Poly(hydroxyethyl methacrylate) based affinity cryogel for plasmid DNA purification

Perçin, Işık; Saglar, Emel; Yavuz, Handan; Aksöz, Erol; Deni̇zli̇, Adil

doi:10.1016/j.ijbiomac.2011.01.022

Cited by 60 publications

(29 citation statements)

References 47 publications

(39 reference statements)

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“…One of the proposed methods to solve these problems is to carry out the polymerization under mild condition such as low temperature [4]. Cryogels are the prominent alternatives; because, not only offering solution to the mentioned problems but also serving unique properties such as large inter-connected flow channels, low back pressure, low diffusion resistance, rapid and efficient adsorption dynamics for separation and purification of interested molecules [38][39][40]. In this study, we aimed to prepare molecular imprinted cryogel membranes for chromatographic purification of anti-HBs molecules from human plasma.…”

Section: Resultsmentioning

confidence: 99%

Molecular imprinting based composite cryogel membranes for purification of anti-hepatitis B surface antibody by fast protein liquid chromatography

Aslıyüce

Uzun

Rad

et al. 2012

Journal of Chromatography B

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Section: Resultsmentioning

confidence: 99%

Molecular imprinting based composite cryogel membranes for purification of anti-hepatitis B surface antibody by fast protein liquid chromatography

Aslıyüce

Uzun

Rad

et al. 2012

Journal of Chromatography B

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“…Also, a 16‐mer peptide that had been immobilized on a monolithic column permitted the purification of supercoiled plasmid DNA in a single step 115, 116. Pseudoaffinity chromatography for the purification of plasmid DNA was performed with a monolithic cryogel with copolymerized N ‐methacryloyl‐( L )‐histidine methylester 117.…”

Section: Types Of Acmentioning

confidence: 99%

Monolithic media for applications in affinity chromatography

Sproß

Sinz

2011

J of Separation Science

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Affinity chromatography presents a highly versatile analytical tool, which relies on exploiting highly specific interactions between molecules and their ligands. This review covers the most recent literature on the application of monoliths as stationary phases for various affinity-based chromatographic applications. Different affinity approaches as well as separations using molecularly imprinted monoliths are discussed. Hybrid stationary phases created by embedding of particles or nanoparticles into a monolithic stationary phase are also considered in this review article. The ease of preparation of monoliths and the multitude of functionalization techniques, which have matured during the past years, make monoliths interesting for an increasing number of biochemical and medical applications.

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“…For methods based on amplification [3], sequencing [4] or hybridization [5] it is necessary to isolate and concentrate DNA from a complex sample containing various classes of compounds. Traditional methods involving solvent precipitation are replaced by solid-phase extraction on silica or organic polymers [6, 7] or use of magnetic particles [8], which offer automation and miniaturization for applications where sample is available in limited quantity [6]. However, due to lack of selectivity, DNA extraction efficiency on silica or similar materials suffers from competing molecules like proteins that must be removed from the sample, increasing process complexity [9].…”

Section: Introductionmentioning

confidence: 99%

Sequence-specific DNA solid-phase extraction in an on-chip monolith: Towards detection of antibiotic resistance genes

Knob

Nelson

Robison

2017

Journal of Chromatography A

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Antibiotic resistance of bacteria is a growing problem and presents a challenge for prompt treatment in patients with sepsis. Currently used methods rely on culturing or amplification; however, these steps are either time consuming or suffer from interference issues. A microfluidic device was made from black polypropylene, with a monolithic column modified with a capture oligonucleotide for sequence selective solid-phase extraction of a complementary target from a lysate sample. Porous properties of the monolith allow flow and hybridization of a target complementary to the probe immobilized on the column surface. Good flow through properties enable extraction of a 100 μL sample and elution of target DNA in 12 minutes total time. Using a fluorescently labeled target oligonucleotide related to Verona Integron-Mediated Metallo-β-lactamase it was possible to extract and detect a 1 pM sample with 83% recovery. Temperature-mediated elution by heating above the duplex melting point provides a clean extract without any agents that interfere with base pairing, allowing various labeling methods or further downstream processing of the eluent. Further integration of this extraction module with a system for isolation and lysis of bacteria from blood, as well as combining with single-molecule detection should allow rapid determination of antibiotic resistance.

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Poly(hydroxyethyl methacrylate) based affinity cryogel for plasmid DNA purification

Cited by 60 publications

References 47 publications

Molecular imprinting based composite cryogel membranes for purification of anti-hepatitis B surface antibody by fast protein liquid chromatography

Molecular imprinting based composite cryogel membranes for purification of anti-hepatitis B surface antibody by fast protein liquid chromatography

Monolithic media for applications in affinity chromatography

Sequence-specific DNA solid-phase extraction in an on-chip monolith: Towards detection of antibiotic resistance genes

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