Extraction Procedures for Use Prior to HPLC Nucleotide Analysis Using Microparticle Chemically Bonded Packings

Chen, Show-Chuen; Brown, Phyllis R.; Rosie, Douglas M.

doi:10.1093/chromsci/15.6.218

Cited by 120 publications

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“…Metabolites from muscle sections were extracted in cold ethanolic (20% vol/vol) perchloric acid (3.5% wt/vol) (PCA) and neutralized with tri-n-octylamine and 1,1,2-trichlorotrifluoroethane (10). Perfusate and plasma samples were similarly extracted using PCA.…”

Section: Metabolite and Creatine Uptake Rate Analysesmentioning

confidence: 99%

Creatine uptake and creatine transporter expression among rat skeletal muscle fiber types

Brault

Terjung

2003

American Journal of Physiology-Cell Physiology

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Section: Metabolite and Creatine Uptake Rate Analysesmentioning

confidence: 99%

Creatine uptake and creatine transporter expression among rat skeletal muscle fiber types

Brault

Terjung

2003

American Journal of Physiology-Cell Physiology

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“…Metabolites from muscle sections were extracted in cold ethanolic (20% vol/vol) perchloric acid (PCA; 3.5% wt/vol) and neutralized with tri-n-octylamine and 1,1,2-trichloro-trifluoroethane (9). Perfusate, plasma, and powdered food samples were similarly extracted using PCA.…”

Section: Metabolite and Uptake Rate Analysesmentioning

confidence: 99%

Muscle creatine uptake and creatine transporter expression in response to creatine supplementation and depletion

Brault

Abraham

Terjung

2003

Journal of Applied Physiology

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The total creatine pool size [Crtotal; creatine (Cr) + phosphocreatine (PCr)] is crucial for optimal energy utilization in skeletal muscle, especially at the onset of exercise and during intense contractions. The Crtotal likely is controlled by long-term modulation of Cr uptake via the sodium-dependent Cr transporter (CrT). To test this hypothesis, adult male Sprague-Dawley rats were fed 1% Cr, their muscle Crtotal was reduced by ∼85% [1% β-guanidinoproprionic acid (β-GPA)], or their muscle Crtotal was repleted (1% Cr after β-GPA depletion). Cr uptake was assessed by skeletal muscle 14C-Cr accumulation to Cr and PCr by using hindlimb perfusion, and CrT protein content was assessed by Western blot. Cr uptake rate decreased with dietary Cr supplementation in the white gastrocnemius (WG; 45%) only. Depletion of muscle Crtotal to ∼15% of normal increased Cr uptake in the soleus (21%) and red gastrocnemius (22%), corresponding to 70–150% increases in muscle CrT content. In contrast, the inherently lower Cr uptake rate in the WG was unchanged with depletion of muscle Crtotal even though CrT band density was increased by 230%. Thus there was no direct relationship between apparent muscle CrT abundance and Cr uptake rates. However, Cr uptake rates scaled inversely with decreases in muscle Crtotal in the high-oxidative muscle types but not in the WG. This implies that factors controlling Cr uptake are different among fiber types. These observations may help explain the influence of initial muscle Crtotal, time dependency, and variations in muscle Crtotal accumulation during Cr supplementation.

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“…To evaluate the physiological significance of these observations, nucleotide pools were measured by the method of Chen et al (3) and quantitated by comparative integration with known standard samples ( Table 2). The various enteric strains differred slightly in the levels of endogenous nucleotide pools and could be divided into three groups: (i) E. coli and Yersinia intermedia 48 had equivalent pool levels; (ii) Yersinia fredriksenii pools were approximately 50% higher than those of E. coli; and (iii) the nucleotide pools of P. vulgaris, S. marcescens, E. herbicola, Yersinia enterocolitica, and Yersinia kristensenii were 30 to 70% lower than those of E. coli.…”

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ATP-liganded form of aspartate transcarbamoylase, the logical regulatory target for allosteric control in divergent bacterial systems

1988

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In Escherichia coli, the mechanism for regulatory control of aspartate transcarbamoylase is clear; CTP allosterically inhibits catalysis in direct competition with ATP. However, both CTP and ATP may be activators or may have no effect on aspartate transcarbamoylases from other enteric bacteria. A common regulatory logic observed was that the ATP-activated enzymes were rendered less active as the result of competition with CTP, regardless of the independent effects.

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Extraction Procedures for Use Prior to HPLC Nucleotide Analysis Using Microparticle Chemically Bonded Packings

Abstract: In preparing nucleotide extracts for HPLC analysis using microparticle, chemically bonded packings, tri-octyl-amine-freon solutions can be used for the extraction of the acid from the solution if the concentration of the amine is carefully controlled.

Cited by 120 publications

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Creatine uptake and creatine transporter expression among rat skeletal muscle fiber types

Creatine uptake and creatine transporter expression among rat skeletal muscle fiber types

Muscle creatine uptake and creatine transporter expression in response to creatine supplementation and depletion

ATP-liganded form of aspartate transcarbamoylase, the logical regulatory target for allosteric control in divergent bacterial systems

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