Diaporthe vaccinii causes twig blight and fruit rot and is currently listed as a quarantine organism for the European Union. In the Netherlands, two species from the same genus, Diaporthe eres and Diaporthe rudis, are regularly isolated from blighted twigs of Vaccinium corymbosum. This study compared the pathogenicity of these two species to D. vaccinii. To develop a pathogenicity test a field experiment was performed at an experimental station, testing isolates of D. rudis and D. eres. Most of the isolates tested did not cause statistically significantly larger lesions compared to the control treatment (P > 0.05). In a greenhouse experiment a similar test was conducted, comparing D. vaccinii with the closely related species D. eres. Two cultivars of blueberry, Duke and Liberty, were inoculated using two D. vaccinii and two D. eres isolates. Stem canker lesions caused by D. vaccinii were larger than these caused by D. eres isolates. The majority of the lesions caused by D. vaccinii did not pass the first node of the inoculated shoot, which might be an explanation for the ‘mild’ symptoms of D. vaccinii in V. corymbosum, which appear not to cause crop losses. The results of this study suggest that D. vaccinii may not be a major threat to blueberry production in Europe. Further data is required to be able to conclude whether the quarantine status of D. vaccinii is still appropriate.

Three different versions of the conventional PCR described by Bulman & Marshall () for the identification of cysts and juveniles of Globodera pallida and G. rostochiensis were compared: the original Bulman & Marshall, Bulman & Marshall as described in EPPO PM 7/40 (2) and an in‐house modified version of the Dutch National Plant Protection Organization (NPPO‐NL). The versions differ from each other in thermocycler conditions and primer sequences. Two different polymerases (Invitrogen and Roche) were assessed using the different test versions, and performance criteria analytical sensitivity and analytical specificity were determined. Roche‐based reaction mixes had the highest amplicon yield and were used for further comparison of the different test versions. The different test versions performed equally well in terms of analytical specificity. No false positive or false negative results were observed. The test version NPPO‐NL proved to be the most sensitive test version with a limit of detection of 1 juvenile for both G. pallida and G. rostochiensis.

Figure 1Over recent decades Potato spindle tuber viroid (PSTVd, genus Pospiviroid) has been found in increasingly various crops. It may cause severe diseases in potato and tomato crops but usually occurs as symptomless infections in ornamental species (Verhoeven et al., 2010). Pepper (Capsicum annuum) has been reported to show mild symptoms (Lebas et al., 2005). In 2016 a breeding company informed the National Plant Protection Organization (NPPO) of the Netherlands on the suspicion of PSTVd infections in four seedless pepper selections that had been propagated vegetatively. The suspected plants had been produced by a Dutch nursery that had imported the planting material from Israel. In the Netherlands, the plants were grown at four locations: two greenhouses of the same nursery at two locations (598 plants), a greenhouse for fruit production (1,616 plants) and another for demonstration purposes (100 plants) (EPPO, 2016). Within the four locations, plant age varied from young plants that had just been grafted to plants starting fruit production. In none of the pepper plants were any symptoms observed.At all four locations the NPPO confirmed the presence of PSTVd in all pepper selections by RT-PCR according to Shamloul et al. (1997) and subsequent sequencing of the PCR products. The predominant sequence (GenBank Accession No. KX370618), detected in fourteen out of fifteen sequences was identical to FM998548, a sequence of an isolate from S. jasminoides, and one sequence showed minor differences. Based on these results, it is assumed that PSTVd-infected plants of S. jasminoides were the source of infection in the pepper plants. Aligning the sequence FM998548 with the predominant sequences of isolates from Brugmansia spp. and S. jasminoides, B1 and S1, respectively (Verhoeven et al., 2010), showed deviations at seven nucleotide positions of which four are identical to B1 and three to S1 (Fig. 1). Since these deviations only occurred at positions where the sequences of S1 and B1 differ, the sequence FM998548 could have resulted from a single recombination event in the terminal right and variable domains (Keese & Symons, 1985) in a plant of S. jasminoides infected by both B1 and S1. The same deviations have also been found in an isolate from related pepper selections in Israel, indicating that infected planting material may have served as the pathway for introduction of PSTVd in peppers into The Netherlands.At the nursery site PSTVd was also found in five out of twelve other vegetatively propagated pepper selections. Testing of potentially infected deliveries from this nursery revealed only one additional finding at a fruit production location. All infected plants were destroyed under official control of the NPPO. In addition, the national authorities of five other EU member states were informed on the delivery of infected pepper selections to growers in their countries.The findings of PSTVd in vegetatively propagated selections of C. annuum show that new propagation strategies of crops may result in additional risks ...

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