Selective monitoring of polynuclear aromatic hydrocarbons by high pressure liquid chromatography with a variable wavelength detector

Krstulović, Ante M.; Rosie, Douglas M.; Brown, Phyllis R.

doi:10.1021/ac50003a029

Cited by 82 publications

(13 citation statements)

References 16 publications

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“…Material, SRM 1649) and environmental tobacco smoke (ETS) were prepared in cyclohexane by [15][16][17][18][19][20][21][22][23][24][25][26][27][28][29][30] min sonication of the particles (5 mg and 2 mg respectively) and cleaned using silica SEP-PAK columns [21]. An aliquot of the cleaned extract in cyclohexane was diluted in an aliquot of tetrahydrofuran and three aliquots of acetonitrile before the HPLC analysis.…”

Section: Extracts Of Urban Dust (National Institute Of Standards and mentioning

confidence: 99%

Selective Fluorescence Detection of Polycyclic Aromatic Hydrocarbons in Environmental Tobacco Smoke and Other Airborne Particles

Mahanama

Gundel

Daisey

1994

International Journal of Environmental Analytical Chemistry

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Section: Extracts Of Urban Dust (National Institute Of Standards and mentioning

confidence: 99%

Selective Fluorescence Detection of Polycyclic Aromatic Hydrocarbons in Environmental Tobacco Smoke and Other Airborne Particles

Mahanama

Gundel

Daisey

1994

International Journal of Environmental Analytical Chemistry

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“…Hace un par de décadas, se discutía la utilización de HPLC como técnica para la detección de HAP debido a la baja resolución que poseían los equipos. Sin embargo, con los aparatos modernos se ha mejorado visiblemente su resolución, empleando detectores de FL, que son más sensibles que los que se empleaban anteriormente en los equipos de HPLC (luz UV) (por ejemplo: Dong et al 1976, Krstulovic et al 1976. De hecho, los nuevos equipos de cromatografía líquida superan en sensibilidad a los aparatos GC, aunque esto depende fuertemente de las características del equipo empleado y de la columna utilizada (Gratz et al 2000, Liaud et al 2015.…”

Section: Discussionunclassified

Método Optimizado Para La Determinación De Hidrocarburos Aromáticos Policíclicos Asociados a Partículas Atmosféricas

Tames

Busso

Carreras

2019

Rev. Int. Contam. Ambie.

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Palabras clave: contaminantes urbanos, cromatografía líquida de alto rendimiento, fluorescencia RESUMEN Los hidrocarburos aromáticos policíclicos son contaminantes ambientales ubicuos en el aire, derivados de la combustión incompleta de materia orgánica. Por sus características físicas y químicas, es frecuente encontrarlos asociados a partículas, favoreciendo su permanencia en la atmósfera. En las últimas décadas, el monitoreo de hidrocarburos aromáticos policíclicos en ambientes urbanos se ha convertido en una prioridad debido a la gran cantidad de individuos expuestos. En este trabajo se evaluaron modificaciones de una metodología estándar para la extracción, detección y cuantificación de hidrocarburos aromáticos policíclicos adsorbidos en partículas de 2.5 µm de diámetro aerodinámico. Estas variaciones permitieron reducir considerablemente los tiempos y costos de procesamiento, la detección y cuantificación, sin disminuir la sensibilidad para la detección de estos compuestos. Por otro lado, la reducción considerable en la cantidad de disolvente utilizado resulta en una metodología más amigable con el ambiente.

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“…It is possible that other polar metabolites do not exhibit UV absorption at 339 nm or at the chosen excitation/emission fluorescence wavelengths in the samples of N. diversicolor. Although the wavelength of 254 nm is better for PAH measurements in complex mixtures, higher‐molecular‐weight PAHs like pyrene have absorption maxima at higher wavelengths that can be utilized for more selective analysis [40]. Loss of aromaticity of one of the four rings with the formation of phenanthrene‐like compounds would, therefore, not be detected by the present method.…”

Section: Discussionmentioning

confidence: 99%

1‐hydroxypyrene glucuronide as the major aqueous pyrene metabolite in tissue and gut fluid from the marine deposit‐feeding polychaete Nereis diversicolor

Giessing

Mayer

Forbes

2003

Enviro Toxic and Chemistry

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Both 1-hydroxypyrene and 1-hydroxypyrene glucuronide are identified as the primary phase I and phase II metabolites of the four-ringed polycyclic aromatic hydrocarbon (PAH) pyrene in the marine deposit-feeding polychaete Nereis diversicolor. Identification of pyrene and primary metabolites was performed using high-pressure liquid chromatography (HPLC) with diode-array detection and fluorescence detection (HPLC/DAD/F) and an ion-trap mass spectrometer for positive identification of 1-hydroxypyrene glucuronide. Besides 1-hydroxypyrene and 1-hydroxypyrene glucuronide, the HPLC/F trace of tissue samples from pyrene-exposed worms showed three additional low-intensity peaks that may be related to pyrene metabolism based on similar excitation/emission wavelengths. The peaks were all too low in intensity to be positively identified. Of the total PAH in tissue, 1-hydroxypyrene glucuronide, 1-hydroxypyrene, and pyrene constituted 73%, 2%, and 25% respectively. Gut elimination of metabolic products is supported by the identification of 1-hydroxypyrene and 1-hydroxypyrene glucuronide in both gut fluid and defecation water. Being the only phase I metabolite of pyrene, 1-hydroxypyrene becomes a useful marker for PAH exposure, and it may serve as a valuable model compound for assessing species-specific PAH metabolic capabilities.

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Selective monitoring of polynuclear aromatic hydrocarbons by high pressure liquid chromatography with a variable wavelength detector

Cited by 82 publications

References 16 publications

Selective Fluorescence Detection of Polycyclic Aromatic Hydrocarbons in Environmental Tobacco Smoke and Other Airborne Particles

Selective Fluorescence Detection of Polycyclic Aromatic Hydrocarbons in Environmental Tobacco Smoke and Other Airborne Particles

Método Optimizado Para La Determinación De Hidrocarburos Aromáticos Policíclicos Asociados a Partículas Atmosféricas

1‐hydroxypyrene glucuronide as the major aqueous pyrene metabolite in tissue and gut fluid from the marine deposit‐feeding polychaete Nereis diversicolor

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