Selection of strains for shiitake production in axenic substrate

Abstract

Shiitake mushroom consumption is increasing in Brazil. In addition to the implementation of new production methods, it is also important to increase productivity, quality and reduce production costs. In this study, six commercial Lentinula edodes strains were characterized for genetic diversity (rep-PCR analysis) and mushroom production (yield, number and weight of individual mushrooms) using different substrates and cultural conditions. All strains showed genetic differences by repetitive element palindromic based-polymerase chain reaction (rep-PCR). The richest substrate resulted in the greatest production under both environmental conditions. Strains LE4 and LE6 produced the majority of their mushrooms earlier than the other strains. The highest number of mushrooms was observed in the LE6 strain while the highest weights of individual mushrooms were observed in the LE4 strain. Controlled environmental conditions resulted in superior production for all strains, except for LE4, which had empirically greater yield in the semi-controlled environmental condition.

Enzyme activity and biochemical changes during production of Lentinula edodes (Berk.) Pegler

“…Philippoussis et al (2003) tested three formulations of substrates containing increasing concentrations of hemicellulose for L. edodes cultivation and found faster growth and early fruiting in the two substrates with highest content of hemicellulose. Zied et al (2016) reported higher mushroom yield when the sawdust-based substrate was supplemented with diverse kinds of bran or meal. Those supplements likely enriched the substrate with hemicellulose and soluble sugars, besides nitrogen, vitamins, amino acids, etc.…”

“…Six strains of Lentinula edodes (UFLA-LE1, UFLA-LE2, UFLA-LE3, UFLA-LE4, UFLA-LE5 and UFLA-LE6) as described by Zied et al (2016) were utilized. The stock cultures were maintained on malt agar medium (15.0 g L -1 of glucose; 15.0 g L -1 of malt extract; 15.0 g L -1 of agar) and incubated at 25 °C.…”

“…The substrate for the axenic cultivation of L. edodes was prepared as described by Zied et al (2016).…”

“…Those supplements likely enriched the substrate with hemicellulose and soluble sugars, besides nitrogen, vitamins, amino acids, etc. We used the same richer substrate reported by Zied et al (2016).…”

“…Shiitake mushrooms have been traditionally cultivated on logs of various species of trees such as oak and eucalyptus. However, the axenic cultivation on synthetic substrates, based upon different agricultural residues, has been gaining popularity in commercial production because the time to harvest is faster and productivity is much higher (Philippoussis et al, 2007;Zied et al, 2016).…”