Selective removal of the autoantibodies from rheumatoid arthritis patient plasma using protein A carrying affinity cryogels

Heparin removal from human plasma using molecular imprinted cryogels

“…The measurement of backpressure of a chromatographic column at different flow rates is also crucial parameter for the characterization of physical property of column (Alkan et al 2009(Alkan et al , 2010. It effects retention times of compounds especially in the case of working with viscous samples.…”

Poly(hydroxyethyl methacrylate) based affinity cryogel for plasmid DNA purification

“…Supermacroporous cryogels are a very good alternative for the purification of large molecules like DNA with many advantages including large pores and short diffusion path [20][21][22][23][24][25][26]. The large pores of cryogels will allow penetration of large pDNA molecules to the internal surface area at high flow rate with low pressure drops [27].…”

Molecular imprinting based composite cryogel membranes for purification of anti-hepatitis B surface antibody by fast protein liquid chromatography

“…5). Anti-HBs adsorption capacity of the MI-CMs and the NI-CMs decreased from 338.0 mIU/g to 4.81 mIU/g for MI-CMs and from 213.0 mIU/g to 3.11 mIU/g for NI-CMs when the NaCl concentration increased to 0.5 M. According to the results, ionic interactions gave an essential contribution to the recognition and binding process [36]. A possible explanation for this phenomenon could be in two ways; (i) Counter salt ions interact with the antiHBs molecules via charge-charge interactions and mask the binding sites, and (ii) decrease in the adsorption capacity due to the increase in ionic strength can be attributed to the repulsive electrostatic forces between the MI-CMs and anti-HBs molecules [37].…”