Seeding decellularized nerve allografts with adipose-derived mesenchymal stromal cells: An in vitro analysis of the gene expression and growth factors produced

Rbia, Nadia; Bulstra, Liselotte F.; Lewallen, Eric A.; Hovius, Steven E.R.; Wijnen, André J. van; Shin, Alexander Y.

doi:10.1016/j.bjps.2019.04.014

Cited by 25 publications

(20 citation statements)

References 57 publications

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“…Gene expression analyses have indicated that the neurotrophic markers NGF and GCNF are downregulated when ASCs have been seeded on a decellularized (motor) nerve graft, suggesting that upon adhesion to the substrate, ASCs become less involved in sensory nerve growth. In contrast, all myelination and angiogenesis markers are increased compared to control cells (Rbia, et al., 2019). Avascular nerve scaffolds seeded with ASCs have also been used to bridge the gap between the contralateral C7 nerve root and C5–6 nerve in a rat brachial plexus injury model.…”

Section: Acellular Nerve Scaffolds and Adipose Tissue Stem Cellsmentioning

confidence: 99%

Adipose tissue stem cells in peripheral nerve regeneration—In vitro and in vivo

Rhode

Beier

Ruhl

2020

J of Neuroscience Research

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Section: Acellular Nerve Scaffolds and Adipose Tissue Stem Cellsmentioning

confidence: 99%

Adipose tissue stem cells in peripheral nerve regeneration—In vitro and in vivo

Rhode

Beier

Ruhl

2020

J of Neuroscience Research

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“…Additional techniques for enhancing axons regeneration through long allografts grafts include filling them with platelet-rich plasma (PRP) [140], autologous Schwann cells [141,142], adipose-derived mesenchymal stem cells (ADSCs), or primary Schwann cell-like differentiated bone marrow-derived mesenchymal stem cells (DMSCs) [142][143][144][145]. These cells induce enhanced axon regeneration by releasing neurotrophic factors [146,147].…”

Section: Enhancing the Regeneration-promoting Capacity Of Allograftsmentioning

confidence: 99%

Restoration of Neurological Function Following Peripheral Nerve Trauma

Kuffler

Foy

2020

IJMS

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Following peripheral nerve trauma that damages a length of the nerve, recovery of function is generally limited. This is because no material tested for bridging nerve gaps promotes good axon regeneration across the gap under conditions associated with common nerve traumas. While many materials have been tested, sensory nerve grafts remain the clinical “gold standard” technique. This is despite the significant limitations in the conditions under which they restore function. Thus, they induce reliable and good recovery only for patients < 25 years old, when gaps are <2 cm in length, and when repairs are performed <2–3 months post trauma. Repairs performed when these values are larger result in a precipitous decrease in neurological recovery. Further, when patients have more than one parameter larger than these values, there is normally no functional recovery. Clinically, there has been little progress in developing new techniques that increase the level of functional recovery following peripheral nerve injury. This paper examines the efficacies and limitations of sensory nerve grafts and various other techniques used to induce functional neurological recovery, and how these might be improved to induce more extensive functional recovery. It also discusses preliminary data from the clinical application of a novel technique that restores neurological function across long nerve gaps, when repairs are performed at long times post-trauma, and in older patients, even under all three of these conditions. Thus, it appears that function can be restored under conditions where sensory nerve grafts are not effective.

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“…The emergence of tissue engineering techniques that consist of three essential elements, seed cells, growth factors, and scaffolds, provides new ideas and directions for the clinical treatment of diseases [ 5 ]. Many kinds of mesenchymal stem cells (MSCs), such as bone marrow mesenchymal stem cells (BMSCs) [ 6 ], adipose-derived MSCs [ 7 ], and peripheral blood-derived MSCs [ 8 ], have been successfully extracted and applied in various basic studies. Among these cell types, BMSCs are the most widely used MSCs.…”

Section: Introductionmentioning

confidence: 99%

Fibroblast growth factor 2–induced human amniotic mesenchymal stem cells combined with autologous platelet rich plasma augmented tendon-to-bone healing

Zhang

Liu

Tang

et al. 2020

Journal of Orthopaedic Translation

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Objective The purpose of this study was to explore the effect of fibroblast growth factor 2 (FGF-2) on collagenous fibre formation and the osteogenic differentiation of human amniotic mesenchymal stem cells (hAMSCs) in vitro, as well as the effect of FGF-2–induced hAMSCs combined with autologous platelet-rich plasma (PRP) on tendon-to-bone healing in vivo. Methods In vitro, hAMSCs were induced by various concentrations of FGF-2 (0, 10, 20, and 40 ng/ml) for 14 days, and the outcomes of ligamentous differentiation and osteogenic differentiation were detected by quantitative real-time reverse transcription PCR, Western blot, immunofluorescence, and picrosirius red staining. In addition, a lentivirus carrying the FGF-2 gene was used to transfect hAMSCs, and transfection efficiency was detected by quantitative real time reverse transcription PCR (qRT-PCR) and Western blot. In vivo, the effect of hAMSCs transfected with the FGF-2 gene combined with autologous PRP on tendon-to-bone healing was detected via histological examination, as well as biomechanical analysis and radiographic analysis. Results In vitro, different concentrations of FGF-2 (10, 20, and 40 ng/ml) all promoted the ligamentous differentiation and osteogenic differentiation of hAMSCs, and the low concentration of FGF-2 (10 ng/ml) had a good effect on differentiation. In addition, the lentivirus carrying the FGF-2 gene was successfully transfected into hAMSCs with an optimal multiplicity of infection (MOI) (50), and autologous PRP was prepared successfully. In vivo, the hAMSCs transfected with the FGF-2 gene combined with autologous PRP had a better effect on tendon-to-bone healing than the other groups ( p < 0.05), as evidenced by histological examination, biomechanical analysis, and radiographic analysis. Conclusion hAMSCs transfected with the FGF-2 gene combined with autologous PRP could augment tendon-to-bone healing in a rabbit extra-articular model. The translational potential of this article hAMSCs transfected with the FGF-2 gene combined with autologous PRP may be a good clinical treatment for tendon-to-bone healing, especially for acute sports-related tendon–ligament injuries.

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Seeding decellularized nerve allografts with adipose-derived mesenchymal stromal cells: An in vitro analysis of the gene expression and growth factors produced

Cited by 25 publications

References 57 publications

Adipose tissue stem cells in peripheral nerve regeneration—In vitro and in vivo

Adipose tissue stem cells in peripheral nerve regeneration—In vitro and in vivo

Restoration of Neurological Function Following Peripheral Nerve Trauma

Fibroblast growth factor 2–induced human amniotic mesenchymal stem cells combined with autologous platelet rich plasma augmented tendon-to-bone healing

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