Quantitative blood group typing using surface plasmon resonance

Then, Whui Lyn; Aguilar, Marie Isabel; Garnier, Gil

doi:10.1016/j.bios.2015.05.053

Cited by 23 publications

(19 citation statements)

References 13 publications

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“…2). This is consistent with the previous study, where ‘normal’ D antigens on donor cells were detected between a range of 530–1200 RU 27 . We propose that responses below 600 RU can be considered weak.…”

Section: Resultssupporting

confidence: 93%

“…In our previous study, the concept of using a generic anti-human IgG antibody to detect antigen positive RBCs was explored using the D antigen as an example 27 . The concept draws from the Indirect Antiglobulin Test (IAT) used to detect blood groups using IgG antibodies with the CAT 3 .…”

Section: Introductionmentioning

confidence: 99%

“…However, sensitivity of this new method for weak and partial antigen detection has not yet been explored 27 . Here, we optimize and validate this new technique using human-sourced donor blood samples to detect the weakest expressions of the D antigen 27 .…”

Section: Introductionmentioning

confidence: 99%

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Quantitative Detection of Weak D Antigen Variants in Blood Typing using SPR

Then

Aguilar

Garnier

2017

Sci Rep

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Modern techniques for quantifying blood group antibody-antigen interactions are very limited, especially for weaker interactions which result from low antigen expression and/or partial expression of the antigen structure. Surface plasmon resonance (SPR) detection is often used to monitor and quantify bio-interactions. Previously, a regenerable, multi-fucntional platform for quantitative RBC phenotyping of normal antigen expression using SPR detection was reported. However, detection of weaker variants were not explored. Here, this sensitivity study used anti-human IgG antibodies immobilized to a gold sensor surface to two clinically important types of weaker D variants using SPR; weak D and partial D. Positive pre-sensitised cells bind to the anti-human IgG monolayer, and the response unit (RU) is reported (>100 RU). Unbound negative cells are directly eluted (<100 RU). Weak D cells were detected between a range of 180–580 RU, due to a lower expression of antigens. Partial D cells, category D VI, were also positively identified (352–1147 RU), similar to that of normal D antigens. The detection of two classes of weaker D variants was achieved for the first time using this fully regenerable SPR platform, opening up a new avenue to replace the current subjective and arbitrary methods for quantifying blood group antibody-antigen interactions.

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Section: Resultssupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

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Quantitative Detection of Weak D Antigen Variants in Blood Typing using SPR

Then

Aguilar

Garnier

2017

Sci Rep

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“…Surface plasmon resonance and quartz crystal microbalance (QCM) demonstrated multi-antigen detection, high sensitivity, and quantification performance; however, the regenerating property and sample analysis capacity are not satisfactory. 15,16 Therefore, a novel quantitative and multiplex detection method is needed.…”

Section: Introductionmentioning

confidence: 99%

Quantitative and multiplexed detection for blood typing based on quantum dot–magnetic bead assay

Zhang

Fan

et al. 2017

IJN

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Background Accurate and reliable blood grouping is essential for safe blood transfusion. However, conventional methods are qualitative and use only single-antigen detection. We overcame these limitations by developing a simple, quantitative, and multiplexed detection method for blood grouping using quantum dots (QDs) and magnetic beads. Methods In the QD fluorescence assay (QFA), blood group A and B antigens were quantified using QD labeling and magnetic beads, and the blood groups were identified according to the R value (the value was calculated with the fluorescence intensity from dual QD labeling) of A and B antigens. The optimized performance of QFA was established by blood typing 791 clinical samples. Results Quantitative and multiplexed detection for blood group antigens can be completed within 35 min with more than 10 5 red blood cells. When conditions are optimized, the assay performance is satisfactory for weak samples. The coefficients of variation between and within days were less than 10% and the reproducibility was good. The ABO blood groups of 791 clinical samples were identified by QFA, and the accuracy obtained was 100% compared with the tube test. Receiver-operating characteristic curves revealed that the QFA has high sensitivity and specificity toward clinical samples, and the cutoff points of the R value of A and B antigens were 1.483 and 1.576, respectively. Conclusion In this study, we reported a novel quantitative and multiplexed method for the identification of ABO blood groups and presented an effective alternative for quantitative blood typing. This method can be used as an effective tool to improve blood typing and further guarantee clinical transfusion safety.

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“…However, while the agglutination‐based techniques seem to have reached their full potential, solid‐phase testing can be applied to test a donor sample for multiple parameters simultaneously. By immobilizing blood group–specific antibodies, binding of RBCs can be monitored in microfluidic chambers by imaging techniques and as well by surface plasmon resonance (SPR) …”

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confidence: 99%

Multiplex blood group typing by cellular surface plasmon resonance imaging

et al. 2018

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BACKGROUND: Blood-group typing of donors and patients is essential to avoid incompatible transfusions. Transfusion of incompatible RBCs may result in alloimmunization complicating future transfusions or in the presence of antibodies in adverse reactions. With more than 300 blood group antigens identified, it is difficult to provide fully compatible blood. Currently, standard practice is to match for the most immunogenic antigens. While the current agglutination-based RBC-typing methods are reliable for testing a selected number of antigens, they are not easily adaptable for high-throughput multiplex blood typing beyond the current standard.STUDY DESIGN AND METHODS: Surface plasmon resonance (SPR) is a label-free method to follow molecular-and, very recently, also cellular-interactions in real time. Demonstration of binding of RBCs to blood group antigen-specific antibodies by SPR has already been achieved. Here, we demonstrate the generation of an SPR array equipped with clinically relevant blood group antibodies (A, B, and Rh blood groups). To validate this method, we blindly compared typing of 946 blood donors with results of current diagnostic agglutination-based methods.RESULTS: RBC typing was achieved by monitoring RBC binding to blood group-specific antibodies on the sensor simultaneously within 5 minutes per sample. Regeneration of the chip was robust, allowing for typing of at least 100 samples. The typing results gave a 100% match with classical serology with all antibodies tested besides anti-E/e monoclonals, which gave inconsistent results due to low antibody specificity.CONCLUSION: This study demonstrates that SPRbased RBC typing for multiple antigens can be realized simultaneously with high-quality antibodies, enabling reduced hands-on time and possibly improving cost efficiency.I ncompatible blood transfusion can lead to transfusion reactions or to immunization of the recipient, who generates antibodies that can lead to serious complications in subsequent transfusions. In the case of preexisting alloantibodies, blood transfusion can therefore be given only with RBCs negative for the corresponding blood group antigen. To date, more than 300 blood group antigens have been identified in 36 blood group systems, 1 with varying clinical significance. 2,3 Typing is standardly provided for only limited blood groups with naturally occurring anti-A and B antibodies in antigen-negative individuals, and for the highly immunogenic D antigen. These are universally accepted as important steps in pretransfusion testing. 4 Limited phenotype-matched blood transfusion (including C, ABBREVIATIONS: cSPRi = cellular surface plasmon resonance imaging; S = sedimentation response value; SPR = surface plasmon resonance; SPRi = surface plasmon resonance imaging; T = total response. From the c, E, e, and K antigens) is also recommended in some countries for women during and before childbearing years, to prevent formation of antibodies potentially causing hemolytic disease of the fetus and newborn. 5 Extended phenotype-matched ...

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Quantitative blood group typing using surface plasmon resonance

Cited by 23 publications

References 13 publications

Quantitative Detection of Weak D Antigen Variants in Blood Typing using SPR

Quantitative Detection of Weak D Antigen Variants in Blood Typing using SPR

Quantitative and multiplexed detection for blood typing based on quantum dot–magnetic bead assay

Multiplex blood group typing by cellular surface plasmon resonance imaging

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Quantitative blood group typing using surface plasmon resonance

Cited by 23 publications

References 13 publications

Quantitative Detection of Weak D Antigen Variants in Blood Typing using SPR

Quantitative Detection of Weak D Antigen Variants in Blood Typing using SPR

Quantitative and multiplexed detection for blood typing based on quantum dot&ndash;magnetic bead assay

Multiplex blood group typing by cellular surface plasmon resonance imaging

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Quantitative and multiplexed detection for blood typing based on quantum dot–magnetic bead assay