First report of pospiviroids infecting ornamental plants in Australia: Potato spindle tuber viroid in Solanum laxum (synonym S. jasminoides) and Citrus exocortis viroid in Petunia spp.

Brunschot, Sharon van; Persley, D. M.; Roberts, A. V.; Thomas, J. E.

doi:10.5197/j.2044-0588.2014.029.003

Cited by 13 publications

(4 citation statements)

References 5 publications

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“…CBCVd) was considered as minor pathogen in the genus Citrus 11 , but it was associated with severe bark cracking on trifoliate orange rootstock 15 – 17 . CEVd was known to have a wide host range 6 , 18 , 19 , and symptoms in different species of citrus, such as stunting, bark sloughing and cracking, leaf epinasty and cracks in the petiole 20 – 25 . CVd-III was reported to have of particular interest due to its genomic variability beside its significant reduction of growth and yield 26 – 29 .…”

Section: Introductionmentioning

confidence: 99%

Molecular detection of Citrus exocortis viroid (CEVd), Citrus viroid-III (CVd-III), and Citrus viroid-IV (CVd-IV) in Palestine

Abualrob,

Alabdallah,

Kubaa

et al. 2024

Sci Rep

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Citrus hosts various phytopathogens that have impacted productivity, including viroids. Missing data on the status of viroids in citrus in Palestine were not reported. This study was aimed to detect any of Citrus exocortis viroid (CEVd), Citrus viroid-III (CVd-III), and Citrus viroid-IV (CVd-IV) in the Palestinian National Agricultural Research Center (NARC) germplasm collection Field inspections found symptoms such as leaf epinasty; vein discoloration, and bark cracking on various citrus varieties. RT-PCR revealed a significant prevalence of CVd-IV; CEVd and CVd-III (47%, 31%, and 22%; respectively). CVd-III variants with 91.3% nucleic acid sequence homology have been reported. The sequence of each viroid were deposited in GenBank as (OP925746 for CEVd, OP902248 and OP902249 for CVd-III-PS-1 and -PS-2 isolates, and OP902247 for CVd-IV). This was the first to report three of citrus viroids in Palestine, appealing to apply of phytosanitary measures to disseminate healthy propagating materials free from viroids.

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Section: Introductionmentioning

confidence: 99%

Molecular detection of Citrus exocortis viroid (CEVd), Citrus viroid-III (CVd-III), and Citrus viroid-IV (CVd-IV) in Palestine

Abualrob,

Alabdallah,

Kubaa

et al. 2024

Sci Rep

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“…

has a global distribution and a broad host range including woody plants such as citrus, grape and fig (Semancik et al 1973;García-Arenal et al 1987;Yakoubi et al 2007), as well as herbaceous plants such as tomato, eggplant, carrot, broad bean and petunia (Mishra et al 1991;Fagoaga et al 1995;Fagoaga and Durán-Vila 1996;van Brunschot et al 2014). The viroid is mechanically transmissible (Barbosa et al 2005) and, importantly for perennial crops such as citrus, is disseminated in asymptomatically-infected, vegetative propagules such as budwood.

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mentioning

confidence: 99%

A reverse transcription loop-mediated isothermal amplification assay for the detection of citrus exocortis viroid in Australian citrus

Chambers

Geering

Holford

et al. 2023

Australasian Plant Pathol.

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Citrus exocortis viroid (CEVd), the causal agent of exocortis, is a pathogen that is thought to infect all citrus varieties, although it is asymptomatic in most. Symptoms of exocortis develop on susceptible rootstocks, resulting in stunting and yield reduction. To aid the detection and management of CEVd, a rapid near-field assay was developed using reverse transcription loop-mediated isothermal amplification (RT-LAMP) for the detection of the viroids in nursery and field trees. Over 240 CEVd sequences, including sequence variants from representative Australian isolates that induce mild and severe symptoms, were used in the design of the primers. The RT-LAMP successfully detected CEVd in a 1:1000 dilution (236 pg) of plant total RNA indicating high sensitivity, and also detected the viroid in rapid, crude plant extractions. The assay was highly specific to CEVd, given there was no cross-reactivity with other citrus-infecting pathogens. This new assay provides a simple, robust, specific, and sensitive method to detect CEVd in Australian citrus and to our knowledge, is the first RT-LAMP assay to detect any citrus-infecting viroid.

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“…(Van Brunschot et al, 2014). La transmisión del viroide se lleva a cabo por injerto de yemas infectadas en árboles sanos o de forma of both indexing methods was 99 % with initial symptoms tenth days after the in vitro inoculation and 40 in vivo.…”

unclassified

“…(Van Brunschot et al, 2014). The transmission of the viroid was carried out by grafting infected buds in healthy trees, or mechanically (Lin et al, 2015a) with the use of a cutting instrument and contaminated trimming.…”

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confidence: 99%

Caracterización del Viroide exocortis de los cítricos en diferentes condiciones de indexado

AlcÃ¡ntara-Mendoza¹,

Vergara-Pineda²,

GarcÃa-Rubio³

et al. 2017

RMF

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Resumen. Citrus exocortis viroid (CEVd) es unfitopatógeno regulado en la citricultura de México. El diagnóstico por indexado para detectar la presencia y transmisión de CEVd en material propagativo de bancos de germoplasma es de aplicación oficial. El objetivo de este estudio fue caracterizar al CEVd en condiciones de indexado in vitro e in vivo comparando la eficiencia de transmisión y la rapidez con que se generan los síntomas para establecer su aplicación en el diagnóstico biológico. Characterization of Abstract. Citrus exocortis viroid (CEVd) is aregulated pathogen in Mexican citriculture. The diagnosis by indexing to detect the presence and transmission of CEVd in propagative material of germplasm banks is of official application. The aim of this study was characterizing to CEVd by in vitro and in vivo indexing to analyzing the efficiency of the transmission and the speed with which the symptoms are generated to establish their application in the biological diagnosis. The height, incidence, area under the disease progress curve (ABCP) and transmission efficiency were evaluated in etrog citron indicator plants, cultivated in vivo inoculated by grafting and in buds cultivated in vitro inoculated with callus. The mean height of buds and infected plants was 7.5 mm and 56.3 mm less than the control, incidence, ABC and regression model were 81.2%, 10.7, y= 0.0009x 2 + 0.0693x-0.0057, R 2 = 0.99 in vitro y 70.8%, 9.84, y= 0.0004x 2 -0.0048x-0.0061, R 2 = 0.94 in vivo, respectively. The efficiency

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First report of pospiviroids infecting ornamental plants in Australia: Potato spindle tuber viroid in Solanum laxum (synonym S. jasminoides) and Citrus exocortis viroid in Petunia spp.

Cited by 13 publications

References 5 publications

Molecular detection of Citrus exocortis viroid (CEVd), Citrus viroid-III (CVd-III), and Citrus viroid-IV (CVd-IV) in Palestine

Molecular detection of Citrus exocortis viroid (CEVd), Citrus viroid-III (CVd-III), and Citrus viroid-IV (CVd-IV) in Palestine

A reverse transcription loop-mediated isothermal amplification assay for the detection of citrus exocortis viroid in Australian citrus

Caracterización del Viroide exocortis de los cítricos en diferentes condiciones de indexado

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