Sensitive and robust MALDI-TOF-MS glycomics analysis enabled by Girard's reagent T on-target derivatization (GTOD) of reducing glycans

Zhang, Ying; Wang, Bo; Jin, Wanjun; Wen, Yanan; Nan, Lijing; Yang, Mingming; Liu, Rendan; Zhu, Yu; Wang, Chengjian; Huang, Linjuan; Song, Xuezheng; Wang, Zhongfu

doi:10.1016/j.aca.2018.10.015

Cited by 52 publications

(41 citation statements)

References 62 publications

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“…The presence of trimethylamine in the GT compound can increase the positive charge on the cryptobrachytone bonds with the GT, increasing the sensitivity to detection by MS. So, cryptobrachytone C would ionize to the maximum (Zhang et al 2019). Derivatization and MS/MS technique showed that cryptobrachytone C could be extracted by α-CHCA optimally in m/z 343.17.…”

Section: Discussionmentioning

confidence: 99%

Profile of cryptobrachytone C accumulation in Cryptocarya pulchrinervia leaves using MALDI-MSI

et al. 2021

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Abstract. Ratnasari J, Esyanti RR, Tan MI, Juliawaty LD, Shimma S. 2021. Profile of cryptobrachytone C accumulation in Cryptocarya pulchrinervia leaves using MALDI-MSI. Biodiversitas 22: 1172-1178. Cryptobrachytone C is an active compound isolated from leaves of Cryptocarya pulchrinervia, an indigenous plant from Indonesia. Cryptobrachytone C is cytotoxic against P388 leukemia cancer cell lines. A profile of cryptobrachytone C accumulation in leaves based on physiological age is necessary to study cryptobrachytone C production in the plant since it has anticancer potential. In situ profiling of cryptobrachytone C accumulation in leaves was carried out by imaging techniques using matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI). The cryptobrachytone C ionization efficiency was carried out by derivatization using Girard-T reagent and coating with α-cyano-4-hydroxycinnamic acid (α-CHCA) matrix. Scanned leaves show higher accumulation intensity in the second leaf. Quantitative accumulation profiling was conducted using GC-MS, where the highest amount of cryptobrachytone C was on the second leaf at 87.07 ± 47.21 mg. This showed that the most effective isolation of cryptobrachytone C would be obtained from young leaves of Cryptocarya pulchrinervia. This profile would play a role in cryptobrachytone C production in-situ or ex-situ using tissue culture.

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Section: Discussionmentioning

confidence: 99%

Profile of cryptobrachytone C accumulation in Cryptocarya pulchrinervia leaves using MALDI-MSI

et al. 2021

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“…In this approach, typically neutral HMOs and N-/O-glycans can be quantified using MALDI-MS because of unstable sialic acid residues found in HMOs and glycoconjugates. However, sialic acids can be derivatized by certain methods to make them more stable during the MALDI-MS analysis (134).…”

Section: Challenges In the Study And Characterization Of N-glycansmentioning

confidence: 99%

Potential Applications of Endo-β-N-Acetylglucosaminidases From Bifidobacterium longum Subspecies infantis in Designing Value-Added, Next-Generation Infant Formulas

et al. 2021

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Human milk is the optimal source of infant nutrition. Among many other health benefits, human milk can stimulate the development of a Bifidobacterium-rich microbiome through human milk oligosaccharides (HMOs). In recent years, the development of novel formulas has placed particular focus on incorporating some of the beneficial functional properties of human milk. These include adding specific glycans aimed to selectively stimulate the growth of Bifidobacterium. However, the bifidogenicity of human milk remains unparalleled. Dietary N-glycans are carbohydrate structures conjugated to a wide variety of glycoproteins. These glycans have a remarkable structural similarity to HMOs and, when released, show a strong bifidogenic effect. This review discusses the biocatalytic potential of the endo-β-N-acetylglucosaminidase enzyme (EndoBI-1) from Bifidobacterium longum subspecies infantis (B. infantis), in releasing N-glycans inherently present in infant formula as means to increase the bifidogenicity of infant formula. Finally, the potential implications for protein deglycosylation with EndoBI-1 in the development of value added, next-generation formulas are discussed from a technical perspective.

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“…As a result, increased levels of tri‐antennary, tetra‐antennary, and galactosylated glycan structures were observed. Compared with the in‐tube derivatization approach where sample loss easily occurs because of the labor‐intensive sample preparation and incomplete derivatization, the on‐target derivatization approach effectively overcame these aspects using specific matrix 3‐aminoquinoline or Girard's reagent T …”

Section: Ms Analysis Of Clinical Biomoleculesmentioning

confidence: 99%

Mass Spectrometry Methods for In Situ Analysis of Clinical Biomolecules

Liu

et al. 2019

Small Methods

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past few decades, great efforts and substantial progress have been made in this field, and various methods, such as colorimetric screening, [3] enzyme-linked immunosorbent assay (ELISA), [4] fluorescence, [5] polymerase chain reaction (PCR), [6] and electrochemical assays, [7] have been extensively performed in clinical laboratory tests. Although with efficiency and robustness, the majority of these methods still lack sufficient accuracy, sensitivity, and specificity for clinical diagnostic applications.The inevitable complexity and challenges of clinical biomolecules detection have encouraged researchers to continuously devote great effort to the development of new methods with accuracy, rapidity, and simplicity. Relying on its high throughput and accuracy, great sensitivity and versatility, reliable qualitative and quantitative ability, and multiplexed detection capability, mass spectrometry (MS) is exceptionally suitable for clinical analysis and blooming in modern healthcare industry in recent years. [8][9][10] MS coupled to liquid chromatography (LC) and gas chromatography (GC) is regarded as gold standards for quantitative analysis of various compounds and have been conventionally used in clinical applications such as toxicology, [11] therapeutic drug monitoring, [12] inborn error of metabolism (IEM), [13] and steroid hormone testing [14] for decades. IEMs cause high morbidity and mortality in children, and the utilization of GC-MS and LC-MS expands newborn IEMs screening from single-screening assays to simultaneously screening of various analytes (e.g., amino acids, organic acids, carnitine, and acylcarnitine) in a single run using one sample from the patient. Some reviews have summarized a number of excellent works about applications of GC-MS and LC-MS in clinical chemistry. [15,16] These hyphenated MS methods provide accurate quantification data and abundant information of clinical biomolecules, which facilitates biological behavior understanding, biomarker discovery, and prognosis evaluation. However, in most cases, these methods are conducted by using lysis of cells or tissues, which fail to provide real-time information and spatial distribution of biomolecules in their native states. On the other hand, tedious sample pretreatment (separation, purification, and redissolution) is inevitable. These series of labor intensive and time-consuming procedures may cause unpredictable sample loss and undervaluation of biomolecules. Therefore, chromatography-based MS methods are limited to achieve high-throughput, in situ, and visual clinical applications. The development of emerging Due to the significant role of clinical biomolecules in mediating biological activities and disease progressions, in situ analysis provides a great opportunity for understanding the molecular mechanisms of biological functions, facilitating medical diagnosis, clinical treatment, and prognosis. Among all the analysis methods, mass spectrometry (MS) methodologies exhibit unique features that make them exceptionally suitable for clinica...

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Sensitive and robust MALDI-TOF-MS glycomics analysis enabled by Girard's reagent T on-target derivatization (GTOD) of reducing glycans

Cited by 52 publications

References 62 publications

Profile of cryptobrachytone C accumulation in Cryptocarya pulchrinervia leaves using MALDI-MSI

Profile of cryptobrachytone C accumulation in Cryptocarya pulchrinervia leaves using MALDI-MSI

Potential Applications of Endo-β-N-Acetylglucosaminidases From Bifidobacterium longum Subspecies infantis in Designing Value-Added, Next-Generation Infant Formulas

Mass Spectrometry Methods for In Situ Analysis of Clinical Biomolecules

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