Adipose tissue stem cells in peripheral nerve regeneration—In vitro and in vivo

Rhode, Sophie Charlotte; Beier, Justus P.; Ruhl, Tim

doi:10.1002/jnr.24738

Cited by 40 publications

(33 citation statements)

References 112 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…After nerve injury, SCs transdifferentiate into so-called repair SCs, and promote lysis of myelin, attract macrophages to participate in phagocytosis and secrete neurotrophic factors, such as NGF, to support and control neuronal function and guide axonal regeneration and axonal re-myelination (6,28,36,37). Repair SCs are considerably different from immature SCs.…”

Section: Discussionmentioning

confidence: 99%

“…However, owing to limitations in SC separation, purification and proliferation technology, there is an urgent requirement for abundant alternative cells with lower immunogenicity. Regenerative medicine has recently been applied to solve these issues (6). Adipose-derived stem cells (ADSCs) have been focused on because of their multipotency and ease of harvest.…”

Section: Introductionmentioning

confidence: 99%

“…A recent study on a rat PNI model has demonstrated that FA promoted proliferation and migration of SCs and secretion of NGF to improve the repair of peripheral nerve damage (18). SCs transform into a repair phenotype at the site of the injured nerve to promote nerve regeneration (6). Then, NGFs promote autophagy of SCs through the p75 neurotrophin receptor (p75NTR) / AMPK / mechanistic target of rapamycin (mTOR) signaling pathway to accelerate removal and phagocytosis of myelin debris and promote the regeneration of axons and myelin in the early stages of PNI (19).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Effective in vitro differentiation of adipose-derived stem cells into Schwann-like cells with folic acid supplementation

et al. 2021

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Effective in vitro differentiation of adipose-derived stem cells into Schwann-like cells with folic acid supplementation

et al. 2021

View full text Add to dashboard Cite

“…Adipose-derived Mesenchymal stem cells' (Ad-MSCs) source is the stroma of fat tissue, making them accessible and abundant [43,48]. In comparison with BM-MSCs, Ad-MSCs can be collected in a liposuction, a less invasive manner and in higher quantities [16,48,49]. Besides, the capability of differentiation and proliferation are much higher than other sources of MSCs [16,49].…”

Section: Adipose-derived Mesenchymal Stem Cellsmentioning

confidence: 99%

“…Another important advantage is that location and age of the donor does not influence the therapeutic effect. For these reasons, Ad-MSCs are attractive for regenerative medicine, tissue engineering and peripheral nerve regeneration [48,49].…”

Section: Adipose-derived Mesenchymal Stem Cellsmentioning

confidence: 99%

Peripheral Nerve Injury Treatments and Advances: One Health Perspective

Lopes¹,

Sousa²,

Alvites³

et al. 2021

Preprint

View full text Add to dashboard Cite

Peripheral nerve injuries (PNI) can have several etiologies, such as trauma and iatrogenic interventions that can lead to the loss of structure and/or function impairment. These changes can cause a partial or complete loss of motor and sensory functions, physical disability, and neuropathic pain, what in turn can affect the quality of life. For those reasons, PNI is a major public health concern. This review aims to revisit the concepts associated with the PNI. First, the anatomy of the peripheral nerve is detailed to explain the different types of injury. Then, some of the available therapeutic strategies are explained, including surgical methods, pharmacological therapies, and the use of cell-based therapies alone or in combination with biomaterials in the form of tube guides. Nevertheless, even with the various available treatments, it is difficult to achieve a perfect outcome with complete functional recovery. This review aims to explain the urge for new approaches and to understand the methods to evaluate nerve regeneration in a One Health perspective. In vitro models followed by in vivo models are very important to be able to translate the achievements to human medicine.

show abstract

Injectable Prevascularized Mature Adipose Tissues (iPAT) to Achieve Long‐Term Survival in Soft Tissue Regeneration

Louis

Sowa

Irie

et al. 2022

Adv Healthcare Materials

View full text Add to dashboard Cite

Soft tissue regeneration remains a challenge in reconstructive surgery. So far, both autologous fat implantations and artificial implants methods used in clinical applications lead to various disadvantages and limited lifespan. To overcome these limitations and improve the graft volume maintenance, reproducing a mature adipose tissue already including vasculature structure before implantation can be the solution. Therefore, injectable prevascularized adipose tissues (iPAT) are made from physiological collagen microfibers mixed with human mature adipocytes, adipose‐derived stem cells, and human umbilical vein endothelial cells, embedded in fibrin gel. Following murine subcutaneous implantation, the iPAT show a higher cell survival (84% ± 6% viability) and volume maintenance after 3 months (up to twice heavier) when compared to non‐prevascularized balls and liposuctioned fat implanted controls. This higher survival can be explained by the greater amount of blood vessels found (up to 1.6‐fold increase), with balanced host anastomosis (51% ± 1% of human/mouse lumens), also involving infiltration by the lymphatic and neural vasculature networks. Furthermore, with the cryopreservation possibility enabling their later reinjection, the iPAT technology has the merit to allow noninvasive soft tissue regeneration for long‐term outcomes.

show abstract

Adipose tissue stem cells in peripheral nerve regeneration—In vitro and in vivo

Abstract: This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

Cited by 40 publications

References 112 publications

Effective <i>in vitro</i> differentiation of adipose-derived stem cells into Schwann-like cells with folic acid supplementation

Effective <i>in vitro</i> differentiation of adipose-derived stem cells into Schwann-like cells with folic acid supplementation

Peripheral Nerve Injury Treatments and Advances: One Health Perspective

Injectable Prevascularized Mature Adipose Tissues (iPAT) to Achieve Long‐Term Survival in Soft Tissue Regeneration

Contact Info

Product

Resources

About