Molecular imprinting based composite cryogel membranes for purification of anti-hepatitis B surface antibody by fast protein liquid chromatography

Aslıyüce, Sevgi; Uzun, Lokman; Rad, Abbas Yousefi; Ünal, Serhat; Say, Rıdvan; Deni̇zli̇, Adil

doi:10.1016/j.jchromb.2012.02.001

Cited by 76 publications

(54 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Cryogels are very good alternatives for protein purification with their promising advantages and there is an increasing interest to use them as adsorbents for diverse applications including protein purification and biomedical therapy [36][37][38][39]. In this study, we have focused our attention on developing a dye affinity cryogel for affinity adsorption of interferon molecules from both artificial and natural source using cryogels.…”

Section: Resultsmentioning

confidence: 99%

Evaluation of human interferon adsorption performance of Cibacron Blue F3GA attached cryogels and interferon purification by using FPLC system

Doğan

Özkara

Sarı

et al. 2012

Journal of Chromatography B

Self Cite

View full text Add to dashboard Cite

Section: Resultsmentioning

confidence: 99%

Evaluation of human interferon adsorption performance of Cibacron Blue F3GA attached cryogels and interferon purification by using FPLC system

Doğan

Özkara

Sarı

et al. 2012

Journal of Chromatography B

Self Cite

View full text Add to dashboard Cite

“…The scanning of the numerous polymers authorized a definite correlation between the binding features of the NIP and MIP libraries [17]. MIPs have been synthesized successfully using different types of molecular imprinting methods including surface imprinting, micro-contact imprinting and epitope imprinting and these polymers have been used in many applications such as purification [18], isolation [19], chiral separation [20], catalysis [21] and in sensors [22]. …”

Section: Molecular Imprinting Methodsmentioning

confidence: 99%

Molecular Imprinting of Macromolecules for Sensor Applications

Saylan

Yılmaz

Özgür

et al. 2017

Sensors

Self Cite

139

View full text Add to dashboard Cite

Molecular recognition has an important role in numerous living systems. One of the most important molecular recognition methods is molecular imprinting, which allows host compounds to recognize and detect several molecules rapidly, sensitively and selectively. Compared to natural systems, molecular imprinting methods have some important features such as low cost, robustness, high recognition ability and long term durability which allows molecularly imprinted polymers to be used in various biotechnological applications, such as chromatography, drug delivery, nanotechnology, and sensor technology. Sensors are important tools because of their ability to figure out a potentially large number of analytical difficulties in various areas with different macromolecular targets. Proteins, enzymes, nucleic acids, antibodies, viruses and cells are defined as macromolecules that have wide range of functions are very important. Thus, macromolecules detection has gained great attention in concerning the improvement in most of the studies. The applications of macromolecule imprinted sensors will have a spacious exploration according to the low cost, high specificity and stability. In this review, macromolecules for molecularly imprinted sensor applications are structured according to the definition of molecular imprinting methods, developments in macromolecular imprinting methods, macromolecular imprinted sensors, and conclusions and future perspectives. This chapter follows the latter strategies and focuses on the applications of macromolecular imprinted sensors. This allows discussion on how sensor strategy is brought to solve the macromolecules imprinting.

show abstract

“…Molecularly imprinted polymers (MIPs) which are currently known as plastic antibodies are synthetic polymers which can be designed and synthesised to recognize and specifically bind to a target molecule [55,77]. They are more resistant to chemical and biological damage and inactivation than antibodies [78]. A characteristic feature of MIPs is their hydrophobicity which allows for the weak reversible interactions between the target and the MIPs.…”

Section: Affinity Ligandsmentioning

confidence: 99%