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This study describes and validates a new method of effectively seeding decellularized nerve allografts with mesenchymal stromal cells. This method is reproducible, distributes cells homogenously over the graft, and does not traumatize the intraneural architecture of the allograft. Use of this validated seeding technique will permit critical comparison of graft outcomes.

Level IV, retrospective case series.

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Ultrasound measurement of muscle atrophy was highly correlated with the criterion standard muscle mass ratio and was also significantly correlated with isometric tetanic force. Histologic evaluation confirmed the regeneration pattern observed with ultrasound. The authors propose that ultrasound can be used as a valid alternative to muscle mass ratio to study muscle atrophy after nerve injury in a less-invasive and more animal-friendly manner.

US may be a valuable tool for evaluating clinically suspected sural nerve damage after ankle stabilization surgery. Sural nerve abnormalities are seen in patients with postsurgical neuropathic pain. Muscle Nerve 57: 407-413, 2018.