In preparing nucleotide extracts for HPLC analysis using microparticle, chemically bonded packings, tri-octyl-amine-freon solutions can be used for the extraction of the acid from the solution if the concentration of the amine is carefully controlled.
Nucleosides and bases in plasma and serum samples were identified using multiple or variable wavelength detectors by two techniques: absorbance ratios and the enzymatic peak shift. Compounds such as uric acid and xanthine, which fluoresce, were further identified using a fluorescence detector with an excitation wavelength of 325 nm and a cut-off filter at 340 nm. A peak which appeared in all the samples and was not a nucleic acid component was identified as tryptophan by using the fluorescence detector. These techniques can be used alone or In combination for peak Identification.
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