production. In none of the pepper plants were any symptoms observed.At all four locations the NPPO confirmed the presence of PSTVd in all pepper selections by RT-PCR according to Shamloul et al. (1997) and subsequent sequencing of the PCR products. The predominant sequence (GenBank Accession No. KX370618), detected in fourteen out of fifteen sequences was identical to FM998548, a sequence of an isolate from S. jasminoides, and one sequence showed minor differences. Based on these results, it is assumed that PSTVd-infected plants of S. jasminoides were the source of infection in the pepper plants. Aligning the sequence FM998548 with the predominant sequences of isolates from Brugmansia spp. and S. jasminoides, B1 and S1, respectively (Verhoeven et al., 2010), showed deviations at seven nucleotide positions of which four are identical to B1 and three to S1 (Fig. 1). Since these deviations only occurred at positions where the sequences of S1 and B1 differ, the sequence FM998548 could have resulted from a single recombination event in the terminal right and variable domains (Keese & Symons, 1985) in a plant of S. jasminoides infected by both B1 and S1. The same deviations have also been found in an isolate from related pepper selections in Israel, indicating that infected planting material may have served as the pathway for introduction of PSTVd in peppers into The Netherlands.At the nursery site PSTVd was also found in five out of twelve other vegetatively propagated pepper selections. Testing of potentially infected deliveries from this nursery revealed only one additional finding at a fruit production location. All infected plants were destroyed under official control of the NPPO. In addition, the national authorities of five other EU member states were informed on the delivery of infected pepper selections to growers in their countries.The findings of PSTVd in vegetatively propagated selections of C. annuum show that new propagation strategies of crops may result in additional risks ...

show abstract

Section: Figurementioning

confidence: 89%

mentioning

confidence: 89%

First report of Potato spindle tuber viroid in vegetatively propagated plants of Capsicum annuum in The Netherlands

Verhoeven¹,

Voogd²,

Strik³

et al. 2016

New Disease Reports

View full text Add to dashboard Cite

show abstract

“…Le test peut Ctre rialist par tlectrophortse sur gel de polyacrylamide (RT-PAGE) (OEPPEPPO, 1984; modifit par Huttinga eta/., 1987; Schroeder & Weidemann, 1989). par des sondes d'ARN (EU, 1997a) ou par la PCR (Shamloul et a/.. 1997;Weidemann & Buchta, 1998).…”

Section: Test De Detection Pour Le Potato Spindle Tuber Viroid (Pstvd)unclassified

Seed potatoes

1999

EPPO Bulletin

View full text Add to dashboard Cite

Approbation et amendement specifiques Specific definitions Seed potatoesTubers and microplants of cultivated tuber-forming Solanurn spp., which are produced under an official certification system to meet specified requirements. Definitions specifiques Pommes de terre de semence Microplants of potatoPlants (including tubers) in tissue culture of tuber-forming Solanurn SPP. Minitubers of potatoTubers produced by microplants of potato in a growing medium meeting specified requirements. Outline of the schemeThe scheme has the aim of providing seed potatoes that are free from certain pests and meet specified tolerances for others, and whose health status is attested by an official certificate. It does not cover farm-saved tubers or potato germplasm (tubers or microplants to be used as breeding material or true potato seeds). For the production of certified seed potatoes, the following successive steps should be followed by an official organization or under its control:1 Selection for quality of individual candidate nuclear stock plants of each cultivar to be taken into account in the scheme. Optional selection for virus freedom among these plants by testing. 2 Micropropagation of these plants. Selection for freedom from viruses and bacteria among microplants by testing or production of virus-free plants by treatment or in vitro methods, followed by testing. The microplants thus shown to be free from the given viruses and bacteria are designated as nuclear stock. 3 Maintenance of nuclear stock as microplants. 4 Multiplication of nuclear stock in two phases, propagation stock I and 11, respectively, under protected conditions and in the field, respectively, with retesting as appropriate, under rigorous conditions excluding reinfestation by certain pests and reducing reinfestation by others.

show abstract

“…These findings were further confirmed by sequence analysis of RT-PCR amplicons produced utilising primers Pospi1-FW/RE from a new extraction of a duplicate sample. Further RT-PCR analyses were performed using primers PSTVd-F/R (Shamloul et al, 1997) and CEVd-specific-S/AS (Semancik et al, 1993) to generate full-length genome sequences, which were submitted to GenBank with Accession Nos. KC282384 (PSTVd), KC282382 (CEVd, Petunia), KC282383 (CEVd, Petunia), and KC282381(CEVd, petchoa).…”

mentioning

confidence: 99%

First report of pospiviroids infecting ornamental plants in Australia: Potato spindle tuber viroid in Solanum laxum (synonym S. jasminoides) and Citrus exocortis viroid in Petunia spp.

Brunschot

Persley²,

Roberts³

et al. 2014

New Disease Reports

View full text Add to dashboard Cite

Sensitive detection of potato spindle tuber viroid using RT-PCR and identification of a viroid variant naturally infecting pepino plants

Cited by 76 publications

References 28 publications

First report of Potato spindle tuber viroid in vegetatively propagated plants of Capsicum annuum in The Netherlands

First report of Potato spindle tuber viroid in vegetatively propagated plants of Capsicum annuum in The Netherlands

Seed potatoes

First report of pospiviroids infecting ornamental plants in Australia: Potato spindle tuber viroid in Solanum laxum (synonym S. jasminoides) and Citrus exocortis viroid in Petunia spp.

Contact Info

Product

Resources

About