Surface Plasmon Resonance: Advances of Label-Free Approaches in The Analysis of Biological Samples

Riedel, Tomáš; Májek, Pavel; Rodriguez‐Emmenegger, Cesar; Brynda, Eduard

doi:10.4155/bio.14.246

Cited by 17 publications

(4 citation statements)

References 73 publications

(70 reference statements)

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“…The vast majority of conventional lead compound discovery techniques demand time-consuming, extensive effectiveness verification. Our team used SPR to evaluate the binding affinity of inhibitors to target proteins, combining the advantages of this technique, which include accuracy, efficiency, and the need for minimal assay material, for the effective evaluation of compounds and highly automated large-scale assays during the pre-drug screening stage [50,51]. Furthermore, the SPR instrument's varied properties allowed us to investigate the affinity of the same target proteins for different types of TCM metabolites.…”

Section: Introductionmentioning

confidence: 99%

Detection of Various Traditional Chinese Medicinal Metabolites as Angiotensin-Converting Enzyme Inhibitors: Molecular Docking, Activity Testing, and Surface Plasmon Resonance Approaches

Wu,

Jiao,

Luo

et al. 2023

Molecules

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Angiotensin-converting enzyme 1 (ACE1) is a peptide involved in fluid and blood pressure management. It regulates blood pressure by converting angiotensin I to angiotensin II, which has vasoconstrictive effects. Previous studies have shown that certain compounds of natural origin can inhibit the activity of angiotensin-converting enzymes and exert blood pressure-regulating effects. Surface Plasmon Resonance (SPR) biosensor technology is the industry standard method for observing biomolecule interactions. In our study, we used molecular simulation methods to investigate the docking energies of various herbal metabolites with ACE1 proteins, tested the real-time binding affinities between various herbal metabolites and sACE1 by SPR, and analyzed the relationship between real-time binding affinity and docking energy. In addition, to further explore the connection between inhibitor activity and real-time binding affinity, several herbal metabolites′ in vitro inhibitory activities were tested using an ACE1 activity test kit. The molecular docking simulation technique’s results and the real-time affinity tested by the SPR technique were found to be negatively correlated, and the virtual docking technique still has some drawbacks as a tool for forecasting proteins′ affinities to the metabolites of Chinese herbal metabolites. There may be a positive correlation between the enzyme inhibitory activity and the real-time affinity detected by the SPR technique, and the results from the SPR technique may provide convincing evidence to prove the interaction between herbal metabolites and ACE1 target proteins.

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Section: Introductionmentioning

confidence: 99%

Detection of Various Traditional Chinese Medicinal Metabolites as Angiotensin-Converting Enzyme Inhibitors: Molecular Docking, Activity Testing, and Surface Plasmon Resonance Approaches

Wu,

Jiao,

Luo

et al. 2023

Molecules

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“…Surface fouling caused by the adhesion of proteins, peptides, lipids and microorganisms is a major challenge that limits the performance of various medical devices, including diagnostic and biosensing platforms [ 1 ], tissue engineering constructs [ 2 ], and blood-contacting devices [ 3 ]. The interactions pertaining to the nonspecific adsorption of proteins include hydrophobic effects, van der Waals and coulumbic forces [ 4 , 5 ]. For example, biointerfaces for the capture and release of biological targets, such as circulating cancer cells (CTCs) [ 6 ] and extracellular vesicles (EVs) [ 7 ], are affected by protein fouling which results in obstruction of capture probes and contamination by proteins [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

Conducting Polymer-Infused Electrospun Fibre Mat Modified by POEGMA Brushes as Antifouling Biointerface

et al. 2022

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Biofouling on surfaces, caused by the assimilation of proteins, peptides, lipids and microorganisms, leads to contamination, deterioration and failure of biomedical devices and causes implants rejection. To address these issues, various antifouling strategies have been extensively studied, including polyethylene glycol-based polymer brushes. Conducting polymers-based biointerfaces have emerged as advanced surfaces for interfacing biological tissues and organs with electronics. Antifouling of such biointerfaces is a challenge. In this study, we fabricated electrospun fibre mats from sulphonated polystyrene-block-poly(ethylene-ran-butylene)-block-polystyrene (sSEBS), infused with conducting polymer poly(3,4-ethylenedioxythiophene) (PEDOT) (sSEBS-PEDOT), to produce a conductive (2.06 ± 0.1 S/cm), highly porous, fibre mat that can be used as a biointerface in bioelectronic applications. To afford antifouling, here the poly(oligo (ethylene glycol) methyl ether methacrylate) (POEGMA) brushes were grafted onto the sSEBS-PEDOT conducting fibre mats via surface-initiated atom transfer radical polymerization technique (SI-ATRP). For that, a copolymer of EDOT and an EDOT derivative with SI-ATRP initiating sites, 3,4-ethylenedioxythiophene) methyl 2-bromopropanoate (EDOTBr), was firstly electropolymerized on the sSEBS-PEDOT fibre mat to provide sSEBS-PEDOT/P(EDOT-co-EDOTBr). The POEGMA brushes were grafted from the sSEBS-PEDOT/P(EDOT-co-EDOTBr) and the polymerization kinetics confirmed the successful growth of the brushes. Fibre mats with 10-mers and 30-mers POEGMA brushes were studied for antifouling using a BCA protein assay. The mats with 30-mers grafted brushes exhibited excellent antifouling efficiency, ~82% of proteins repelled, compared to the pristine sSEBS-PEDOT fibre mat. The grafted fibre mats exhibited cell viability >80%, comparable to the standard cell culture plate controls. Such conducting, porous biointerfaces with POEGMA grafted brushes are suitable for applications in various biomedical devices, including biosensors, liquid biopsy, wound healing substrates and drug delivery systems.

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“…Fouling consists of nonspecic protein adsorption which occurs in almost any articial surface caused by the interplay of different forces (coulombic, hydrophobic effect, and van der Waals) acting between proteins and the surface of the material. 1 The formation of a conditioning lm promotes the adhesion of platelets which by generation of thrombin initiate blood coagulation. In addition, the adsorption of brinogen, and even serum albumin, in a non-natural conformation causes the initiation of the coagulation cascade.…”

Section: Introductionmentioning

confidence: 99%

Total removal of intact blood plasma proteins deposited on surface-grafted polymer brushes

Riedel

Májek

Riedelová-Reicheltová

et al. 2016

Anal. Methods

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Surface Plasmon Resonance: Advances of Label-Free Approaches in The Analysis of Biological Samples

Cited by 17 publications

References 73 publications

Detection of Various Traditional Chinese Medicinal Metabolites as Angiotensin-Converting Enzyme Inhibitors: Molecular Docking, Activity Testing, and Surface Plasmon Resonance Approaches

Detection of Various Traditional Chinese Medicinal Metabolites as Angiotensin-Converting Enzyme Inhibitors: Molecular Docking, Activity Testing, and Surface Plasmon Resonance Approaches

Conducting Polymer-Infused Electrospun Fibre Mat Modified by POEGMA Brushes as Antifouling Biointerface

Total removal of intact blood plasma proteins deposited on surface-grafted polymer brushes

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