Seleção de matrizes e clones de mangabeira para o cultivo in vitro

Machado, Luana de Lima; Ramos, Maria Lucrécia Gerosa; Caldas, L. S.; Vivaldi, Lúcio José

doi:10.1590/s0100-204x2004000500004

Cited by 12 publications

(11 citation statements)

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(1 reference statement)

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“…Lemos (2003) alcançou 62% em meio MS sem reguladores de crescimento e Sousa et al (2005), 70% de germinação em meio básico MS sólido ou líquido, independente de apresentarem ou não o tegumento. Machado et al (2004), avaliando o potencial de propagação in vitro de 11 matrizes de mangabeira nativas do Cerrado, observaram 92,4% de germinação em meio MS sem reguladores de crescimento, não se verificando diferenças entre as matrizes.…”

Section: Figura 1 Fases Da Germinação In Vitro De Mangabeiraunclassified

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Crescimento inicial de mangabeira (Hancornia speciosa Gomes) em diferentes meios de germinação in vitro

et al. 2007

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RESUMOO objetivo do trabalho foi determinar as condições mais favoráveis para a germinação in vitro de sementes e o crescimento inicial de plântulas de mangabeira. Após assepsia, sementes oriundas de frutos maduros foram inoculadas em tubos de ensaio contendo os seguintes tratamentos: T1-15 mL de meio de cultura MS; T2-15 mL de meio de cultura MS + 2,0 g L -1 de carvão ativado; T3-15 mL de meio de cultura ½ MS; e T4-15 mL de meio de cultura ½ MS + 2,0 g L -1 de carvão ativado. Todos os meios de cultura foram gelificados com 0,3 g L -1 de Phytagel® e suplementados com 3,0 g L -1 de sacarose. O experimento foi instalado em delineamento inteiramente casualizado com quatro tratamentos e oito repetições, sendo cada parcela experimental composta de dez tubos de ensaio contendo uma semente cada. Não houve diferença significativa dos tratamentos para a porcentagem de germinação aos 20 dias, que variou de 95 a 100%. Quanto ao comprimento da raiz principal, observou-se que o meio de cultura constituído de ½ MS com 2,0 g L -1 de carvão ativado proporcionou maior crescimento quando comparado com os demais tratamentos. Aos 50 dias, não foi observada a formação de plântulas anormais e nem diferenças significativas do comprimento da parte aérea das plântulas. Entretanto, a diluição em 50% dos sais do meio MS associada à presença de carvão ativado induziu maior crescimento da raiz principal (8,50 cm) quando comparado com meio MS, na presença (6,19 cm) ou ausência (6,00 cm) de carvão ativado. Termos para indexação:Propagação in vitro, meio de cultura, sementes, Apocynaceae. ABSTRACTThe objective of this study was to determine the most favorable conditions for the in vitro germination of mangaba seeds and initial development of plantlets. After asepsis, emerging seeds of mature fruits were inoculated in tubes contend the next treatments: T1-15 mL of MS culture medium; T2-15 mL of MS culture medium + 2.0 g L-1 of activated charcoal; T3-15 mL of ½ MS culture medium; and T4-15 mL of ½ MS culture medium + 2.0 g L-1 of activated charcoal. All the culture medium were gellified with 0.3 g L-1 of Phytagel® and supplemented with 3.0 g L-1 of sucrose. The statistical design was completely randomized with four treatments, eight repetitions and ten seeds by experimental unit. There was not significant difference of the treatments for the germination percentage at twenty days, which varied from 95 to 100%. The ½ MS with 2.0 g L-1 of activated charcoal promoted higher growth the main root when compared with the others treatments. After 50 days, abnormal plantlets were not observed and neither significant difference were verified among the length of the aerial part. However, the dilution in 50% of the MS culture medium associate to the presence of activated charcoal induced higher growth of the main root (8.50 cm) when compared with MS culture medium in the presence (6.19 cm) or absence (6.00 cm) of activated charcoal.

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Section: Figura 1 Fases Da Germinação In Vitro De Mangabeiraunclassified

“…Diversos trabalhos com a germinação in vitro de mangabeira, variedades botânicas do Nordeste e dos Cerrados, foram realizados (GRIGOLLETO, 1997;LEMOS et al, 2006;MACHADO et al, 2004). Entretanto, aspectos sobre o crescimento inicial in vitro da parte aérea e sistema radicular necessitam ser melhor caracterizados.…”

Section: Introductionunclassified

Crescimento inicial de mangabeira (Hancornia speciosa Gomes) em diferentes meios de germinação in vitro

et al. 2007

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“…This rich biodiversity occupies an area over 2 million km 2 , and represents about 23% of the Brazilian national territory (Klink & Machado, 2005). Several plant species of economic importance are found in this biome and many of them, if exploited consciously, have potential for the sustainable production of food and medicine (Machado, Ramos, Caldas, & Vivaldi, 2004).…”

Section: Introductionmentioning

confidence: 99%

<b>Behavior of lateral buds of <i>Hancornia speciosa</i> after cryopreservation by encapsulation-vitrification

Prudente

Paiva

Nery

et al. 2017

Acta Sci. Biol. Sci.

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ABSTRACT. Hancornia speciosa is a fruitful species from Cerrado biome with high economic potential. However, the intense and disordered extractivism have caused a reduction of its population in its endemic area. In addition, seed recalcitrance negatively affects the conventional conservation of the species. Aiming to find alternatives that enable the long-term conservation of this species, the study's objective was to assess the behavior of lateral bud's regrowth after cryopreservation procedures by encapsulation-vitrification technique. Sodium alginate capsules containing lateral buds were pre-cultured in liquid WPM supplemented with 1.0 M glycerol, and subsequently exposed to different concentrations of sucrose (0.3; 0.75 and 1.0 M) for 24 or 48 hours. The capsules were subjected to dehydration in silica gel or airflow hood for 0, 1, 2 and 3 hours before different incubation times in PVS2 (0, 15, 30, 60 and 120 minutes) at 0°C. A high regeneration percentage of lateral buds was observed after cryopreservation of capsules treated with 0.75 M sucrose plus 1.0 M glycerol (24 hours), associated with dehydration in an airflow hood (1 hour) and immersion in PVS2 (15 minutes). Encapsulation-vitrification allowed the long-term conservation, and provided high plant material survival rates after cryopreservation of Hancornia speciosa sensitive explants.Keywords: Mangabeira, recalcitrant species, long-term conservation, dehydration.Comportamento de gemas laterais de Hancornia speciosa após criopreservação por encapsulamento-vitrificação RESUMO. Hancornia speciosa é uma frutífera do Cerrado com elevado potencial econômico. Entretanto, o extrativismo desordenado causou a redução populacional em sua área endêmica. Além disso, a recalcitrância da semente afeta negativamente sua conservação convencional. Buscando alternativas de conservação para essa espécie, objetivou-se avaliar a regeneração das gemas laterais após a técnica de encapsulamento-vitrificação. Cápsulas de alginato de sódio contendo gemas laterais foram pré-cultivadas em meio WPM acrescido de 1,0 M de glicerol e, posteriormente, imersas em diferentes concentrações de sacarose (0,3; 0,75 e 1,0 M) por 24 ou 48 horas. As cápsulas foram submetidas à desidratação em sílica gel ou em fluxo laminar por 0, 1, 2 e 3 horas antes de sua incubação em diferentes tempos de PVS2 (0, 15, 30, 60 e 120 minutos). Elevada porcentagem de regeneração de gemas laterais foi observada após a criopreservação de cápsulas tratadas com 0,75 M de sacarose + 1,0 M de glicerol por 24 horas, associado com a desidratação em fluxo laminar (1 hora) e imersão em PVS2 (15 minutos). O encapsulamento-vitrificação é eficiente para a conservação de longo prazo e permite a obtençao de altas taxas de sobrevivência após a criopreservação de explantes sensíveis (gemas laterais) de Hancornia speciosa.Palavras-chave: Mangabeira, espécie recalcitrante, conservação em longo prazo, desidratação.

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“…It has a wide geographic distribution covering the territory from the State of Amapá to the State of São Paulo (MACHADO et al, 2004). It holds great economical potential because there are several possibilities to use their fruits to produce syrups, juices, pastries, ice cream, and jams (SOARES et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

“…Asexual propagation techniques have represented an important tool to overcome natural obstacles underlying vegetal species enabling the commercial cultivation in a shorter time and producing disease-free plants (MACHADO et al, 2004). The micropropagation is one of these asexual (PEREIRA et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Indirect in vitro organogenesis of Hancornia speciosa Gomes

Prudente¹,

Paiva²,

Nery³

et al. 2016

Biosci. J.

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ABSTRACT:Hancornia speciosa Gomes is a fruit species belonging to the Apocynaceae family and holds social, cultural and economic potential mostly due to its use in composition of many food industry products and the consumption its fruits in natura. Several aspects regarding their propagation need further studies, since the species is undergoing a continuous process of domestication. The objective was to obtain an in vitro protocol for indirect organogenesis and rooting with subsequent acclimatization of H. speciosa plants. To obtain indirect organogenesis, internodal segments were inoculated in WPM culture medium gelled with 7 g L -1 agar, added with 30 g L -1 sucrose, 0.4 g L -1 PVP, and supplied with different concentrations of 2,4-D (0.0; 2.46; 7.38; 12.30; and 17.22 µM), BAP (0.0; 4.92; 9.84; 14.76; and 19.68 µM), and TDZ (0.0; 4.92; 9.84; 14.76; and 19.68 µM). For the in vitro rooting, shootings with approximately 6.0 cm diameter length kept for 15 days in WPM medium with no plant growth regulator and, afterwards, subjected to treatments with different auxins (Control; 9.84 µM IBA; 9.84 µM NAA; and 9.84 µM IAA) as well as the combination among them, in order to verify their effects on percentage of rooting (%), number of roots, and average length of the largest root (cm). The formation of calluses was observed in all explants subjected to the concentrations of the regulators tested. The highest shooting regeneration occurred with 7.38 µM 2,4-D (73%). The highest percentage of shoot rooting (80%) and roots with the largest length (1.3 cm) were found in the culture medium with the combination of 4.92 µM NAA and 4.92 µM IBA. The in vitro regeneration of H. speciosa is feasible. The acclimatization of rooted shoots in Trospstrato® was accomplished with successful and 100% survival of plant material was observed during this stage.

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Seleção de matrizes e clones de mangabeira para o cultivo in vitro

Cited by 12 publications

References 1 publication

Crescimento inicial de mangabeira (Hancornia speciosa Gomes) em diferentes meios de germinação in vitro

Crescimento inicial de mangabeira (Hancornia speciosa Gomes) em diferentes meios de germinação in vitro

<b>Behavior of lateral buds of <i>Hancornia speciosa</i> after cryopreservation by encapsulation-vitrification

Indirect in vitro organogenesis of Hancornia speciosa Gomes

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