Heparin removal from human plasma using molecular imprinted cryogels

Baydemir, Gözde; Deni̇zli̇, Adil

doi:10.3109/21691401.2014.897631

Cited by 21 publications

(15 citation statements)

References 43 publications

(41 reference statements)

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“…The precomplex amount designates the amount of molecular cavities in the MIPs directly; because the protein C specific nanocavities were obtained after the removal of the protein C from the synthesized pcMIPs. If the precomplex amount is high, then the cavities come together and the combined cavities not allowed the optimum adsorption due to the lack of 3‐D recognition . Moreover, the synthesized pcMIPs were not stable in higher amounts of the precomplex in the polymer receipt.…”

Section: Methodsmentioning

confidence: 99%

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Protein C recognition by ion-coordinated imprinted monolithic cryogels

et al. 2017

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The protein C imprinted monolithic cryogel was synthesized using 2-hydroxyethyl methacrylate by redox cryo-polymerization method. The prepared monolithic cryogel was characterized by Fourier transform infrared spectroscopy, swelling test, surface area measurements, and scanning electron microscopy. The nonimprinted cryogel was prepared as well for control. Adsorption of protein C from aqueous solutions was investigated in a continuous mode and several parameters affecting adsorption performance were optimized. The maximum protein C adsorption amount was 30.4 mg/g. The selectivity studies were performed by monolithic column studies and fast protein liquid chromatography, using hemoglobin and human serum albumin as competing proteins. The relative selectivity coefficients were 2.37 and 8.89 for hemoglobin and human serum albumin, respectively. Reusability was tested for ten consecutive adsorption-desorption cycles, and no significant change in adsorption capacity was recorded. A pseudo-second-order model was suitable to interpret kinetic data, and the Langmuir model suited the adsorption isotherms well.

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Section: Methodsmentioning

confidence: 99%

“…The swelling tests were performed to characterize the water uptake behavior of the cryogels . The swelling ratios were detected by placing dried cryogel samples in 50 mL of water.…”

Section: Methodsmentioning

confidence: 99%

Protein C recognition by ion-coordinated imprinted monolithic cryogels

et al. 2017

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“…For example, Baydemir et al have synthesized heparin (Hp) molecular imprinted cryogels, in which N ‐[3‐dimethylamino)‐propyl] methacrylamide was the cationic functional monomer and heparin was the template. The results of selectivity studies showed that HpMIP column could successfully 90% of Hp from 28 U mL −1 of human plasma samples …”

Section: Biological Molecular Imprinting With Different Scalementioning

confidence: 99%

“…The results of selectivity studies showed that HpMIP column could successfully 90% of Hp from 28 U mL −1 of human plasma samples. [168] "Epitope" Imprinting: Epitope imprinting are introduced in detail in the abovementioned protein imprinting. [169,170] Owing to amphiphilic and high molecular weight of poly saccharides, it is difficult to prepare MIPs by using the entire poly saccharides as a template.…”

Section: Polysaccharides Imprintingmentioning

confidence: 99%

Molecularly Imprinted Materials for Selective Biological Recognition

Zhang

et al. 2019

Macromol. Rapid Commun.

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Molecular imprinting is an approach of generating imprinting cavities in polymer structures that are compatible with the target molecules. The cavities have memory for shape and chemical recognition, similar to the recognition mechanism of antigen–antibody in organisms. Their structures are also called biomimetic receptors or synthetic receptors. Owing to the excellent selectivity and unique structural predictability of molecularly imprinted materials (MIMs), practical MIMs have become a rapidly evolving research area providing key factors for understanding separation, recognition, and regenerative properties toward biological small molecules to biomacromolecules, even cell and microorganism. In this review, the characteristics, morphologies, and applicability of currently popular carrier materials for molecular imprinting, especially the fundamental role of hydrogels, porous materials, hierarchical nanoparticles, and 2D materials in the separation and recognition of biological templates are discussed. Moreover, through a series of case studies, emphasis is given on introducing imprinting strategies for biological templates with different molecular scales. In particular, the differences and connections between small molecular imprinting (bulk imprinting, “dummy” template imprinting, etc.), large molecular imprinting (surface imprinting, interfacial imprinting, etc.), and cell imprinting strategies are demonstrated in detail. Finally, future research directions are provided.

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“…Molecular imprinting (MI) method encompass design and synthesis of novel materials which are capable of recognizing proteins and other biological assemblies, such as whole cells and viruses and have potential fundamental and practical applications [10][11][12][13]. Specific applications of such materials include down-stream bioprocess for the purification of biopharmaceuticals, drug delivery, diagnostics, sensors, etc.…”

Section: Introductionmentioning

confidence: 99%

Controlled release of mitomycin C from PHEMAH–Cu(II) cryogel membranes

Bakhshpour

Yavuz

Deni̇zli̇

2018

Artificial Cells, Nanomedicine, and Biotechnology

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Molecular imprinting technique was used for the preparation of antibiotic and anti-neoplastic chemotherapy drug (mitomycin C) imprinted cryogel membranes (MMC-ICM). The membranes were synthezied by using metal ion coordination interactions with N-methacryloyl-(l)-histidine methyl ester (MAH) functional monomer and template molecules (i.e. MMC). The 2-hydroxyethyl methacrylate (HEMA) monomer and methylene bisacrylamide (MBAAm) crosslinker were used for the preparation of mitomycin C imprinted cryogel membranes by radical suspension polymerization technique. The imprinted cryogel membranes were characterized by scanning electron microscopy (SEM), Brunauer-Emmett-Teller (BET), Fourier transform infrared spectroscopy-attenuated total reflectance (FTIR-ATR) and swelling degree measurements. Cytotoxicity of MMC-ICMs was investigated using mouse fibroblast cell line L929. Time-dependent release of MMC was demonstrated within 150 h from cryogel membranes. Cryogels demonstrated very high MMC loading efficiency (70-80%) and sustained MMC release over hours.

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Heparin removal from human plasma using molecular imprinted cryogels

Cited by 21 publications

References 43 publications

Protein C recognition by ion-coordinated imprinted monolithic cryogels

Protein C recognition by ion-coordinated imprinted monolithic cryogels

Molecularly Imprinted Materials for Selective Biological Recognition

Controlled release of mitomycin C from PHEMAH–Cu(II) cryogel membranes

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