Selective capture of human red blood cells based on blood group using long-range surface plasmon waveguides

Quantitative blood group typing using surface plasmon resonance

“…It is a label-free optical technique that can monitor intermolecular binding events in real time. SPR relies on a polarized laser to measure the change of adsorbed mass on a metal chip surface by monitoring the change in refractive index (RI), and reporting the change as a binding response unit (RU) (Krupin et al, 2014). However, SPR investigations of whole cell interactions are significantly less common (Stojanović et al, 2014).…”

Surface Plasmon Resonance: Advances of Label-Free Approaches in The Analysis of Biological Samples

“…The selective binding of a cell surface antigen to the immobilized antibody was used in the study by Krupin et al [49] to detect human red blood cells. A biosensor based on LRSP consisted of gold straight waveguides embedded in amorphous fluoropolymer CYTOP with an etched fluidic channel; the gold waveguides were functionalized with antibodies against antigen A (blood group A) by forming a self-assembled monolayer of 16-mercaptohexadecanoic acid followed by anti-A IgG conjugation.…”

Long-range surface plasmons on gold-coated single-mode fibers

“…LRSPPs are less confined and less surface sensitive but their attenuation can be one to three orders of magnitude lower, depending on the structure, leading to significantly longer optical interaction lengths, or narrower resonances under wavelength interrogation, which ultimately produces a sensor with greater transducer (overall sensitivity [11]. Indeed, LRSPPs have been successfully used in several biosensing demonstrations in prism-coupled [12][13][14][15][16] and waveguide geometries [17][18][19][20].…”